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Journal Cover Journal of Applied Ichthyology
  [SJR: 0.482]   [H-I: 36]   [6 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0175-8659 - ISSN (Online) 1439-0426
   Published by John Wiley and Sons Homepage  [1597 journals]
  • Effect of cellulase, phytase and pectinase supplementation on growth
           performance and nutrient digestibility of rainbow trout (Oncorhynchus
           mykiss, Walbaum 1792) fry fed diets containing canola meal
    • Authors: N. O. Yigit; E. Keser
      Abstract: This experiment was conducted to evaluate the effects of supplementing exogenous enzymes on growth, feed conversion ratio (FCR) and apparent nutrient digestibility in rainbow trout (Oncorhynchus mykiss) fry diets containing 32% canola meal. Five experimental diets (including a control diet containing no enzymes) were prepared as isonitrogenous (44% crude protein) and isocaloric (4000 kcal DE kg1). The four other diets contained either cellulase, phytase, pectinase or an enzyme mix (a mixture of cellulase, phytase and pectinase in the same ratio). The feeding trial was conducted in triplicate for 12 weeks in 15 tanks (100‐L). At the beginning of the experiment 20 rainbow trout fry (initial weight 1.23 g) were stocked into each tank. Mean water temperature in the rearing tanks was 11°C and water flow in each tank was 6 L min−1. At the end of the experiment the growth parameters and FCR displayed no significant differences in enzyme supplementation (P > 0.05). In addition, no differences were observed in dry matter, protein, or lipid digestibility with enzyme supplementation (P > 0.05). The results of this study showed that the addition of pectinase, phytase, cellulase or an enzyme mix to a diet containing 32% canola meal had no effect on growth, feed efficiency or dry matter, protein, or lipid digestibility in rainbow trout fry.
      PubDate: 2016-04-15T07:14:24.909273-05:
      DOI: 10.1111/jai.13088
  • Growth, parasitic infection and hematology in Colossoma macropomum Cuvier,
           1818 fed diets containing Allium sativum
    • Abstract: The aim of this study was to investigate the effects of a dietary Allium sativum (garlic) addition on growth, parasite infestation and blood parameters in Colossoma macropomum (tambaqui) using 12 net‐cages (1 m3). There were four treatments with three replicates each (15 fish per replicate). Tambaqui of 112.4 g mean weight were fed for 45 days with diets containing 0, 15, 30 and 45 g of fresh garlic kg−1 feed. Garlic did not affect the tambaqui survival, growth, feed conversion, body condition, plasma glucose levels, total plasma protein levels or red blood cells. Total leukocyte and lymphocyte numbers decreased in fish fed diets containing 45 g garlic kg−1 feed, but neutrophils decreased in fish fed diets containing 15, 30 and 45 g garlic kg−1 diet, while the number of immature leukocytes increased in fish fed 30 g and 45 g garlic kg−1diet. Gills in fish fed 30 and 45 g kg−1 diet had lower infestation (52% and 63%, respectively) of Anacanthorus spathulatus (Monogenea) compared to controls. The application of garlic in diets improved the response of leukocytes and enhanced the resistance to infections by monogeneans.
      PubDate: 2016-04-15T07:14:02.225047-05:
      DOI: 10.1111/jai.13086
  • A new record of dasyatid fish from the Sea of Marmara: Tortonese's
           stingray, Dasyatis tortonesei Capapé, 1975 (Dasyatidae)
    • PubDate: 2016-04-15T07:13:39.377548-05:
      DOI: 10.1111/jai.13087
  • Ovarian development of albacore (Thunnus alalunga Bonnaterre, 1788) in the
           Eastern Mediterranean Sea: a histological characterisation
    • Authors: F. S. Karakulak; T. Akayli, I. K. Oray, R. E. Yardimci
      Abstract: In order to investigate the stages of oogenesis in the albacore Thunnus alalunga, 129 females (64.0‒83.5 cm fork length‐FL) were captured by gill nets from April to August 2002–2008, in the eastern Mediterranean Sea. Based on histological examination of the ovaries, a total of seven ovarian developmental stages were identified: perinucleolar, lipid, early vitellogenesis, late vitellogenesis, migratory nucleus, pre‐hydrated and hydrated. The monthly gonadosomatic index values and the results of the histological analysis indicated that the females mature in May. The spawning stage becomes predominant in June and reaches a maximum in July. The evidence reported here indicates that the size of females at first sexual maturity was lower for the Mediterranean albacore stock than for the Pacific and Atlantic stocks.
      PubDate: 2016-04-15T07:13:30.967876-05:
      DOI: 10.1111/jai.13076
  • Trapped in their own ‘home’: unexpected records of intertidal
           fish desiccation during low tides
    • PubDate: 2016-04-15T07:12:38.959231-05:
      DOI: 10.1111/jai.13074
  • Length–weight relationships and condition factors of two fish
           species from the southern Caspian Sea basin: Alburnoides samiii
           Mousavi‐Sabet, Vatandoust & Doadrio, 2015 and Ponticola iranicus
           Vasil′eva, Mousavi‐Sabet & Vasil′ev, 2015
    • Abstract: The present study describes the length–weight relationships (LWRs) and condition factors (K) of Alburnoides samiii and Ponticola iranicus in the Sefidroud River from the southern Caspian Sea basin, September 2013 to August 2014. This study presents the first known reference in the FishBase database regarding the LWRs and condition factors for these two indigenous species.
      PubDate: 2016-04-15T07:12:12.543259-05:
      DOI: 10.1111/jai.13083
  • Length‐weight relationships of three cyprinid fish species from
           southwestern China
    • Authors: H. Y. Cao; Z. Yang, L. Cai, L. Pan
      Abstract: Length–weight relationships (LWRs) were determined for three cyprinid fish species [Acrossocheilus yunnanensis (Regan, 1904), Onychostoma simum (Sauvage & Dabry de Thiersant, 1874) and Spinibarbus sinensis (Bleeker, 1871)] from southwestern China. Samples were obtained with various fishing gear (set nets, drift gill nets, fish cages, hook and electrofishing). Prior to this study, the LWRs for these three species (A. yunnanensis, O. simum and S. sinensis) were unknown.
      PubDate: 2016-04-15T07:12:00.441087-05:
      DOI: 10.1111/jai.13085
  • Morphological divergence in Indian oil sardine, Sardinella longiceps
           Valenciennes, 1847– Does it imply adaptive variation?
    • Authors: S. Sukumaran; A. Gopalakrishnan, W. Sebastian, P. Vijayagopal, S. Nandakumar Rao, N. Raju, S. Ismail, E. M. Abdussamad, P.K. Asokan, K. P. Said Koya, P. Rohit
      Abstract: The Indian oil sardine, Sardinella longiceps, is an important pelagic species in Indian waters, and shows divergent morphology while in sympatry. The reasons behind this divergent morphology were investigated using morphometric, genetic and nutritional analyses. Twenty‐one morphometric characters (as percentage of standard length) and eight meristic characters were studied in the three variants to assess whether they are significantly diverged. Distinct clustering of morphotypes was evident in the principal component analysis on log‐transformed ratios of morphological characters with PC1 and PC2, explaining 50.7% and 17.6% of the total morphological variation, respectively. PC1 was highly correlated with the distance from snout to anal origin, depth at dorsal, distance from snout to pelvic and distance from snout to first dorsal. PC2 was highly correlated with head length, caudal width and anal depth. Analysis of similarities (ANOSIM) was conducted using log‐transformed morphometric ratios, with the results showing the clusters to be well differentiated (R = 0.511; P 
      PubDate: 2016-04-15T07:11:57.252282-05:
      DOI: 10.1111/jai.13060
  • Isolation and characterization of ten polymorphic microsatellite loci from
           Gymnocypris chui and cross‐amplification in seven highly specialized
           Schizothoracinae fishes
    • Authors: R. Y. Zhang; K. Zhao
      PubDate: 2016-04-15T07:11:03.238099-05:
      DOI: 10.1111/jai.13066
  • Length–weight relationship of 10 freshwater fish species in
           Amazonian streams, Trombetas River basin (Brazil)
    • Authors: D. C. O. Rosa; B. E. Soares, M. P. Albrecht, E. P. Caramaschi
      Abstract: Length–weight relationships (LWR) were analyzed for 10 fish species from 26 Amazonian streams located in four micro‐basins in the Trombetas River basin, Pará State, Brazil. LWRs were calculated based on 1831 individuals sampled between 2010 and 2012 using a manual trawl. The coefficients of proportionality and allometry were within the ranges estimated for most fishes. No previous LWR information was available for these species in the literature.
      PubDate: 2016-04-07T23:11:50.168845-05:
      DOI: 10.1111/jai.13082
  • The ontogeny of neurosensory structures in larval Atlantic halibut,
           Hippoglossus hippoglossus (Linnaeus, 1758)
    • Authors: A. J. Borsky; I. R. Bricknell
      Abstract: The ontogenic development of neurosensory structures in larval Atlantic halibut, Hippoglossus hippoglossus (L.) was examined using microscopy imaging techniques and analysis. Larval samples were preserved at developmental stages of 444, 486, 528, 570, 612, and 666 degree‐days, respectively. Larvae were measured and photographed macroscopically. The fish were then embedded in paraffin for histological processing. 5 μm sequential sections of the anterior of the larvae were cut and stained using hematoxylin and eosin dyes. The resulting sections were examined using an Olympus BX60 compound microscope. The development of neurosensory structures such as neuromasts and sensory papillae were observed throughout all developmental stages of larvae and early development of the lateral line was observed at 666 degree‐days. Neuromast frequency increased as larvae matured, suggesting that larvae were approaching their first feeding event towards the end of the sampling period. Possible implications of development on larval first feeding are discussed.
      PubDate: 2016-04-07T01:41:40.94088-05:0
      DOI: 10.1111/jai.13064
  • Occurrence of chromatophoroma or chromatophoromatosis in Sardinella
           longiceps (Valenciennes, 1847) from Tamilnadu, southeast coast of India
    • Authors: V. Singaravel; A. Gopalakrishnan, R. Vijayakumar, K. Raja, S. Asrafuzzaman
      Abstract: The study was undertaken to analyse the prevalence of skin tumours in Sardinella longiceps, and to investigate the tumour type occurrence, distribution and histology. Fish samples were collected from three different landing centres (Cuddalore, Parangipettai and Nagapattinam) of Tamilnadu, southeast coast of India, from January 2014 to December 2014. A total of 192 661 individuals were examined, 750 of which had tumours for an overall prevalence of 0.39%. By station, higher prevalence was observed in Parangipettai (0.41%) followed by Cuddalore (0.39%) and Nagapattinam (0.36%). Chromatophoromas were predominant among the types of tumours in sardines. The multi‐coloured cutaneous tumour masses were in the head and body skin. Among the chromatphoromas, the erythrophoromas were most abundant followed by melanophoromas, iridophoromas and leucophoromas. Histologically, the tumour lesions were characterized by hyperplastic pigment cells intermingled with fibroblasts. The gross‐morphological and histopathological diagnoses of these lesions were compatible with erythrophoroma, melanophoroma, iridophoroma and leucophoroma.
      PubDate: 2016-04-07T01:41:18.148641-05:
      DOI: 10.1111/jai.13084
  • Diet composition of greater weever, Trachinus draco (Linnaeus, 1758)
           captured in the eastern‐central Adriatic Sea in relation to fish
           size, season and sampling area
    • Abstract: The diet compositions of greater weever, Trachinus draco, from the eastern‐central Adriatic Sea, were investigated with respect to seasons, fish size and sampling locations. Analyzed were the stomach contents of 592 specimens, total length (TL) of 9.9–31.2 cm, collected by commercial bottom trawls from January to December 2008. The frequency of empty stomachs varied significantly with the season (43.3% maximum during winter; 6.8% minimum during summer). Prey items identified in the stomachs belonged to seven major taxonomic groups: Bivalvia, Cephalopoda, Decapoda, Mysidacea, Isopoda, Amphipoda and Teleostei. Decapods were the most important prey (%IRI = 72.8) followed by teleosts (%IRI = 16.4) and mysids (%IRI = 15.0); other prey groups were only occasionally ingested. Diet composition showed little seasonal variation; decapods were the most important prey in all seasons. Fish size was the most important factor influencing the composition of the diet. Small individuals (
      PubDate: 2016-04-07T01:40:52.425632-05:
      DOI: 10.1111/jai.13081
  • The effects of endosulfan on P450 1A gene expression, antioxidant enzymes
           activity and histopathological alterations in liver of Persian sturgeon
           (Acipenser persicus Borodin, 1987)
    • Authors: R. Safari; M. Khalili, M. Reza Imanpour, M. Pourkazemi
      Abstract: The effects of 14 days exposure to sublethal concentrations of endosulfan (10 and 40 μg L−1) were investigated in mRNA‐ P450 1A expression, antioxidant enzymes (SOD and CAT) activity and histopathological alterations of Persian sturgeon (Acipenser persicus) fingerlings with weights of 3–5 g. The results illustrated that the relative mRNA‐ P450 1A expression level significantly increased (P 
      PubDate: 2016-04-06T03:25:20.12785-05:0
      DOI: 10.1111/jai.13072
  • Length–weight relationships of two fish species from the middle
           reaches of the Jinsha River, China
    • Authors: T. B. Zhu; D. G. Yang
      Abstract: This study provides the first report of length–weight relationships (LWRs) for Pseudogyrinocheilus prochilus (Sauvage & Dabry de Thiersant, 1874) and Jinshaia sinensis (Sauvage & Dabry de Thiersant, 1874) collected from the middle reaches of the Jinsha River, southwest China. Samples were obtained between June 2005 and November 2006. A total of 491 specimens belonging to two fish species were analyzed. LWRs were W = 0.0170L2.970 (r2 = 0.980) for P. prochilus and W = 0.0194L2.849 (r2 = 0.977) for J. sinensis. Prior to this study, the LWRs for P. prochilus were unknown. In addition, new maximum size for J. sinensis was provided.
      PubDate: 2016-04-06T03:19:05.334064-05:
      DOI: 10.1111/jai.13079
  • Patterns of reproduction and growth of the catfish Iheringichthys labrosus
           (Lütken, 1874) after a reservoir formation
    • Authors: H. Soares de Santana; A. Cantarute Rodrigues, C. Dei Tos
      Abstract: This work contributes to the knowledge of the reproduction, growth parameters and mortality rates of Iheringichthys labrosus in a newly‐formed reservoir. A total of 554 males and 1227 females were collected over 12 consecutive months, 1998–1999, from sites in the Corumbá Reservoir, Brazil, using gillnets (meshes: 2.4–16 cm). Information on each individual, i.e. standard length (cm), weight (g), sex, and gonadal development phase was recorded. The pectoral spines were removed to estimate age. The number of juveniles and adults, males and females, reproductive sites and seasons were estimated. First maturation length was estimated using a likelihood function fitted by binomial distribution. Growth parameters were estimated using the von Bertalanffy equation. Total instantaneous mortality was obtained through a linearized catch curve method. Standard length varied from 6.0 to 20.5 cm. Growth showed negative allometry for both sexes. The reproduction period was August to December in all environments sampled and first maturation length was 11.5 cm. All individuals were adults with 17.0 cm standard length. Ages varied from zero to 7 years. Asymptotic length, growth coefficient and t0 for the entire population were 27.79 cm, 0.12 and −2.64, respectively. Instantaneous and annual mortality rates were 0.90 and 0.59, respectively.
      PubDate: 2016-04-02T02:42:10.11435-05:0
      DOI: 10.1111/jai.13059
  • Cold storage effects on flesh quality of rainbow trout Oncorhynchus mykiss
           (Walbaum, 1792) fed diets containing different vegetable oils
    • Authors: M. Yildiz; I. Kose, G. Issa, T. Kahraman, E. Guven, M. A. Baltaci, K. Yuruten
      Abstract: The intention of this experiment was to assess the effects of different sources of dietary lipid on the fatty acid composition of the fillet and liver and the flesh quality traits of rainbow trout (Oncorhynchus mykiss) after a 70‐day feeding period. Four iso‐nitrogenous (approx. 51% crude protein) and iso‐lipidic (approx. 14% crude lipid) experimental diets were formulated. The control diet contained only fish oil (FO) as the primary lipid source. In the other three dietary treatments, fish oil was replaced by 100% (LO30/SO35/SFO35) and 70% (FO30/LO35/SO35 or FO30/SO35/SFO35) sesame oil (SO), linseed oil (LO), or sunflower oil (SFO). Triplicate groups of 40 rainbow trout (~46 g) held under similar culture conditions were hand‐fed daily to apparent satiation for 70 days. At the end of the feeding trials, no difference in growth performance among experimental groups was noted (P > 0.05). There were some differences in the proximate composition of fish fillets (P 
      PubDate: 2016-04-02T02:41:43.972855-05:
      DOI: 10.1111/jai.13037
  • Elasmobranch captures in shrimps trammel net fishery off the Gulf of
           Gabès (Southern Tunisia, Mediterranean Sea)
    • Authors: B. Saidi; S. Enajjar, M. N. Bradai
      Abstract: Small‐scale fisheries are generally promoted as a sustainable alternative to large‐scale industrial fisheries. However, there is recent growing evidence that small‐scale fisheries may be the largest threat to marine species of conservation concern. The objective of this study was to evaluate the potential impact of the trammel net fishery on elasmobranchs in the Gulf of Gabès, Southern Tunisia. Data are based on 191 shrimp trammel net set (40 mm stretched mesh size) surveys conducted aboard commercial fishing vessels from May to July 2009. Five species of the small coastal elasmobranchs (Mustelus mustelus (Linnaeus, 1758), Mustelus punctulatus Risso 1827, Dasyatis pastinaca (Linnaeus, 1758), Dasyatis marmorata (Steindachner, 1892) and Torpedo torpedo (Linnaeus, 1758)) and two species from the large coastal shark (Carcharhinus plumbeus (Nardo, 1827) and Carcharhinus brevipinna (Müller & Henle, 1839)) were recognized as by‐catch in this fishery. Elasmobranch by‐catch was dominated by sharks (90.3%), smoothhound sharks Mustelus sp. being by far the most important (88.9%) and reflecting their abundance in the area; 58% of the sets caught at least one specimen, with 4.8 ± 1.3 caught per set. Captures were composed essentially of neonate and juvenile sharks, while the batoids were dominated by mature individuals. This study shows that shrimp trammel nets represent a considerable source of mortality for early life stages of elasmobranch species in the Gulf of Gabès. Additionally, there was a high density of neonates and small juvenile M. mustelus in the Sfax zone, suggesting that these nearshore waters are a nursery grounds for smoothhound sharks. Further research should focus on the incidents of by‐catch and evaluate the potential solutions to allow trammel net fisheries to coexist alongside the elasmobranch species.
      PubDate: 2016-04-02T02:41:21.642009-05:
      DOI: 10.1111/jai.13061
  • Length–weight relationship of five commercially important freshwater
           fish species in the Kashmir Valley, India
    • Authors: A. Bashir; N. K. Sharma, B. S. Bisht, R. Singh, J. I. Mir, M. S. Akhtar
      Abstract: Length–weight relationships were analysed for five commercially important freshwater fishes, namely, Bangana diplostoma (Heckel, 1838), Schizopyge niger (Heckel, 1838), Schizothorax curvifrons (Heckel, 1838), Schizothorax plagiostomus (Heckel, 1838) and Glyptosternon reticulatum (McClelland, 1842) from different water bodies in the Kashmir Valley, India. A total of 610 samples were collected between October 2013 and May 2015 using various indigenous cast nets. Of these five species, Schizopyge niger has a new maximum length record for the FishBase LWR database.
      PubDate: 2016-04-02T02:40:58.438831-05:
      DOI: 10.1111/jai.13065
  • Length–weight relationships of two fish species from the Yangtze
           River, China
    • Authors: L. Y. Wu; J. R. Lan, C. Cheng, Q. S. Tan
      Abstract: Fish samples for the study were collected from the Yangtze River in China. Length–weight relationships were determined for two endemic fish species (Leiocassis longirostris Günther, 1864 and Schistura fasciolata Nichols & Pope, 1927) for the first time.
      PubDate: 2016-04-02T02:40:38.664162-05:
      DOI: 10.1111/jai.13068
  • The effects of different cycles of starvation and re‐feeding on
           growth and body composition in rainbow trout (Oncorhynchus mykiss,
           Walbaum, 1792)
    • Abstract: This study was designed to investigate the effects of starvation and re‐feeding cycles on the growth performance and body chemical composition of Oncorhynchus mykiss juveniles. A total of 360 juveniles with initial mean weights (IW) of 8.46 ± 0.07 g (n = 360) were stocked into 400‐L tanks in triplicate for each group, with 30 juveniles per tanks. The control group received regular feed, as is the common practice. The three other groups were periodically starved: 1 day starvation followed by 6 days re‐feeding (S1), 2 days starvation followed by 5 days re‐feeding (S2) and 3 days starvation followed by 4 days re‐feeding (S3). The experiment lasted for 10 weeks, over the course of which the water flow rate was 4 L min−1 and the water quality parameters determined as: temperature 14.4 ± 1.1°C, oxygen 8.2 ± 0.4 mg L−1 and pH 7.5 ± 0.2. At the end of the study, S1 had the best growth performance (final weight, specific growth rate, average daily growth) of all test groups (P 
      PubDate: 2016-04-01T05:02:54.597565-05:
      DOI: 10.1111/jai.13045
  • Effects of β‐xylanase and 6‐phytase on digestibility,
           trace mineral utilisation and growth in juvenile red tilapia, Oreochromis
           niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852), fed declining
           fishmeal diets
    • Authors: J. L. Wallace; F. J. Murray, D. C. Little
      Abstract: In response to the global sustainability drive to lower fishmeal (FM) inclusion in aquatic feeds, exogenous enzymes can improve nutrient digestibility in monogastric plant‐based diets. A 80‐day experiment was conducted to evaluate the combined effects of xylanase and phytase on digestibility, trace mineral utilisation and growth in juvenile red tilapia, Oreochromis niloticus x O. mossambicus, (48.8 g ± 13.9; μ ± STD) fed declining FM diets. Basal diets were formulated to contain 0, 3 and 5% FM with and without xylanase (0.385 g kg−1) and phytase (0.075 g kg−1), forming six treatments. Each treatment was randomly assigned to four replicates, 20 fish tank−1; mean water temperature 28.98 ± 0.73°C. Although the size of the effects was modest, growth performances (feed intake, FCR, growth rate) decreased with lower FM levels (P 
      PubDate: 2016-04-01T05:02:08.776559-05:
      DOI: 10.1111/jai.13055
  • Occurrence and prevalence of Philometra filiformis (Stossich, 1896) on
           Pagellus erythrinus (Linnaeus, 1758) in the Southern Tyrrhenian Sea
    • Authors: F. Marino; B. Busalacchi, T. Bottari, P. Rinelli, G. Gaglio
      Abstract: In this paper the host‐parasite relationship between Philometra filiformis and the common pandora Pagellus erythrinus from the Southern Tyrrhenian Sea were studied. A total of 305 (257 females and 48 males) specimens of common pandora were sampled from June 2009 to April 2011 from commercial landings as well as from bottom trawl surveys. A total of 39 P. erythrinus were parasitized (mean prevalence, P = 12.80%; mean abundance, A = 0.19), with the parasite occurring throughout the study area and with wide fluctuations in prevalence values ranging from 0 to 28.60%. Prevalence increased with age of the host, with most parasites (27.78%) found in the older common pandora whereby P. filiformis parasitized only the females. A significant correlation between prevalence, abundance and maturity stages of the fish was evident, with most parasites found in mature/spawning specimens. The varying degrees of gonadic damage caused by this nematode reveal a serious threat to the reproductive success of P. erythrinus.
      PubDate: 2016-03-28T02:21:25.900212-05:
      DOI: 10.1111/jai.13069
  • Age, growth, mortality and population structure of silver croaker Pennahia
           argentata (Houttuyn, 1782) and red bigeye Priacanthus macracanthus Cuvier,
           1829 in the north‐central Taiwan Strait
    • Abstract: The main objectives of this study were to estimate the age, growth, mortality and population structure of silver croaker Pennahia argentata (Houttuyn, 1782) and red bigeye Priacanthus macracanthus Cuvier, 1829 and to explore the factors causing the fluctuation of the fish populations and the ecological parameters. The two species were randomly sampled, with 80~120 individuals of each species captured every month from a single trawler fishery (mesh size 250 mm), March to November 2006, in the north‐central Taiwan Strait. A total of 994 speciments of silver croaker and 851 speciments of red bigeye were collected. Compared with previous studies, the mean length and weight, mean age, the minimum size at first sexual maturity, the asymptotic length (L∞) and weight (W∞) of the two species has decreased in recent decades, indicating that the populations were younger, smaller and earlier in sexual maturity. Meanwhile, the growth coefficient (K) increased, the mortality coefficients (Z, F, M) were higher, and the exploitation rate (E) indicated overfishing. Fishing is a key driving force that can cause abundant fluctuations and changes in ecological characteristics in these two demersal fishes in this area. Accordingly, the traditional fishery management approach, such as the reduction in fishing effort, coupled with the Ecosystem‐based Fishery Management (EBFM) should be implemented to achieve sustainable demersal fisheries.
      PubDate: 2016-03-28T02:21:06.721581-05:
      DOI: 10.1111/jai.13053
  • Initial study on catch, species composition and reproductive biology of
           fishes off the south‐west coast of Sri Lanka, targeted by ring nets
           while utilizing natural floating objects
    • Authors: K. B. E. Chathutika; D. C. T. Dissanayake
      Abstract: This study evaluates the catch rates, species composition and reproductive biology of flotsam‐associated fishes targeted by ring nets off the southwest coast of Sri Lanka. Catch and number of multiday boats operating with ring nets were collected at Beruwala fishery harbor on the southwest coast of Sri Lanka, March to October 2013, by making fortnightly field visits. Fish samples were collected randomly from the unloaded ring net catches to analyze reproductive biology. Twelve fish species belonging to four major families: Scombridae, Carangidae, Coryphaenidae and Balistidae were identified in the ring net catches and five species, Decapterus russelli, Katsuwonus pelamis, Thunnus albacares, Elagatis bipinnulata and Canthidermis maculatus were predominant. An average of 25 ± 12% multiday boats landing at the Beruwala fishery harbor operated with ring nets each month, with an average monthly catch rate fluctuating from 730 ± 101 to 3924 ± 1094 kg per boat per trip. Catch rates of tunas and carangids were significantly higher than the other fish groups (anova; d.f. = 3, P 
      PubDate: 2016-03-28T02:20:43.500923-05:
      DOI: 10.1111/jai.13054
  • Catch composition and some other aspects of bottom trawl fishery in
           Sığacık Bay, central Aegean Sea, eastern Mediterranean
    • Abstract: The objective of the study was to determine the features of the bottom trawl fishery such as catch composition, catch per unit effort (CPUE) and the discard ratios in the Sığacık Bay fishery, central Aegean Sea. A commercial trawler conducted trawl operations from October 2008 to April 2009, with a total of 18 trawls in autumn, winter and spring (six hauls per season). In total, 84 species were identified, 47 of which belonged to the bony fishes, while other groups contributed nine (cartilaginous fish), 10 (cephalopods), 13 (crustaceans), four (echinoderms) and one (porifera) species. Some 49 species were discarded regardless of length. Total catch was 3693.2 kg of which 66.8% was marketed. Among the commercial species, rose shrimp (Parapenaeus longirostris) dominated the catch composition with 998 kg. However, discards accounted for 33.2% of the total catch biomass, with the majority represented by Lampanyctus crocodilus with 97 kg total weight. The study area is considered one of the most efficient trawling zones in the Aegean Sea and thus requires long‐term monitoring in terms of fish stocks and trawl discards for the preparation of well‐informed decisions for a sustainable fishery.
      PubDate: 2016-03-24T02:42:24.381832-05:
      DOI: 10.1111/jai.13042
  • Microbiological changes in whiting (Merlangius merlangus Linneaus, 1758)
           fillets during short‐term cold storage and a traditional
           ‘pastrami‐like’ treatment
    • Abstract: The objective of this study was to test the effects of salt‐dried whiting (Merlangius merlangus) fillet storage when treated with a special paste and stored covered. For this purpose whiting fillets were salt‐dried at 4–6°C for 15 days. A subsequent test series involved a paste mixture prepared from ground fenugreek, cumin seeds, black pepper, red pepper powder and garlic. The fillets were coated with this paste and air‐dried (15–20°C) for 5 days. All microbiological changes during this drying period were noted. The aerobic mesophilic bacterial counts decreased significantly (P 
      PubDate: 2016-03-24T02:41:58.441136-05:
      DOI: 10.1111/jai.13043
  • Hatchery performance in a major rainbow trout (Oncorhynchus mykiss,
           Walbaum, 1792) seed production area of Turkey
    • Abstract: This study aims to determine the production characteristics and performance of rainbow trout hatcheries in Seydikemer, Muğla, Turkey where over half of the total production of eyed eggs and juveniles take place. For this purpose six hatcheries with different production scales were selected and coded A, B, C, D, E and F (with capacities of 60, 15, 5.6, 4, 0.95 and 0.6 million juveniles per year, respectively). From each hatchery, 20 females were selected and their egg qualities (egg number, diameter and weight, fertilization, eyed egg, hatching, swim‐up fry and survival rates at 120 days after hatching) and juvenile growth rates were monitored until the 120th day after hatching under each farms own conditions. The sperm characteristics of 10 broodstock males from each hatchery were also examined. Although fertilization and eyed egg rates were similar among the farms, hatching, swim‐up fry, and survival rates at the 120th day after hatching differed significantly. The average eyed egg, hatching and survival rates (calculated from selected 20 females) were 72, 55, and 32%, respectively. While sperm characteristics except duration of motility (s), were significantly different among the farms, average sperm concentration (per ml), motility (%) and duration of motility (s) in the region were 11.8 × 109, 55.3, and 56.4, respectively. The best hatchery performance was observed in Farm A because of better records and management applications than in the other farms.
      PubDate: 2016-03-24T02:41:37.161486-05:
      DOI: 10.1111/jai.13063
  • Length–weight relationships of Dermogenys pusilla Kuhl & van
           Hasselt, 1823 (Zenarchopteridae) and Labeo bata (Hamilton, 1822)
           (Cyprinidae) from the Ganges River (NW Bangladesh)
    • Authors: M. Y. Hossain; M. A. Hossen, M. N. U. Pramanik, K. Yahya, A. H. Bahkali, A. M. Elgorban
      Abstract: Length‐weight relationships (LWRs) were determined for Dermogenys pusilla (n = 75) and Labeo bata (n = 304) from the Ganges River, northwestern Bangladesh, collected between July 2013 and June 2014, using traditional fishing gear (e.g. cast net, square lift net and gill net). Total length (TL) was measured to 0.1 cm and whole body weight (BW) was taken to the nearest 0.1 g for each individual. The TL varied from 6.60 to 16.10 cm for D. pusilla and 7.90–25.20 cm for L. bata. The BW ranged from 1.20 to 10.90 g for D. pusilla and 4.70–167.30 g for L. bata. All LWRs were highly significant (P 
      PubDate: 2016-03-24T02:41:15.247115-05:
      DOI: 10.1111/jai.13070
  • Twenty‐four novel microsatellites for the endangered Chinese
           sturgeon (Acipenser sinensis Gray, 1835)
    • Authors: M.M. Xin; S.H. Zhang, D.Q. Wang, C.J. Li, H.M. Yue, Q.W. Wei
      Abstract: The Chinese sturgeon (Acipenser sinensis) is an endemic and critically endangered species in China. In this study, a total of 24 polymorphic microsatellite loci were isolated and characterized using Illumina sequencing for A. sinensis. The number of alleles (Na) per locus ranged from 2 to 6 (mean 4.04), mean expected heterozygosities (He), Shannon‐Wiener Diversity Indices (SW) and evenness (E) per locus ranged from 0.235 to 0.786 (mean 0.62), from 0.396 to 1.608 (mean 1.13), and from 0.060 to 0.213 (mean 0.13), respectively. Exact tests revealed that nine loci showed significant (P 
      PubDate: 2016-03-24T02:40:56.460214-05:
      DOI: 10.1111/jai.13071
  • Feeding ecology of a bagrid catfish, Mystus tengara (Hamilton, 1822) in
           the Tanore wetland of Rajshahi, Northwestern Bangladesh
    • Authors: N. R. Mitu; M. M. Alam
      Abstract: The feeding ecology of Mystus tengara (Hamilton, 1822) was studied in the Tanore wetland of Rajshahi, northwestern Bangladesh from January 2011 to December 2011. In fish purchased from the local market, a total of 14 713 food items were identified in the stomach contents of 3191 M. tengara specimens, an omnivorous catfish with a diversified feeding strategy including cladocerans, copepods, rotifers, gastropods, green algae, diatoms, insects, ‘small fish remains, fish fry and eggs’ and ‘other’ food items. Insects were the most dominant group of prey throughout the year. Seasonal changes in diet composition were due to the availability of prey items and fish size. Ontogenetic changes were noted between the juveniles and adults. The gut contents of M. tengara showed that the food items were from different layers of the water bodies.
      PubDate: 2016-03-23T01:52:13.831015-05:
      DOI: 10.1111/jai.13049
  • Morphology and parentage association of shortened upper jaw deformity in
           hatchery‐produced Japanese flounder, Paralichthys olivaceus
           (Temminck & Schlegel, 1846)
    • Authors: E. Sawayama; M. Takagi
      PubDate: 2016-03-23T01:51:50.188771-05:
      DOI: 10.1111/jai.13056
  • A new approach to quantifying setline angler effort and catch
    • Authors: B. L. Eder; M. A. Pegg, G. E. Mestl
      PubDate: 2016-03-23T01:51:27.587968-05:
      DOI: 10.1111/jai.13057
  • Length–weight relationships and length–length relationships of
           13 fish species in Rongcheng Bay, China
    • Authors: L. J. Wang; Z. H. Wu, M. M. Nie, M. X. Liu, W. Liu, F. You
      Abstract: The present study provides the length–weight relationships (LWRs) of 13 fish species belonging to eight families that were collected in Rongcheng Bay, March 2009 to December 2014. The r2 values ranged from 0.953 to 0.994 and values of b varied from 2.542 to 3.454. The study also describes the total length – standard length relationships (LLRs) for these fish species. This is the first record of length–weight and length–length relationships for the fish species in this area.
      PubDate: 2016-03-23T01:51:07.475402-05:
      DOI: 10.1111/jai.13058
  • Critical thermal maxima of juvenile alligator gar (Atractosteus spatula,
           Lacépède, 1803) from three Mississippi‐drainage
           populations acclimated to three temperatures
    • Authors: A. V. Fernando; S. E. Lochmann, A. H. Haukenes
      Abstract: Local adaptation may cause thermal tolerance to vary between nearby but distinct populations of a species. During the summer of 2013, alligator gar Atractosteus spatula spawned from broodstock collected from three populations within the Mississippi River drainage separated by a 5° latitudinal gradient were acclimated to three temperatures (25, 30, and 35°C). Ten fish from each population were acclimated at each temperature. CTMax was determined at each temperature for each population, using five fish for each population‐acclimation temperature pairing. CTMax for each population‐acclimation temperature pairing was compared using two‐factor anova. CTMax increased significantly with acclimation temperature (F2,40 = 600.5, P 
      PubDate: 2016-03-22T00:40:43.497427-05:
      DOI: 10.1111/jai.13047
  • Population genetics of Atlantic bluefin tuna, Thunnus thynnus (Linnaeus,
           1758), in the Mediterranean: implications for its conservation
    • Abstract: The movement of Atlantic bluefin tuna (ABFT) across international boundaries necessitates traceability strategies that would provide more accurate information needed for stock assessment. The Mediterranean Sea is one of the main contributors to ABFT reproduction and global population genetic diversity. In the present study this genetic variability was investigated using 193 samples of adult bluefin tuna from Spain, Turkey and Malta – a longitudinal distance of 3400 km. Analysed were 13 microsatellite loci (eight of which were newly‐tested) as genetic markers for the population study. Allele richness measured per locus and sampling location varied from 1.89 to 8.88, taking into account rarefaction. ABFT private alleles were detected in each of the three sampling sites. No significant spatial genetic divergence was found between pairs at the studied locations (FST values 0.05). Bayesian clustering analysis corroborated a single and panmictic ABFT population in the Mediterranean Sea. Statistical power analyses indicated a high probability of detecting genetic differentiation and population structure with the sample size and microsatellites used, even at an FST value of 0.005. From the results it may be postulated that migrating ABFT during the spawning season are allowing gene flow within the Mediterranean Sea. The complex interplay of movements, including plasticity in the selection of spawning sites with increasing age and environmental conditions, require multiple and new fisheries monitoring and management techniques in order to target the ABFT long‐term conservation effectively.
      PubDate: 2016-03-09T12:53:07.488433-05:
      DOI: 10.1111/jai.13035
  • Types and extent of fishing gear losses and their causes in the artisanal
           fisheries of Istanbul, Turkey
    • Abstract: Fishing gear losses remain a serious problem for marine life; these losses are also the source of an invisible fishing mortality. This study is an analysis of this problem within the artisanal fisheries of Istanbul. From September 2009 through May 2010, face‐to‐face interviews were conducted in 27 fishing ports for a total of 282 questionnaires. Estimates of lost fishing gear were 229.48 km of set nets, 2700 m of longlines, and 14 fish traps. Turbot nets had the highest loss ratio (54.73%), followed by bonito nets (16%), red mullet nets (7.36%), and encircling trammel nets (4.83%). The four major causes of net loss were: conflicts with other gear types (trawl and purse seine), conflicts with cargo vessels, bottom structure hindrances, and bad weather conditions. Bottom structures were the single factor in net losses for longlines and fish traps. The study observed that large quantities of nets had been lost, and that each net type showed a positive relationship between the number of nets used and the number of nets lost. Separate zones for artisanal and industrial fisheries are recommended as a realistic approach to reduce the losses of fishing gear and the impact of ‘ghost fishing’.
      PubDate: 2016-03-09T12:51:56.342024-05:
      DOI: 10.1111/jai.13046
  • A complete protocol for the preparation of chondrichthyan skeletal
    • Abstract: Summary The skeleton of cartilaginous fishes is notoriously difficult to prepare, and the literature dealing with the subject is extremely rare compared to other vertebrate groups. As a result, chondrichthyan skeletal specimens held in museums and other scientific institutions are often limited to isolated sets of jaws, taxidermised specimens and small individuals preserved whole in fluid. However, skeletal characters are heavily relied upon in several taxonomical and phylogenetical analyses, highlighting the importance of properly prepared skeletal specimens in research collections. The relevant literature is briefly reviewed herein, with a proposal for a new and simple protocol involving limited and easily obtainable chemicals, for the quick preparation of clean and durable chondrichthyan skeletal specimens. Various features of the chondrichthyan skeleton are also discussed, along with the microstructural effects of the chemicals involved in the protocol.
      PubDate: 2016-03-09T12:51:35.002088-05:
      DOI: 10.1111/jai.13050
  • Chemical and sensory quality changes in fish balls prepared from Alburnus
           mossulensis Heckel, 1843 during frozen storage
    • Authors: M. Duman; B. Peksezer
      Abstract: The effects of frozen storage on fish balls derived from Alburnus mossulensis at −18 ± 2°C were studied. Several chemical parameters were determined [pH, total volatile basic nitrogen (TVB‐N), thiobarbituric acid (TBA), free fatty acids (FFA)]. Further, microbiological effects were analysed during storage [Total mesophilic aerobic bacteria (TMAB), total psychrophilic bacteria (TPB), coliform and yeast‐mould count]. Finally, sensory quality tests were performed with fresh and stored balls. Fish balls were composed of 64% fish meat and 36% other ingredients, including: 18% boiled rice, 11.4% onions, 1.8% parsley, 3.8% fat, 0.7% salt and 0.26% black pepper. This basic mixture was processed to form small (25 g) balls and a pre‐cooking process was applied. Test balls were divided into two groups: vacuum packed (A1) and a control group without vacuum (A2). Test packages were stored for 6 months, resulting in increased pH, TVB‐N, TBA and FFA, while the values for sensory properties declined. However, these changes did not drop below the limit of acceptance. Total mesophilic aerobic bacteria and total psychrophilic bacteria were significantly reduced in both A1 and A2 groups.
      PubDate: 2016-03-09T12:51:07.157206-05:
      DOI: 10.1111/jai.13039
  • Effects of different baits on monofilament gillnet effectiveness in a
           freshwater reservoir fishery (Keban Dam Lake, Turkey)
    • Authors: M. Dartay; E. Duman
      Abstract: Aim of this study was to test the gillnet efficiency in Keban Dam Lake using different baits as attractants. Gillnets of 50 m length (stretched mesh sizes: 40, 50, 55 mm) were employed between January 2009 and December 2010 in the study. Two types of grain (corn and wheat) as well as processed plant material (beet cake) and animal livers were tested as bait to attract both carnivorous and omnivorous species. The catching efficiency of baited and non‐baited gill nets was compared. Overall, a total of 358 fish from eight different species were caught in baited gillnets, whereas only 189 fish were caught with non‐baited gillnets. Most fish (55.90%) were caught with corn, wheat or beet cake as bait; the fewest (59 fish, 9.96%) were captured with animal liver. About 34.1% were caught with non‐baited gillnets. By species, Capoeta trutta (Cyprinidae) was most frequently captured. In conclusion, the gillnets bated with corn, wheat, and beet cake yielded higher catches (P 
      PubDate: 2016-03-09T12:50:47.777952-05:
      DOI: 10.1111/jai.13038
  • Effects of clove oil, essential oil of Lippia alba and 2‐phe
           anaesthesia on juvenile meagre, Argyrosomus regius (Asso, 1801)
    • Abstract: The objectives of this experiment were to (i) determine the efficacy of essential oils of clove (CO) and Lippia alba (EOLA) to induce deep anaesthesia in juvenile specimens (49.0 ± 6.2 g body mass, 16.6 ± 0.8 cm; n = 8 per treatment) of meagre (Argyrosomus regius); and (ii) study the feasibility of these substances, together with 2‐phenoxyethanol (2‐PHE), as potential sedatives [low concentration: (i) EOLA: 12 mg L−1; (ii) CO: 1 mg L−1; and (iii) 2‐PHE: 33 mg·L −1; n = 8 per treatment] for live fish transport of this species. All test were performed at a constant temperature (18°C). Thus, the main primary stress indicator (plasma cortisol) and secondary factors (plasma metabolites) were evaluated. In addition, growth hormone (GH) mRNA expression was also evaluated in the pituitary gland. The results indicated that EOLA is considered to be effective for deep anaesthesia when the concentration is close to 160 mg L−1, while CO produces the same effect when lower concentrations are added (40–50 mg L−1). Regarding sedative concentrations, a significant ~3‐fold increase in plasma cortisol levels was detected in the EOLA group when compared to control specimens. In addition, glucose levels were not reduced and significantly increased (~1.6‐fold) for 2‐PHE in relation to the control fish. None of the anaesthetics promoted a significant difference for GH expression with respect to the control group, but a significant ~2‐fold increase for 2‐PHE treatment with respect to the EOLA exposition was found in this gene expression. Results show that none of the anaesthetics analysed, at least in the ranges of concentrations used in this study (EOLA 12 mg L−1, CO 1 mg L−1, 2‐PHE 33 mg L−1), are recommended for live fish transport, as shown by the absence of inhibition on the stress parameters assessed.
      PubDate: 2016-03-09T12:50:27.203819-05:
      DOI: 10.1111/jai.13048
  • Glutamine supplementation improves frozen‐thawed beluga sturgeon,
           Huso huso (Linnaeus, 1758) semen
    • Authors: M. S. Aramli; R. M. Nazari
      PubDate: 2016-03-09T12:50:20.911601-05:
      DOI: 10.1111/jai.13052
  • A comparison of fatty acid, cholesterol and vitamin composition in sea
           bass [Dicentrarchus labrax (Linnaeus, 1758)] and sea bream [Sparus aurata
           (Linnaeus, 1758)] from three cage farm areas: Antalya and Muğla
           (Turkey) and İskele (Northern Cyprus)
    • Abstract: In the present study the fatty acids, cholesterol and vitamin composition in farmed sea bass (8 fish per species per farm; weight range: 389.6–395.8 g, total length range: 297–316 mm) and sea bream (8 fish per species per farm; weight range: 386.8–391.7 g, total length range: 263–268 mm) from three cage farms (İskele in northern Cyprus, Antalya and Muğla in Turkey) were compared during the harvesting period in June–July 2011. The results showed that the muscles of D. labrax and S. aurata farmed fish were rich in n‐3 fatty acids, but with important differences. For example, the muscles of sea bass farmed in İskele were rich in docosahexaenoic acid (DHA) and n‐3 polyunsaturated fatty acids (PUFA). Palmitic acid (C16:0) was the primary saturated fatty acid, and oleic acid (C18:1 n‐9) the primary monounsaturated fatty acid in the muscle and liver samples of the cage‐farmed sea bass and sea bream. There were no significant differences in the cholesterol content in the muscles of sea bream farmed in İskele, Antalya or Muğla. In conclusion, the n‐3/n‐6 ratio in the muscle of farmed S. aurata and D. labrax is within the recommended limits for a healthy human diet, being very suitable for human nutrition.
      PubDate: 2016-03-08T00:43:33.994187-05:
      DOI: 10.1111/jai.13036
  • Tocopherol, heavy metals (Cd, Pb, Cu, Fe, Mn, Zn), and fatty acid contents
           of thornback ray (Raja clavata Linnaeus, 1758) liver oil in relation to
           gender and origin in the Mediterranean and Black seas)
    • Authors: A. Ozyilmaz
      Abstract: The study objective was to investigate the profiles of tocopherol, heavy metals, and fatty acids in the liver oil of female and male thornback ray Raja clavata from the Black and Mediterranean seas. The fish liver oil analyses were carried out by using a spectrophotometer (Hitachi U‐1900), ICP‐AES (Inductively Coupled Plasma‐Atomic Emission Spectrometry, and a GC‐MS (gas chromatography‐mass spectrometry), respectively. Results showed that the tocopherol content differed between the sample origin and gender (range of 42.3–134.5 mg kg−1). Pb, Cd, and Mn contents in livers of all R. clavata were lower than for Cu, Fe, and Zn. Levels of 7.6 and 8.9% in the eicosapentaenoic acid (EPA) were found to be higher in Black Sea thornback rays than in Mediterranean specimens (4.5 and 5.8%) (P 
      PubDate: 2016-03-08T00:43:14.983741-05:
      DOI: 10.1111/jai.13041
  • Growth and life history traits of Aegean chub, Squalius fellowesii
           (Günther, 1868) in streams in Muğla Province, Aegean coast,
    • Abstract: To aid in species' conservation, the aim of this study was to provide initial findings on age, growth and reproduction of an endemic species, Aegean chub Squalius fellowesii (Günther, 1868) populations from streams in the Aegean region of Muğla Province, Turkey. The species is relatively short‐lived (maximum 6 years), attaining a size of about 200 mm total length with a rapid growth to first maturity (≈60 mm TL), and relatively little growth thereafter. The male:female ratio was 1.0 : 0.6, males significantly outnumbering females in the majority of the streams. General condition values of individual fish varied between 2.9 and 3.4. Sexual maturity was usually achieved later and at larger sizes in females than in males. Sexual maturation in most populations was at the age of 2 years in females and 1 year in males. The species spawns between early April and late May. Mean absolute and relative fecundity were about 4440 eggs and 57 eggs·g−1, respectively. Mean egg diameter was 1.00 ± 0.03 mm, ranging from 0.70 to 1.20 mm. Suggestions for the conservation of Aegean chub are discussed.
      PubDate: 2016-03-08T00:42:53.866632-05:
      DOI: 10.1111/jai.13040
  • Ovarian development of albacore (Thunnus alalunga Bonnaterre, 1788) in the
           Eastern Mediterranean Sea: a histological characterization
    • Authors: F. S. Karakulak; T. Akayli, I. K. Oray, R. E. Yardimci
      Abstract: In order to investigate the unclear status of albacore (Thunnus alalunga) reproduction in the Eastern Mediterranean Sea, the stages of oogenesis from 129 females (64.0 and 83.5 cm fork length) were collected and analysed between April and August for the years 2002 and 2008. Fish were captured by commercial vessel gillnets. Based on histological examination of the ovaries, a total of seven ovarian developmental stages were identified: (i) perinucleolar, (ii) phase of early lipid enrichment, (iii) early vitellogenesis, (iv) late vitellogenesis, (v) migratory nucleus, (vi) pre‐hydrated and (vii) hydrated oocyte phase. Data on the monthly gonadosomatic index and the results of the histological analyses indicate that in the region the female T. alalunga mature in May. The spawning stage is predominant in June, reaching a maximum in July. The evidence reported here indicates that size at first sexual maturity for females was lower for the Mediterranean albacore stock than for the Pacific and Atlantic stocks.
      PubDate: 2016-03-06T23:22:25.492636-05:
      DOI: 10.1111/jai.13034
  • Effect of absorbent pads containing black seed or rosemary oils on the
           shelf life of sardine [Sardina pilchardus (Walbaum, 1792)] fillets
    • Authors: B. Kilinc; S. Altas
      Abstract: The effects of essential oils on the shelf life of sardine (Sardina pilchardus) fillets in cold storage were assessed at 4–6°C. The oils were sprayed on the fillets as well as with the addition of absorbent food pads that also contained essential oils. Sardine fillets were divided into eight test groups. Group A: fillets stored without treatment (control group); Group B: fillets packaged with food pads (without the addition of essential oils); Group C: Rosemary oil sprayed onto food pads (10 ml of 1.5% RO); Group D: Black seed oil sprayed onto the food pads (10 ml of 1.5%BSO); Group E: Rosemary oil sprayed onto both sides of the sardine fillets (10 ml of 1.5%RO); Group F: Black seed oil sprayed onto both sides of sardine fillets (10 ml of 1.5% BO); Group G: Rosemary oil sprayed onto both sides of the sardine fillets (10 ml of 1.5% RO) and on the food pads (+10 ml of 1.5% RO); and Group H: Black seed oil sprayed onto the sardine fillets and the food pads (10 ml of 1.5% BSO onto both sides of fillets +10 ml of 1.5% BSO onto food pads). Tests were carried out at days 0, 3, 5 and 7. When compared to the control group, food pads containing rosemary and black seed oils extended the shelf life of sardine fillets by approximately 2 days at 4–6°C. Group A and B exceeded the limit of consumption on day 3, and Group C and D exceeded this microbiological limit after day 5. RO and BSO both exhibited the same antimicrobial effects on the shelf life of sardine fillets. Groups E, F, G and H prolonged the microbiological threshold by 7 days compared to the control. However, Group G and H had 1–1.5 log lower TVC load than Group E and F. Food pads containing antimicrobial essential oils may be used to extend the shelf life of fresh fish, however, further investigations are needed to determine safety standards.
      PubDate: 2016-03-06T23:20:11.18852-05:0
      DOI: 10.1111/jai.13044
  • Morphological and morphometric evaluation of silver barb, Barbodes
           gonionotus (Bleeker, 1849) sperm supplemented with antibiotics
    • Authors: T. Boonthai; W. Khaopong, J. Sangsong, S. Nimrat, V. Vuthiphandchai
      Abstract: Aim of this study was to evaluate sperm morphology of silver barb, Barbodes gonionotus, sperm and describe the effect of antibiotics on morphological characteristics of the sperm using an ASMA plug‐in. The experiment was done at the room temperature (25°C) and divided into four treatments in three replicates: (i) freshly collected semen, (ii) extended semen (control), (iii) extended semen supplemented with 0.5% penicillin‐streptomycin (PS), and (iv) extended semen supplemented with 0.5% penicillin‐gentamicin (PG). Silver barb sperm comprised three main compartments: a circular head with no acrosome, a midpiece, and a single flagellum. Addition of 0.5% PS had no detrimental effects on sperm morphometry, except flagellum width. Administration of 0.5% PG affected sperm morphology in two distinct ways: (i) intact sperm (76.92 ± 5.84% of total sperm) except for flagellum width, and (ii) severe morphological damage (23.08 ± 2.67%).
      PubDate: 2016-02-24T05:15:12.99391-05:0
      DOI: 10.1111/jai.12960
  • Length–weight relationships of six fish species from the Lancang
           River, China
    • Authors: H. Y. Sun; J. L. Yao, Y. Cheng, Y. F. Chen
      Abstract: Length–weight relationships of six fish species are presented. Fish samples were collected from the Lancang River, in the Chinese section of the Upper Mekong River, in southwestern China. This study reports the first length–weight relationship for one of these species.
      PubDate: 2016-02-22T05:14:47.46558-05:0
      DOI: 10.1111/jai.13007
  • Feeding habits of the Pacific bearded brotula Brotula clarkae Hubbs, 1944
           (Ophidiidae) along the Pacific coast of Costa Rica, Central America
    • Abstract: The present study analyzed the diet composition, ontogenetic shifts and dietary overlap of Brotula clarkae in relation to stage of maturity and sex. Samples were collected from the trawling fishery along the Pacific coast of Costa Rica (2011–2012) at depths ranging from 41.4 to 168.3 m; however, over 80% of the sampled fish were obtained at depths between 50 and 75 m. Size ranged from 14.4 to 98.4 cm total length. Of the 323 analyzed stomachs, 44.3% were from males, 86% were from immature individuals, and 49.8% had at least one prey item. According to the prey‐specific index of relative importance (PSIRI), decapod shrimps were the most important prey (57.6% PSIRIi) followed by teleosts (28.2% PSIRIi), stomatopods (10.8% PSIRIi), and crabs (3.3% PSIRIi). Male and female B. clarkae exhibited a high dietary overlap (CH = 0.94). Immature B. clarkae consumed primarily shrimps and crabs (71.5% of stomachs from immature specimens contained shrimps, which accounted for over 66.0% PSIRIi); mature individuals consumed a large proportion of teleosts and stomatopods, which together contributed to over 91.0% PSIRIi. Both immature and mature B. clarkae overlapped spatially with the commercial trawling fishery grounds along the Pacific coast of Costa Rica. However, juveniles feed predominantly on shrimps, suggesting that immature B. clarkae may be subjected to high fishing pressure as by‐catch, making them particularly vulnerable to overexploitation.
      PubDate: 2016-02-11T03:17:54.929453-05:
      DOI: 10.1111/jai.13029
  • Length‐weight relationships of five fish species from the Yalong and
           Wujiang rivers (tributaries of Yangtze River, China)
    • Authors: L. Pan; S. R. Yang, L. Wan, Z. Yang, Z. Li, Y. Qiao
      Abstract: Length‐weight relationships (LWRs) were determined for five endemic fish species from the Yalong and Wujiang rivers (tributaries of the Yangtze River, China): Folifer brevifilis (Peters, 1881), Pelteobagrus ussuriensis (Dybowski, 1872), Schistura fasciolata (Nichols & Pope, 1927), Triplophysa daqiaoensis (Ding, 1993) and Triplophysa orientalis (Herzenstein, 1888). Samples were obtained between April 2004 and July 2014 using various fishing gear (set nets, drift gill nets, fish cages, hook and electro fishing). For each specimen from each species the sample size was recorded, total length and weight were measured and the LWR determined. Further, the 95% confidence intervals of a and b, and coefficient of correlation were estimated. Prior to this study, the LWRs for these five species were unknown.
      PubDate: 2016-02-06T01:06:32.461221-05:
      DOI: 10.1111/jai.13000
  • Length–weight relationships of three fish species from the Jinsha
           River, southwestern China
    • Authors: C. X. Xia; L. Chen, W. Xiong
      Abstract: Length–weight relationships were determined for the first time for three fish species (Xenocypris fangi Tchang, 1930; Microphysogobio kiatingensis Wu, 1930; and Opsariichthys bidens Günther, 1873) from the Jinsha River, southwestern China. Samples were collected using gillnets (30 × 15 m, mesh‐size 5 mm) and electroshock fishing techniques (CWB‐2000 P, 12 V, 250 Hz).
      PubDate: 2016-02-04T04:35:18.410155-05:
      DOI: 10.1111/jai.13014
  • Molecular phylogeny of three mudskippers (Gobiidae) from the Persian Gulf
           and Gulf of Oman (Makran)
    • Authors: M. Ghanbarifardi; H. R. Esmaeili, Z. Gholami, M. Aliabadian, B. Reichenbacher
      Abstract: Presented is a phylogenetic reconstruction of mudskippers using sequences of the mitochondrial COI and cytb genes. The phylogeny combines the sequences of 12 species from GenBank with those of three species (Boleophthalmus dussumieri, Periophthalmus waltoni and Scartelaos tenuis) collected from the coastal area of the Persian Gulf and Oman (Makran) Sea and sequenced for the first time. Based on the Maximum likelihood and Bayesian methods, Periophthalmus forms a separate clade that is the sister lineage to a group formed by all remaining genera. The subfamilies Oxudercinae and Amblyopinae established on the basis of morphological characters have been shown to be paraphyletic and their use as taxonomic units is no longer recommended. The evolution of Periophthalmus waltoni, Periophthalmus barbarous and Boleophthalmus dussumieri is discussed based on molecular phylogenetic reconstruction.
      PubDate: 2016-02-02T02:25:28.966596-05:
      DOI: 10.1111/jai.12999
  • Discard reduction of trammel nets in the Northeastern Mediterranean prawn
    • Abstract: In this study the effects on the discard reduction of guarding net rigged prawn trammel nets were examined with the focus on catch composition and income of fishermen for the Mersin Bay small‐scale prawn fishery. A total of 15 fishing trips were carried out between September and December 2011, using the commercial net and the alternative experimental net for comparison. The results revealed that catches of the main target species, green tiger prawn (Penaeus semisulcatus), in the alternative nets were only 1.5% smaller than those of the commercial nets, and with a statistically insignificant difference (P > 0.05). However, the catch of the main discarded species, the invasive swimming crab (Charybdes longicollis), mantis shrimp (Rissoides desmaresti), and the blue crab (Portunus pelagicus) were captured 85%, 66%, 75% less often in the alternative nets, with statistically significant differences (P 
      PubDate: 2016-02-02T02:25:08.546008-05:
      DOI: 10.1111/jai.13015
  • Species composition of benthic fish fauna in the Sea of Marmara, Turkey
    • Abstract: In order to determine the present benthic fish fauna in the Sea of Marmara, sampling was conducted at a total of 10 stations for 2 weeks in August 2009, using a commercial twin beam trawling vessel with 18 and 36 mm stretched mesh sizes at the cod‐end. In addition, some physicochemical parameters that play important roles, such as temperature, salinity, dissolved oxygen, and pH, were measured for each station. As a result, 31 fish species from 23 families were found at a total of 10 stations in depths of 30–1000 m. At each station, the Shannon–Weaver diversity index, Simpson dominance index, species richness index, and evenness index were estimated. This study reports on species composition of the demersal fish catches in the Sea of Marmara for a preliminary assessment of the status of these fish communities.
  • A checklist of the protozoan and metazoan parasites of European eel
           (Anguilla anguilla): checklist of Anguilla anguilla parasites
    • Abstract: A comprehensive literature review on prevalence and distribution of parasites of the European eel (Anguilla anguilla) in European waters was conducted to set a baseline for future trend analyses and biodiversity considerations. The resulting checklist compiled from peer reviewed journals as well as grey literature including scientific reports, conference contributions, PhD theses and own survey data includes all protozoan and metazoan parasites of the European eel from freshwater, brackish and marine habitats. Parasites are arranged according to phylum and family. Species within families are listed alphabetically. Infected host organs, recorded habitats, localities, countries and literature citations are provided for each species. A total of 161 parasite species/taxa from 30 countries are listed. With the trypanorhynch cestode Tentacularia coryphaenae a new host record could be documented. Furthermore, three new locality records of parasitic nematodes could be added based on own survey data: Anisakis simplex from an eel caught in the Baltic Sea, Eustrongylides mergorum for German freshwaters, and Goezia anguillae from the Tagus estuary in Portugal. Special consideration was given to the distribution of the swimbladder nematode Anguillcoloides (Anguillicola) crassus. A map and table of first reported records of this neozoan parasite species for 25 countries as well as a survey map indicating all published localities documented in the checklist are provided.
  • Inner Title Page
  • Foreword
  • Morphometric differentiation between two juvenile tuna species [Thunnus
           thynnus (Linnaeus, 1758) and Euthynnus alletteratus (Rafinesque, 1810)]
           from the Eastern Mediterranean Sea
    • Abstract: The main objective of this study was to analyse the differences in morphometric characteristics among specimens of Atlantic juvenile bluefin tuna Thunnus thynnus [12.2–46.5 cm fork length (FL)] and juvenile little tuna Euthynnus alletteratus (14.1–26.4 cm FL). A total of 353 bluefin tuna (young of the year) and 288 little tuna (young of the year) were collected from the commercial hand line fisheries in the eastern Mediterranean Sea between July and October of 2011–2013 with three round‐bent hook sizes (numbers 10, 12 and 14; Mustad 2315S). By using univariate and multivariate analysis, 11 morphometric characters were investigated. anova revealed highly significant differences (P 
  • Length–weight relationships of seven fish species from the upper
           Pelus River, Kuala Kangsar district, Perak, Malaysia
    • Abstract: Length–weight relationships (LWR) are reported for seven fish species from the upper Pelus River, Kuala Kangsar district, Perak, Malaysia. This river is located in a remote area and supplies fishes for the aboriginal peoples. Fish samplings were conducted in three different seasons, the dry (June 2014), wet (August 2014), and moderately wet (April 2015). All samples were collected using fish electro–shocker and scoop nets with a 1 cm mesh size. A total of seven LWRs were unknown to FishBase, and four new maximum fish lengths were identified in the study.
  • Length–weight relationships of four freshwater cyprinid species from
           a tributary of Ganga River Basin in North India
    • Abstract: This paper provides length‐weight relationships (LWRs) for four freshwater cyprinid fish species, namely Barilius bendelisis (Hamilton, 1807), Bangana dero (Hamilton, 1807), Chagunius chagunio (Hamilton, 1807) and Labeo dyocheilus (McClelland, 1839) from the Kosi River, a tributary of the River Ganga in northern India. Altogether 848 specimens were caught bimonthly (for B. bendelisis) and quarterly for the other three species between March 2013 and December 2014 using cast nets (9 m length, 9 m width and 1/2 cm mesh size). LWRs for two of the four species (B. dero and C. chagunio) are recorded for the first time. The present study results will be useful for fishery researchers, management and conservation in this least‐explored tributary of the Ganga River Basin.
  • Length–weight relationships for common juvenile fishes and prey
           species in Hawaiian estuaries
    • Abstract: Length–weight relationships (LWR) using 3876 individuals were determined for 39 species sampled in estuarine systems in the main Hawaiian Islands, including 33 species of fish from 21 families, five species of crustaceans (prey) from two families, and one mollusk species. For 17 species, LWRs were estimated for the first time. For another 14 species their LWRs were newly reported for Hawaiian waters, including seven introduced taxa that are well established in the islands' estuaries. The LWRs for all taxa include juvenile and sub‐adult size ranges commonly encountered in Hawaiian estuaries.
  • Length–weight relationships of four indigenous freshwater fish
           species from Khalsi wetland in lower Ganga basin, West Bengal, India
    • Abstract: The length‐weight relationships of four indigenous freshwater fishes Parambassis lala (Hamilton, 1822), Trichogaster lalius (Hamilton, 1822), Puntius terio (Hamilton, 1822), and Pethia phutunio (Hamilton, 1822) were determined. Fish samples were collected during December 2013 to May 2015 from Khalsi, a floodplain wetland along the tributary of the River Ganges in West Bengal state, India using different types of gear (castnets, gillnets and drag nets, stretched mesh sizes of 15–20 mm, 20–30 mm and 5–10 mm, respectively). This is the first report on the length–weight relationship parameters of these four species.
  • Length–weight and length–length relationships of three
           cyprinid fishes from the Bibi‐Sayyedan River, western Iran
    • Abstract: This study describes the length–weight and length–length relationships for three cyprinid species from the Bibi‐Sayyedan River (western Isfahan provinces, Iran). The slope parameter (b) values in the length–weight relationship equations were determined as 3.0729 for Alburnus mossulensis Heckel, 1843; 2.8509 for Barbus lacerta Heckel, 1843; and 3.0864 for Chondrostoma regium (Heckel, 1843). This study presents the first LWR and LLR references for these species in the Bibi‐Sayyedan River.
  • Issue Information
  • Length–weight and length–length relationships for three
           endemic Pseudophoxinus species in Turkey
    • Abstract: Length–weight and length–length relationships are presented for three endemic Pseudophoxinus species in Turkey. This study provides the first references for these relationships in the species examined.
  • Eumetazoan parasites of two marine fish species from Baja California,
           Mexico: Sebastes miniatus (Jordan & Gilbert, 1880) and Caulolatilus
           princeps (Jenyns, 1840)
    • Abstract: It is hypothesized that two fish species sharing a same demersal habitat might show a similar community of parasites. The aim of this study was to compare the parasite community component of of two marine fishes on the northwest coast of México of economical interest, the vermilion rockfish Sebastes miniatus and the ocean whitefish Caulolatilus princeps. From February to December 2005, 210 specimens of S. miniatus (85–550 mm total length TL) and 91 C. princeps (85–550 mm TL) were obtained from the San Quintín sportfishing catch, at Pacific Baja California. Skin, eyes, fins and gill cavity were examined in situ, and their internal organs removed for later analysis, including a portion of flesh. In S. miniatus, 12 parasite taxa were identified as belonging to Monogenea, Digenea, Cestoda, Nematoda, Acanthocephala and Copepoda; the stomach, pyloric caeca, and intestine (digestive tract) were the most parasitized organs with seven of the 12 species. In S. miniatus, the most abundant parasite was Parabothriocephalus sagitticeps (80.8 individuals/host ± 33.4 SE: standard error), highest prevalence was Microcotyle sebastis (92.6%), and the highest average intensity of infection was the cestode P. sagitticeps (190.4 ± 48.1 SE). The C. princeps showed six species belonging to Monogenea, Digenea, Nematoda and Copepoda; the digestive tract presented the greatest infestation, with four of the six species recorded. The most abundant nematode was Hysterothylacium aduncum (16.5 ± 3.5 individuals/host), the main prevalent species was Anisakis sp., and the highest value of infection intensity was by Hatschekia sp. The nematodes Anisakis sp. and H. aduncum were shared between the two host fishes, and are a zoonotic risk to humans if the fishes are not cooked properly.
  • Isolation and evaluation of autochthonous Bacillus subtilis strains as
           probiotics for fat snook (Centropomus parallelus Poey, 1860)
    • Abstract: The objective of this study was to isolate and select autochthonous strains of Bacillus subtilis from the fat snook, Centropomus parallelus, and examine the viability of the Bacillus bacteria to determine their beneficial effect on gut colonization in reared fish. Twenty strains of Bacillus were isolated and further confirmed as B. subtilis using PCR. Among the 20 strains, two strains (B02 and B03) exhibited an inhibitory performance against five tested pathogens. The Bacillus strains B02 and B03 were added to the fish ration, and after 30 days the number of viable colonies were maintained or increased under the conditions of refrigeration (5°C), freezing (−18°C), or room temperature (30°C). These strains showed a growth rate of 0.18–0.21 h and a doubling time of 3.34–3.9 h. Both strains were tolerant to variations in NaCl, and B03 was also tolerant to bile exposure. The ability to colonize the gastrointestinal tract was also examined in healthy fat snook juveniles fed diets enriched for 30 days with strains B02 and B03. The amount of B. subtilis in the gastrointestinal juvenile tract was significantly higher in fish fed the enriched ration compared to controls. Based on these results, strains B02 and B03 were considered as candidate probiotics for fat snook.
  • Length–weight relationships of nine freshwater fish species from the
           Liujiang River, Guangxi, China
    • Abstract: Studied were the length–weight relationships (LWRs) of 599 fish specimens covering four families, nine genera and nine species (Sinibrama macrops, Acrossocheilus parallens, Microphysogobio fukiensis, Squalidus wolterstorffi, Pseudohemiculter dispar, Tachysurus fulvidraco, Hemibagrus macropterus, Pseudobagrus crassilabris and Siniperca kneri) collected in the Liujiang River, a tributary of the Pearl River, in March 2014, September 2014 and March 2015. The b value varied between 2.385 (P. crassilabris) and 3.178 (P. dispar), with the mean b = 2.85 at P 
  • Testing staining techniques to determine age and growth of Dasyatis
           pastinaca (Linnaeus, 1758) captured in Iskenderun Bay, northeastern
    • Abstract: This study tested the suitability of several staining methods to determine the age of common stingray (Dasyatis pastinaca) from Iskenderun Bay, Turkey. A total of 384 specimens (16.6 cm–69.3 cm disc width) were obtained by trawling between September 2010 and December 2011. Sex ratio of the samples was 53% males and 47% females. Appropriate age determination was firstly demonstrated using Safranin‐O staining. Age readings were made by two independent readers and the index of average percent error (IAPE) determined as 6.3% for Safranin‐O, 6.8% for Crystal Violet, 7.9% for Alcian Blue, and 9.3% for Silver Nitrate. Safranin‐O and Crystal Violet staining methods provided the best results. Verification of temporal growth ring formation was by marginal increment analysis. Disc width–weight relationships were determined by W = 0.0272*DW3.06 for females and W = 0.0247*DW3.08 for males. Estimates of the von Bertalanffy growth parameters indicated a larger asymptotic disc width (DW∞ = 127.06 cm) for females than for males (DW∞ = 114.54 cm); growth parameters were k = 0.058 year−1, to = −1.508 and k = 0.041 year−1, to = −3.632 for females and males, respectively.
  • Estimation of genetic parameters for disease‐resistance traits in
           Cynoglossus semilaevis (Günther, 1873)
    • Abstract: The objective of this study was to estimate genetic parameters for three traits based on 28 Cynoglossus semilaevis families approximately 6 months of age (at least 5 cm total length), including trait_1 (survival of 26 families, 3434 individuals in total subjected to challenge tests with Edwardsiella tarda), trait_2 (survival of 20 families, 2016 individuals in total subjected to challenge tests with Vibrio anguillarum) and trait_3 (survival of 27 families, 9340 individuals tagged at circa 180 days of age and reared in indoor ponds for circa another 5 months). The result showed that there were large differences in the survival of the families after challenge (11.11–65.31% for E. tarda and 9.18–70.54% for V. anguillarum). Additionally, the survival of families reared in indoor ponds was also different, varying from 21.00% to 73.67%. Heritabilities of the three traits varied from 0.14 to 0.26, as estimated by the linear model (LM) and the threshold model (TM). The trait_1 heritabilities (0.26 and 0.19 estimated by LM and TM) were higher than those of the others (0.20 and 0.23 estimated by LM, 0.14 and 0.19 estimated by TM). The estimates of heritabilities using LM were consistently higher than those of TM in this study. There were significant medium genetic correlations of 0.44 and 0.42 between trait_1 and trait_2 obtained from LM and TM (P 
  • Population structure, length–weight relationship and growth of white
           seabream, Diplodus sargus sargus (Linneaus, 1758), in Beymelek Lagoon,
    • Abstract: The main objective of this study was to investigate the population structure, length–weight relationship (LWR) and growth of white seabream, Diplodus sargus sargus (L., 1758) in Beymelek Lagoon, Turkey. Samples were collected by gill‐ and trammel nets of various mesh sizes, February 2006 to January 2007. A total of 355 specimens (51 males; 34 females; 270 unidentified) were collected. Male to female ratio was 1.50 : 1. Ages ranged from 0 to 3 years; about 70% were juveniles. Most fish were between 13 and 16 cm in total length and below 90 g in weight. The LWR of all individuals was W = 0.0140 × L3.1028. The von Bertalanffy growth equation was calculated as Lt = 39.9 × [1 – exp(−0.268 × (t + 1.753)] and the growth performance index (Φ′) was 6.056.
  • Length–weight relationships for 10 elasmobranch species from the
           Oman Sea
    • Abstract: Length–weight relationships (LWRs) were estimated for ten elasmobranch species from Iranian waters of the Oman Sea. All specimens were collected by bottom trawl (mesh size 80 mm in the cod end) between May and December 2014. A total of 509 individuals (107 Torpedo sinuspersici, 68 Rhinobatos punctifer, 63 Chaenogaleus macrostoma, 72 Himantura walga, 80 Himantura gerrardi, 58 Gymnura poecilura, 4 Himantura uarnak, 4 Rhinoptera javanica, 14 Aetobatus flagellum, and 39 Pastinachus sephen) were sampled and studied. Results showed that most species had positive allometric or isometric growth but that more studies were needed for final decisions on growth patterns. Both the disk length‐body weight (DL/BW) and disk width‐body weight (DW/BW) relationships also showed a good fit, reinforcing the credibility of the data in relation to the LWRs. This study is the first report of LWRs for these elasmobranchs in the northern Oman Sea.
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