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Journal Cover Journal of Applied Ichthyology
  [SJR: 0.482]   [H-I: 36]   [6 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0175-8659 - ISSN (Online) 1439-0426
   Published by John Wiley and Sons Homepage  [1597 journals]
  • Length‐weight relationships for five Clupeiformes species from the
           Persian Gulf and Oman Sea
    • Abstract: Length‐weight relationships (LWRs) were determined for five Clupeiformes species representing three families collected from the Persian Gulf and Oman Sea. These are the first references on length‐weight relationships for two of these species (Ilisha megaloptera and Sardinella sindensis). A new maximum length record was obtained for one species (Ilisha melastoma).
      PubDate: 2015-11-23T01:20:05.074828-05:
      DOI: 10.1111/jai.12962
  • Length–weight relationships of 13 fish species from Pahang River,
           Temerloh district, Pahang, Malaysia
    • Authors: A. R. Zulkafli; M. N. A. Amal, S. Shohaimi, A. Mustafa, A. H. Ghani, S. Ayub, S. Hashim, M. I. Anuar, M. P. Hasfairi
      Abstract: Length–weight relationships (LWRs) are described for 13 fish species from Pahang River, Temerloh district, Pahang, Malaysia. The river serves as a main water body for freshwater aquaculture in the country and also supplies various fishes for local consumption. This is the first record of LWRs for nine fish species and new maximum lengths for three species in fishbase.
      PubDate: 2015-11-23T01:20:02.079459-05:
      DOI: 10.1111/jai.12959
  • Length–weight and length–length relationships of seven fish
           species from the Ili River and tributaries, northwest China
    • Abstract: Length–weight and length–length relationships were estimated for seven fish species belonging to four families from the Ili River and its tributaries, northwest China. From April to October 2013 and May to October 2014, specimens were collected monthly for a total of 783 specimens. This study presents the first references on LWRs for Diptychus maculates, Gymnodiptychu dybowskii, Perca schrenkii, Silurus meridionalis and Schizothorax pseudoaksaiensis pseudoaksaiensis, and LLRs for G. dybowskii, P. schrenkii, S. meridionalis, S. pseudoaksaiensis pseudoaksaiensis, Triplophysa strauchii and Triplophysa stoliczkai.
      PubDate: 2015-11-23T01:19:20.736178-05:
      DOI: 10.1111/jai.12964
  • Effects of varying levels of dietary vitamin E (α‐tocopherol)
           on growth performance, proximate and fatty acid composition of juvenile
           silver pomfret (Pampus argenteus Euphrasen, 1788)
    • Authors: M. A. Hossain; S. M. Almatar, S. B. Yaseen
      Abstract: This study investigated the effects of elevated dietary levels of vitamin E (α‐tocopherol) on growth performance, proximate composition and fatty acid profiles of juvenile silver pomfret, Pampus argenteus. Three semi‐purified experimental diets were formulated to contain 49% protein and 16% lipid. High docosahexaenoic acid (DHA) tuna oil was added to the diets to supplement DHA. A graded level of vitamin E (0‐, 50‐, and 100 mg kg−1) was added to experimental diets 1 to 3, respectively. Analyzed vit. E levels were 155.2, 195.3 and 236.4 mg kg−1 in diets 1, 2 and 3, respectively. The experiment was conducted for 12 weeks with juvenile silver pomfret (29.6 ± 7.6 g) using a flow‐through system consisting of nine 1‐m3 tanks. Each treatment had three replicates and fish were stocked at the rate of 20 m−3. Growth performance and feed utilization parameters of fish fed diets 2 and 3 were significantly (P  0.05). Although whole body protein levels were not influenced by the dietary vit. E levels, whole body lipid in fish fed diet 2 was significantly higher than in fish fed the other diets. The whole body vit. E levels in fish fed diet 2 (22.6 mg kg−1) and diet 3 (24.1 mg kg−1) were significantly (P 
      PubDate: 2015-11-23T01:18:17.747168-05:
      DOI: 10.1111/jai.12956
  • Effects of visible implant elastomer and coded wire tags on growth and
           survival of Schizopygopsis younghusbandi Regan, 1905
    • Authors: T. B. Zhu; W. Guo, X. B. Wu, Y. F. He, D. G. Yang
      PubDate: 2015-11-23T01:16:36.619215-05:
      DOI: 10.1111/jai.12958
  • Issue Information
    • PubDate: 2015-11-20T02:20:59.79986-05:0
      DOI: 10.1111/jai.12578
  • Length–weight relationships of fifteen endemic freshwater fishes in
           South Korea
    • Abstract: This study estimated LWRs of fifteen Korean endemic fish species, which had no prior LWR data in fishbase. Fish samples were collected quarterly in the Korean Peninsula from 2010 to 2013 by cast net, gill nets and a kick net. Ranges of parameters a and b of the 15 species were from 0.00398 to 0.01141 and 0.96 to 3.32, respectively.
      PubDate: 2015-11-07T00:57:36.983957-05:
      DOI: 10.1111/jai.12943
  • First record of bigeye thresher shark (Alopias supercillosus Lowe, 1841)
           and new record of thresher shark [Alopias vulpinus (Bonnaterre, 1788)]
           (Chondrichthyes, Alopiidae) from Argentina
    • PubDate: 2015-11-07T00:56:55.657022-05:
      DOI: 10.1111/jai.12945
  • Isolation and characterization of 21 polymorphic microsatellite markers
           for a new labeonine fish (Paraqianlabeo lineatus Zhao et al. 2014)
           using illumina paired‐end sequencing
    • Authors: Y. Li; H. Zhao, Z. Peng, Y. Zhang
      PubDate: 2015-11-07T00:54:47.118283-05:
      DOI: 10.1111/jai.12938
  • Length–weight relationships of 15 fish species from Tembeling River,
           Pahang, Malaysia
    • Authors: A. R. Zulkafli; M. N. A. Amal, S. Shohaimi, A. Mustafa, A. H. Ghani, S. Hashim, M. I. Anuar, S. Ayub
      Abstract: This study reports the length–weight relationships (LWR) of 15 fish species from Tembeling River, Pahang, Malaysia. The river flows adjacent to the National Park, Pahang, and supplies fishes for local consumption. In addition, this river also functions as a main water body for protection and breeding of commercial fish species. A total of eight LWRs were unknown to FishBase, and five new maximum lengths were identified from the study.
      PubDate: 2015-11-07T00:53:58.641472-05:
      DOI: 10.1111/jai.12939
  • Diet of labrisomid blenny Auchenionchus variolosus (Valenciennes, 1836)
           (Labrisomidae) during its larval development off central Chile
    • Abstract: The diet, feeding success (prey number and total volume per gut, and maximum prey width) and trophic niche width of the labrisomid blenny Auchenionchus variolosus (Valenciennes, 1836) was studied during its larval development (3.93–17.26 mm standard length). Individuals were collected in October 2012 and October 2013 in nearshore waters (
      PubDate: 2015-11-07T00:53:05.834659-05:
      DOI: 10.1111/jai.12935
  • Length–weight relationships of three endemic fish species from the
           upper Yellow River, China
    • Abstract: Length–weight relationships (LWRs) of three Chinese endemic fish species (Gymnocypris eckloni eckloni Herzenstein, 1891, Platypharodon extremus Herzenstein, 1891 and Triplophysa pseudoscleroptera (Zhu & Wu, 1981)) were analyzed, collecting from the upper Yellow River (Xunhua and Guide Counties, Qinghai Province). Totally 347 specimens monthly collected by gill nets with 40 mm mesh size and traps with 5 m length from August to October 2014, were used to estimate the relationship parameters. The allometric factor b values ranged from 2.96 in T. pseudoscleroptera to 3.33 in G. eckloni eckloni. The condition factor a values varies between 0.0063 and 0.0175 in three species. Three WLRs of fishes distributed in the upper Yellow River were firstly reported and will fill in some of the empty spaces on endemic species in the Fishbase.
      PubDate: 2015-11-07T00:52:37.152411-05:
      DOI: 10.1111/jai.12928
  • Separating two herring stocks from growth data: long‐term changes in
           survey indices for Western Baltic Spring Spawning Herring (Clupea
           harengus) after application of a stock separation function
    • Abstract: In the Baltic Sea, herring stocks are surveyed and managed according to a spatial allocation based on ICES (International Council for the Exploration of the Sea) subdivisions. In the western Baltic, the distribution areas of two stocks overlap: the Western Baltic Spring Spawning Herring (WBSSH) and the Central Baltic Herring (CBH). Survey results of length‐at‐age data indicate in Subdivision (SD) 24, which is a part of the WBSSH management area, that a considerable fraction of CBH is present and correspondingly erroneously allocated to WBSSH stock indices. Accordingly, a stock Separation Function (SF) based on growth parameters was established to identify the fraction of CBH in the WBSSH area. In the present study, the SF was applied to 8 years of data from the GERman Acoustic Survey (GERAS), which is conducted annually in autumn in ICES subdivisions 21‐24. Results showed a distinct fraction of CBH in SD 24, and exclusion of the CBH greatly improved the quality of the GERAS index used in the assessment of the WBSSH stock.
      PubDate: 2015-11-04T03:25:44.141333-05:
      DOI: 10.1111/jai.12924
  • Updated assessment of hatchery‐reared pallid sturgeon (Forbes &
           Richardson, 1905) survival in the lower Missouri River
    • Authors: K. D. Steffensen; L. A. Powell, S. M. Stukel, K. R. Winders, W. J. Doyle
      Abstract: As pallid sturgeon, Scaphirhynchus albus (Forbes & Richardson, 1905), natural reproduction and recruitment remains very minimal in the lower Missouri River from Gavins Point Dam (river kilometer [rkm] 1305.2) to the confluence with the Mississippi River (rkm 0.0), hatchery supplementation and river‐wide monitoring efforts have continued. Annual survival estimates of hatchery‐reared pallid sturgeon stocked in the lower Missouri River were previously estimated during 1994–2008. Low recapture rates prior to 2006 limited the data available to estimate survival, which resulted in considerable uncertainty for the estimate of annual survival of age‐1 fish. Therefore, the objective was to provide more precise estimates of annual survival of pallid sturgeon using five additional years of stocking and sampling. The Cormack‐Jolly‐Seber model structure provided in program MARK was used to estimate the age‐specific survival estimates. Over 135 000 hatchery‐reared pallid sturgeon were released during 1994–2011 and recaptured at a rate of 1.9%, whereby estimates for the annual survival of age‐0 (Ø = 0.048) and >age‐1 (Ø = 0.931) were similar to those previously reported, but the age‐1 (Ø = 0.403) survival estimate was 52% lower. Post hoc analysis using time‐specific survival estimates indicated lower survival for age‐1 fish post‐2003 year classes, relative to the pre‐2002 year classes. An analysis confirms that hatchery‐reared pallid sturgeon continue to survive in the wild. However, low survival during the first 2 years of life is a management concern as efforts are aimed at maximizing genetic diversity and population growth. A follow‐up analysis also demonstrated the variability of capture rates and survival over time, which reinforces the need to continue to monitor and evaluate mark‐recapture data. The mark‐recapture efforts have provided demographic parameter estimates that remain a critical component for species recovery as these data are incorporated into population models.
      PubDate: 2015-11-04T03:25:20.236231-05:
      DOI: 10.1111/jai.12929
  • Champsodontids at the gates: first record of Champsodon vorax
           Günther, 1867 from the Aegean Sea (Teleostei: Champsodontidae)
    • Authors: S. Yapici; R. Fricke, H. Filiz
      PubDate: 2015-10-28T02:08:57.14124-05:0
      DOI: 10.1111/jai.12931
  • Effects of freezing on white perch Morone americana (Gmelin, 1789):
           implications for multivariate morphometrics
    • Abstract: This study tested the hypothesis that duration of freezing differentially affects whole‐body morphometrics of a derived teleost. Whole‐body morphometrics are frequently analyzed to test hypotheses of different species, or stocks within a species, of fishes. Specimens used for morphometric analyses are typically fixed or preserved prior to analysis, yet little research has been done on how fixation or preservation methods or duration of preservation of specimens might affect outcomes of multivariate statistical analyses of differences in shape. To determine whether whole‐body morphometrics changed as a result of freezing, 23 whole‐body morphometrics of age‐1 white perch (Morone americana) from western Lake Erie (n = 211) were analyzed immediately after capture, after being held on ice overnight, and after freezing for 100 or 200 days. Discriminant function analysis revealed that all four groups differed significantly from one another (P 
      PubDate: 2015-10-28T02:06:40.707759-05:
      DOI: 10.1111/jai.12930
  • Length–weight and length–length relationships for six flounder
           species (Pleuronectiformes) from the eastern coast of Korea
    • Authors: S. N. Kwak; J. M. Park
      Abstract: The relationships between total length and wet weight and between total length and standard length are presented for six flounder species from the eastern coast of Korea between 2011 and 2012: Paralichthys olivaceus (Temminck & Schlegel, 1846), Cleisthenes pinetorum (Jordan & Starks, 1904), Glyptocephalus stelleri (Schmidt, 1904), Platichthys stellatus (Pallas, 1787), Pseudopleuronectes herzensteini (Jordan & Snyder, 1901), and Pseudopleuronectes yokohamae (Günther, 1877). The values of exponent b, estimated using nonlinear least squares of weight and length data, ranged from 3.076 to 3.469. All relationships between total length and standard length were linear (all r2 > 0.948). Fisheries scientists apply these size conversions (total length calculated from standard length) to understand several aspects of the population dynamics.
      PubDate: 2015-10-26T06:16:33.45803-05:0
      DOI: 10.1111/jai.12932
  • Length–weight relationship of seven marine catfishes (Siluriformes:
           Ariidae) in the Amazon Coastal Zone (Brazil)
    • Authors: B. E. Soares; A. C. C. Santos, F. L. S. Mendes, R. B. Barthem, L. F. A. Montag
      Abstract: Length–weight relationships (LWR) were determined for seven commercially important species of catfishes (Siluriformes, Ariidae) from the Amazon Coastal Zone (ACZ), Brazil. The analyses included 3628 specimens sampled in three trawling expeditions: dry season of 1996 (111 hauls), rainy season of 1997 (120 hauls), and dry season of 1997 (120 hauls). Bottom‐trawl nets were utilized for sampling. Length‐weight estimates for Amphiarius phrygiatus, Amphiarius rugispinis, Aspistor quadriscutis, Notarius grandicassis, Sciades couma, Sciades parkeri and Sciades proops.
      PubDate: 2015-10-26T06:12:46.689112-05:
      DOI: 10.1111/jai.12927
  • Length–weight relationships for eight fish species from the Pearl
           River Delta, China
    • Abstract: Length–weight relationships (LWRs) were estimated for eight native fish species collected in the lower reaches of the Pearl River, Guangdong Province, South China. Fish were sampled in March and September 2014 and January 2015, using gillnets with various mesh sizes (5–8 cm) and trawl nets with a 3 cm mesh size. A total of 568 specimens of the eight species were analyzed. The r2 values for these species ranged from 0.951 to 0.991. The b values ranged from 3.00 for Takifugu ocellatus and Moolgarda cunnesius, to 3.57 for Clupanodon thrissa. New maximum total lengths are recorded for Takifugu ocellatus (17.6 cm) and Liza carinata (24.4 cm). For five species (Clupanodon thrissa, Megalobrama terminalis, Collichthys lucidus, Coilia grayii, Takifugu ocellatus), the LWRs are presented to fishbase for the first time.
      PubDate: 2015-10-26T06:10:05.874084-05:
      DOI: 10.1111/jai.12925
  • Confirmation of the presence of the Indian stinging catfish,
           Heteropneustes fossilis (Bloch, 1794) (Heteropneustidae) in Syrian inland
    • Authors: A. S. Ali; L. A. Jawad, A. A. Saad
      PubDate: 2015-10-26T06:09:44.204042-05:
      DOI: 10.1111/jai.12926
  • Length–weight and length–length relationships of four native
           fish species of the Qinghai‐Tibet Plateau, China
    • Authors: Y. Y. Liang; D. K. He, X. Y. Sui, Z. X. Shen, W. Xiong, Y. F. Chen
      Abstract: This study presents the length–weight (LWR) and length–length relationships (LLR) of four fish species (Gymnocypris namensis Wu & Ren, 1982; Herzensteinia microcephalus Herzenstein, 1891; Triplophysa stenura Herzenstein, 1888; and Triplophysa brevicauda Herzenstein, 1888) collected from the Qinghai‐Tibet Plateau, China. A total of 291 specimens was collected using electric fishing (12V), gill nets (mesh size: 2 × 2 cm, 3 × 3 cm) and cast nets (mesh size: 2 × 2 cm), in July 1998 and October 2014.
      PubDate: 2015-10-22T07:16:52.254834-05:
      DOI: 10.1111/jai.12909
  • Length–weight relationships of two fish species from Gamsiab
           Reservoir, western Iran: Alburnus mossulensis Heckel, 1843 and Luciobarbus
           esocinus Heckel, 1843
    • Authors: S. A. Hedayati; H. Ghafari Farsani, M. H. Gerami
      Abstract: In this study the length–weight relationships (LWRs) for two fish species, Alburnus mossulensis and Luciobarbus esocinus, were analyzed based on seasonal samplings from 2013 and 2014. The specimens were collected by cast‐ and dip net from the Karun River Basin in the Zagros Mountains, Iran. The b values in the LWRs were determined as 2.9996 for A. zagrosensis and 2.6546 for L. esocinus.
      PubDate: 2015-10-12T01:47:38.363707-05:
      DOI: 10.1111/jai.12915
  • Length–weight relationships of five fish species from the Hongshui
           River, China
    • Abstract: The length–weight relationships (LWRs) of five Chinese endemic fish species caught by cast nets (mesh size 1 cm) and gillnets (mesh size 4 cm) in the Hongshui River in June and July 2011 and October to November 2012 were determined. These are the first LWRs records for the five little‐known species.
      PubDate: 2015-10-09T06:41:38.989343-05:
      DOI: 10.1111/jai.12921
  • Length–weight relationships of five fish species collected from
           Basistha River, a torrential river in Assam, India
    • Authors: J. F. Hussain; M. K. Das, G. Kaushik, S. Bordoloi
      Abstract: Length–weight relationships (LWRs) are presented for five species, namely: Garra nasuta (McClelland, 1838); Garra gotyla gotyla (Gray, 1830); Devario aequipinnatus (McClelland, 1839); Barilius bendelisis (Hamilton, 1807) and Pseudolaguvia shawi (Hora, 1921) collected from the Basistha River, Guwahati, Assam, India. Information regarding LWR data for four of these species was not yet available in FishBase (Version 04/2015; Froese and Pauly, 2015).
      PubDate: 2015-10-09T06:36:01.519174-05:
      DOI: 10.1111/jai.12913
  • The origin and divergence of Gobioninae fishes (Teleostei: Cyprinidae)
           based on complete mitochondrial genome sequences
    • Authors: J. Zhao; D. Xu, K. Zhao, R. Diogo, J. Yang, Z. Peng
      Abstract: The gudgeons (subfamily Gobioninae) are a group of cyprinid fishes primarily distributed in East Asia. However, studies on their origins and divergence are scarce. Here the whole mitochondrial genome sequences of 27 gudgeon species (including one newly determined), 22 other cyprinid species, and two non‐cyprinids as outgroups are applied to infer the evolution of the gudgeons. Based on Bayesian and maximum likelihood phylogenetic analyses, the gudgeons were determined to be a monophyletic group which can be further subdivided into four monophyletic clades with strong supports. The divergence times of the gudgeons were estimated using a relaxed molecular clock method; the results indicate that these fishes originated in the early Paleocene (approx. 63.5 Mya) and that the basal Hemibarbus group diverged from the other gudgeon fishes (approx. 58.3 Mya). As an independent group the Coreius began to diverge from the remaining two groups (approx. 54.6 Mya); the most derived two groups diverged from each other (approx. 53.6 Mya). The divergences of the four gudgeon groups were within a relatively short time frame (approx. 58–53 Mya). Based on the reconstruction of evolutionary trends of gudgeon habitat, evidence is provided that supports the origin and differentiation of this fauna as being associated with some special paleo‐environmental events occurring from the early Paleocene to the Pliocene. The study represents comprehensive molecular dating and character evolution analyses of the gudgeons, and providing a valuable framework for future research in the evolution of the Gobioninae fishes.
      PubDate: 2015-10-05T02:05:04.106875-05:
      DOI: 10.1111/jai.12920
  • Length‐weight relationships for six freshwater fish species from the
           Seyhan Reservoir (south‐eastern Anatolia, Turkey)
    • Authors: S. A. Erguden
      Abstract: Length–weight relationships (LWRs) were evaluated for six freshwater species from the Seyhan Reservoir (south‐eastern Anatolia, Turkey): Alburnus orontis, Capoeta angorae, Capoeta erhani, Garra rufa, Luciobarbus pectoralis and Salaria fluviatilis. A total of 525 specimens were captured from five locations using gill nets, scoop nets, and trammel nets of various mesh sizes in November 2012, March 2013 and September 2013. Regressions of all species were found to be significant. These are the first LWR reports for these species from the Seyhan River basin, and the first LWR reports for three of these species (Alburnus orontis, Garra rufa, Luciobarbus pectoralis) from inland waters of Turkey.
      PubDate: 2015-10-03T02:35:37.52581-05:0
      DOI: 10.1111/jai.12905
  • Factors affecting morphological development of the sagittal otolith in
           juvenile and adult small yellow croaker (Larimichthys polyactis Bleeker,
    • Abstract: The morphology and morphometrics of the sagittal otoliths of small yellow croaker (Larimichthys polyactis) from the southern Yellow Sea were investigated. Study objectives were to evaluate the shape variability and morphometric variables of sagittae of juveniles and adults as related to developmental changes and habit shift. A total of 152 fish were sampled from April to June of 2012 and 2013, along the coastal waters of the Lüsi spawning ground in the southern Yellow Sea. Changes in otolith shapes from the juvenile to the adult were presented with the rim development through the entire‐lobed‐entire transition and with the curvature of the cauda toward the ventral margin. The otolith elongation in the juvenile stage occurred at 10–20 mm standard length (SL) and was likely associated with the formation of otolith accessory growth centers from larvae to juvenile. The L. polyactis sagittal otoliths acquired their definitive shape at 130 mm SL maturity. Ontogeny on otolith shape might be related, for example, to the factors of metamorphic development, feeding habitat, and ambient water salinity, which varied throughout the growth of L. polyactis.
      PubDate: 2015-10-01T01:06:04.46102-05:0
      DOI: 10.1111/jai.12914
  • Dietary metyrapone blocks cortisol synthesis in pacu, Piaractus
           mesopotamicus (Holmberg, 1887), stressed by air exposure
    • Authors: F. S. Zanuzzo; E. C. Urbinati
      Abstract: Tested was the efficiency of dietary metyrapone (MTP) in the inhibition of cortisol biosynthesis, as a tool to elucidate the effects of this hormone in the tropical fish pacu (Piaractus mesopotamicus H.) during air‐exposure‐induced stress. Moreover, MTP efficiency was shown to be affected by both the dose and time of administration and that a daily dose of 30 mg kg−1 body weight for 10 days reduced the levels of circulating cortisol in stressed fish. The tested method of MTP administration, including the concentration and the time of administration, efficiently regulated cortisol synthesis in a non‐invasive manner.
      PubDate: 2015-10-01T01:05:39.189979-05:
      DOI: 10.1111/jai.12916
  • Length‐weight and length‐length relationships of the genus
           Alosa (Clupeoidei: Clupeiformes: Clupeidae) along the southern Caspian Sea
    • Abstract: Length–weight (LWR) and length–length (LLR) relationships were estimated for four shad species of the genus Alosa, reported from along the southern Caspian Sea coast, north of Iran in a study from March 2014. Two of these species are endemic to the Caspian Sea. The length–weight parameter b for these species ranged from 2.99 to 3.24, with regression coefficients (r2) ranging from 0.91 to 0.99. All LLRs were highly significant (r2 > 0.96).
      PubDate: 2015-09-28T02:06:14.101016-05:
      DOI: 10.1111/jai.12908
  • Microsatellites from the genome and the transcriptome of the tetraploid
           Adriatic sturgeon, Acipenser naccarii (Bonaparte, 1836) and
           cross‐species applicability to the diploid beluga sturgeon, Huso
           huso (Linnaeus, 1758)
    • Authors: E. Boscari; M. Vidotto, D. Martini, C. Papetti, R. Ogden, L. Congiu
      First page: 977
      Abstract: Ninety‐five microsatellite loci were isolated from the output of two Next Generation Sequencing projects: a genomic Illumina RADSeq and a transcriptomic Roche 454 cDNA sequencing of the Adriatic sturgeon (Acipenser naccarii). From a total of 7697 loci identified, 72 were characterized and 57 were polymorphic in the tetraploid A. naccarii, providing new markers suitable for parental allocations to be performed for future conservation actions. Loci isolated from the transcriptome showed a higher level of polymorphism, which could be related to the different sequencing approaches used. Notably, a higher rate of duplication was observed in the transcribed loci, pointing to a preferential retention of sequences with a functional role after whole genome duplications occurred in sturgeons. Cross‐species amplification of these markers was also assessed in the diploid beluga sturgeon (Huso huso), with the aim of setting up a panel of markers to be applied in future conservation programmes regarding this species.
      PubDate: 2015-09-22T07:06:32.793798-05:
      DOI: 10.1111/jai.12906
  • Laboratory test of suspended sediment effects on short‐term survival
           and swimming performance of juvenile Atlantic sturgeon (Acipenser
           oxyrinchus oxyrinchus, Mitchill, 1815)
    • Authors: J. L. Wilkens; A. W. Katzenmeyer, N. M. Hahn, J. J. Hoover, B. C. Suedel
      First page: 984
      Abstract: Tested was the hypothesis that juvenile Atlantic sturgeon (Acipenser oxyrinchus oxyrinchus) would exhibit no response in short‐term survival or swimming performance when exposed to varying concentrations of suspended sediment simulating dredge plumes in waterways where this species may be impacted by dredging operations. Sediment collected from Savannah Harbor, South Carolina, USA was used to simulate a worst‐case scenario. Juvenile sturgeon were contained for a 3‐day period in flow‐through aquaria, with limited opportunity for movement, in sediment of varying concentrations (100, 250 and 500 mg L−1 total suspended solids [TSS]) mimicking prolonged exposure to suspended sediment plumes near an operating dredge. Of the 90 fish exposed, 86 (96%) survived the test. Of the four fish that died, one was exposed to 250 TSS and three to 500 TSS. Swimming performance results indicated that nearly all fish were positively rheotactic. Critical swim speeds (Ucrits) were moderate, whether measured as absolute values (21–31 cm s−1) or as relative values (1.4–2.1 body lengths s−1), with no significant differences among treatments (F 
      PubDate: 2015-09-02T05:17:14.022844-05:
      DOI: 10.1111/jai.12875
  • Short‐term survival and dispersal of hatchery‐reared juvenile
           pallid sturgeon stocked in the channelized Missouri River
    • Authors: B. L. Eder; K. D. Steffensen, J. D. Haas, J. D. Adams
      First page: 991
      Abstract: In the summer and fall of 2014, Nebraska Game and Parks Commission crews monitored juvenile pallid sturgeon, Scaphirhynchus albus (Forbes & Richardson, 1905) (age‐1 and age‐4) implanted with telemetry tags that were stocked in a side channel of the Missouri River in Nebraska, USA to gain knowledge into post‐stocking survival and dispersal. For this study, specific questions were asked: (i) what is the short‐term survival of stocked pallid sturgeon, (ii) do pallid sturgeon stocked in summer exhibit decreased survival rates due to increased water temperatures or high river discharge, and (iii) how quickly do pallid sturgeon disperse from a stocking site' Detection histories for two rounds were used to estimate apparent survival (Ø) and detection (p) rates using maximum likelihood estimators based on the standard mark‐recapture Cormack‐Jolly‐Seber model structure within program MARK. Overall apparent survival was 98.6% and dispersal was rapid; therefore, moratoriums on stocking pallid sturgeon during summer months or during high flow events do not appear to be warranted.
      PubDate: 2015-09-01T00:22:40.253327-05:
      DOI: 10.1111/jai.12881
  • Anesthesia of wild female Persian sturgeon, Acipenser persicus (Borodin,
           1897) breeders during controlled propagation: effects on hematological
           parameters, stress response and reproductive performance
    • Authors: M. Mazandarani; S. M. Hoseini, R. Shahriari
      First page: 997
      Abstract: This study examined the effects of anesthesia on the hematological and biochemical parameters as well as the reproductive performance of wild female Persian sturgeon, Acipenser persicus, during controlled spawning. Fourteen mature females were divided into two groups: ‘anesthetized’ and ‘non‐anesthetized’. All activities including transportation, catheterizing and handling were performed with both groups: (i) under anesthesia (150 ppm clove oil), and (ii) without anesthesia. After 10 days storage and handling, blood samples were taken from all fish after anesthesia. No significant differences were found in the reproductive performance of either group. However, differences were found in the hematological parameters, with values being significantly higher in the non‐anesthetized group, including neutrophils (34.36 ± 6.33% vs 23.63 ± 5.22%), monocytes (2.84 ± 1.70% vs 1.27 ± 0.64%), mean corpuscular volume (321.3 ± 39.40 pg vs 228.0 ± 24.46 pg) and mean corpuscular hemoglobin (106.9 ± 15.70 fl vs 76.50 ± 7.50 fl). Significantly lower values were found in the non‐anesthetized group for lymphocytes (60.68 ± 7.25% vs 73.54 ± 4.80%), Hb (4.62 ± 0.74 mg dl−1 vs 6.28 ± 1.21 mg dl−1), Hct (13.86 ± 1.76% vs 18.84 ± 3.85%), red blood cell (0.43 ± 0.05 cell mm−3 vs 0.85 ± 0.13 × 106 cell mm−3) and white blood cell (22 403 ± 2240 cell mm−3 vs 35 318 ± 3084 cell mm−3). The non‐anesthetized fish had significantly higher cortisol levels compared to the anesthetized group (62.33 ± 8.85 ng ml−1 vs 46.12 ± 8.07 ng ml−1). There was no difference in plasma glucose levels between groups. It is concluded that the use of clove oil as an anesthetic is suitable for handling of wild female Persian sturgeon in controlled propagation programmes. However, further research is needed to determine a standardized protocol for the safe application of anesthesia for use in sturgeons in general.
      PubDate: 2015-08-26T07:38:11.253698-05:
      DOI: 10.1111/jai.12866
  • Confirmation of induced hybrid from female ship sturgeon (Acipenser
           nudiventris Lovetsky, 1828) and male Siberian sturgeon (Acipenser baerii,
           Brandt, 1869) using microsatellite markers
    • Authors: M. Hassanzadeh Saber; S. Baradaran Noveiri, M. Pourkazemi, M. R. Nowruzfashkhami, M. Yarmohammadi, M. A. Salari Aliabadi, H. Zolgharnein, M. T. Ronagh
      First page: 1002
      PubDate: 2015-10-03T02:36:20.220106-05:
      DOI: 10.1111/jai.12878
  • Biochemical and genetic characterization of European silver eels, Anguilla
           anguilla (Linnaeus, 1758), from the Comacchio lagoon (Italy)
    • First page: 1006
      Abstract: The European eel Anguilla anguilla has declined significantly over recent decades, exceeding its safe biological limits and probably reaching a historical minimum (1% of the 1960 recruitment level). Twenty‐three migrating females at the IV stage of silvering were collected from the Comacchio lagoon (Northern Italy); they showed high levels of cholesterol and triglycerides, probably due to lipid accumulation before migration across the Atlantic Ocean. Electrophoretic patterns showed high amounts of putative α and β globulins, probably due to high concentrations of lipoproteins. Genetic results obtained from a total of 49 specimens (20 from the Comacchio lagoon and 29 from Val Dogà) agree with the panmixia hypothesis, lacking any genetic differentiation over time and space at neutral loci. In conclusion, despite the small sampling and the population decline in recent years, high levels of variability were recorded.
      PubDate: 2015-09-22T06:52:06.144196-05:
      DOI: 10.1111/jai.12889
  • Using otolith shape and morphometry to identify four Alburnus species
           (A. chalcoides, A. escherichii, A. mossulensis and
           A. tarichi) in Turkish inland waters
    • First page: 1013
      Abstract: Asteriscus otolith shapes as well as their morphometry and shape contours were investigated in order to identify four allopatric Alburnus species: A. chalcoides (Güldenstädt, 1772) (Ordu), A. escherichii Steindachner, 1897 (Eskişehir), A. mossulensis Heckel, 1843 (Tunceli), and A. tarichi (Güldenstädt, 1814) (Van) in Turkish inland waters. These were compared using the shape indices (form factor, roundness, circularity, ellipticity, rectangularity and aspect ratio), and the morphological characters [otolith weight (OWE), otolith length (OL), otolith width (OW), otolith perimeter (OP), and otolith area (OA)]. The overall canonical discriminant analysis (CDA) classification score was 93.8%, with the lowest score for A. escherichii (82.5%) and the highest for A. chalcoides (100%). The otolith shapes, morphology and shape contours of all sampled fish were a clear species differentiator, thereby demonstrating that the otolith shape is species‐specific. The current study presents for the first time comprehensive variation information on interspecific left‐right asteriscus otoliths in males and females of each Alburnus species: A. chalcoides from Ordu, A. escherichii from Eskişehir, A. mossulensis from Tunceli and A. tarichi from Van, based on a total of 307 individuals. Scanning electron microscopy (SEM) images, shape contours and other otolith characters vary within the same genus; these differences should be investigated not only in other freshwater fish species or genera but also in the same species living in different habitats. In addition, further investigation is required not only with respect to the morphometry, biometry, shape, geometry, and shape contours of the otoliths, but also regarding the genetic methods for robust identification of various sympatric and allopatric fish populations.
      PubDate: 2015-08-25T04:42:01.546444-05:
      DOI: 10.1111/jai.12860
  • Otolith microstructure reveals consequences for juvenile growth of
           fractional spawning in an invasive goldfish Carassius auratus (Linnaeus,
           1758) population
    • First page: 1029
      Abstract: The consequences of fractional spawning on the early‐life growth rates of invasive goldfish (Carassius auratus) from the Qinghai‐Tibet Plateau were studied using the otolith microstructure of samples collected in June 2011. The effect of the estimated hatching date on the subsequent growth of individual fish was determined by back‐calculating their number of growth days, daily growth rates and the onset of their second growth season. The number of growth days in the first growth season ranged from 93 to 186 days. Following hatching, daily growth rates increased rapidly to a maximum of 0.55 mm days−1 before declining to 0.09 mm days−1. The effect of the duration of the first growth season on individuals was significant (P 
      PubDate: 2015-10-05T01:31:25.374327-05:
      DOI: 10.1111/jai.12900
  • Structure of fish assemblages in seven tropical reservoirs in southeastern
           Brazil during the rainy season; what matters: physico‐chemical or
           hydrological connectivity influences'
    • First page: 1034
      Abstract: Closely connected reservoirs were hypothesized to have more similar fish compositions than do spatially isolated reservoirs, and that environmental variables play an important role in shaping assemblage structure on a local scale. Fish assemblages and environmental variables were sampled in seven reservoirs constructed to generate hydroelectricity, with most inflowing water coming directly or via transposition of the Paraiba do Sul River, in Southeastern Brazil. Using gill nets and environmental measurement variables, fish collections were carried out between January and February 2008 (wet season), when increases in temperature and rainfall increase hydrological connectivity, facilitating fish movements throughout the river‐reservoir system. Mean fish density, biomass and the number of species did not differ significantly among the seven reservoirs. On the other hand, the assemblage structure differed among almost all pairs of reservoirs. Of the reservoirs connected in series, only two (Santana and Vigário) showed a similar assemblage structure, suggesting that hydrological connectivity plays a minor role. Physico‐chemical variables changed among reservoirs and seemed to play an important role in determining the assemblage structure, with higher conductivity (average = 108 μS cm−1) and TDS – total dissolved solid (0.07 g L−1), and low pH (5.8) in the Pereira Passos Reservoir associated with a high number of the Siluriformes Loricariichthys castaneus, and with the opposite conditions in Tocos (conductivity = 27 μS cm−1; TDS = 0.02 g L−1; pH = 7) that were associated with a high number of the carnivorous Characiformes Oligosarcus hepsetus and Hoplias malabaricus. A direct relationship was found between abundance of the catfishes Pimelodus fur and Glanidium albescens and high turbidity (90.4 NTU) and low temperature (25.3°C) and transparency (0.1 m) in the Ilha dos Pombos Reservoir. Moreover, a close relationship was found between high abundance of the cichlid Cichla kelberi and the high temperature and transparency of Lajes (28.3°C; 2.2 m) and Santa Branca (27.1°C; 2.6 m) reservoirs. These findings support the hypothesis that physico‐chemical variables structure fish assemblages in reservoirs, but that hydrological connectivity seems to play a minor role in shaping assemblage structure. However, other unmeasured variables (e.g. physical habitat characteristics and biotic interactions) and other seasons need to be evaluated in further studies.
      PubDate: 2015-08-25T05:26:59.289415-05:
      DOI: 10.1111/jai.12864
  • Parasites component community in wild population of Pterophyllum scalare
           Schultze, 1823 and Mesonauta acora Castelnau, 1855, cichlids from the
           Brazilian Amazon
    • First page: 1043
      Abstract: The aim of the present study was to compare the component parasite communities of the Pterophyllum scalare and Mesonauta acora cichlids in the Amazon River system in northern Brazil. From September to December 2012, 42 specimens of P. scalare and 38 specimens of M. acora were captured using hand nets and gillnets in the Igarapé Fortaleza basin, a tributary of the Amazon River in the state of Amapá. Of the P. scalare specimens examined, 97.6% were parasitized by Ichthyophthirius multifiliis, Tripartiella sp., Trichodina nobilis, Gussevia spiralocirra, Posthodiplostomum sp., Capillaria pterophylli, Ichthyouris sp. and Gorytocephalus spectabilis. Similarly, all specimens of M. acora were parasitized by I. multifiliis, Tripartiella sp, T. nobilis, Sciadicleithrum joanae, Posthodiplostomum sp., Pseudoproleptus sp., Ichthyouris sp. and G. spectabilis. However, for both hosts the dominance was of I. multifiliis and with an overdispersion of parasites. Parasite communities of P. scalare and M. acora were similar and only Pseudoproleptus sp. and Posthodiplostomum sp. were larvae. Brillouin diversity, parasite species richness and evenness were higher for M. acora than for P. scalare, which presented a negative correlation of parasite abundance with body size. Both cichlid species had parasite communities characterized by low diversity and low species richness, with a predominance of ectoparasite species and greatest richness of helminth species, with a low abundance of endoparasites. This was the first study on the parasite diversity in wild P. scalare and M. acora.
      PubDate: 2015-09-22T07:04:14.826271-05:
      DOI: 10.1111/jai.12903
  • Seasonality affects the parasitism levels in two fish species in the
           eastern Amazon region
    • First page: 1049
      Abstract: Fish parasite communities have strong interactions with the environment, the host fish, and the aquatic invertebrate communities. Thus, factors directly involving their different life cycles, such as different host populations and environments, as well as seasonal fluctuations in water levels can cause different responses. The main factors structuring parasite communities may be the hydrodynamic variations, together with seasonal variations in the availability of infectious stages of parasites in the environment. The purpose of this study was therefore to investigate the influence of the rainy and dry seasons on parasite infracommunities in fish species in the Amazon River System of Brazil. Between October 2009 and April 2011, specimens of Colossoma macropomum and Colossoma macropomum × Piaractus brachypomus hybrids (tambatinga) were examined for the presence of parasites. The parasite communities were similar in these two species that both showed aggregate dispersion, although the hybrid specimens were less parasitized. For both hosts, the Brillouin diversity, species richness, evenness and Berger‐Parker dominance indices were similar in the rainy season and dry season, except that the Berger‐Parker dominance index for the C. macropomum × P. brachypomus hybrid was higher during the dry season. Ichthyophthirius multifiliis was the dominant parasite, followed by Piscinoodinium pillulare, in both hosts. However, C. macropomum had a higher prevalence and abundance in the dry season, while in the C. macropomum × P. brachypomus hybrid, only the I. multifiliis prevalence increased. The abundance of monogenean species (Anacanthorus spathulatus, Linguadactyloides brinkmanni, Mymarothecium and Notozothecium janauachensis) was higher during the dry season in the gills of C. macropomum. Despite overdispersion of the lernaeid Perulernaea gamitanae, a seasonal pattern was seen only in the C. macropomum × P. brachypomus hybrid, with higher prevalence in the rainy season. The low prevalence and abundance of the ectoparasites Tetrahymena sp., Trichodina sp. and Braga patagonica, as well as of the endoparasites Procamallalus (Spirocamallanus) inopinatus and Neoechinorhynchus buttnerae, were not affected by seasonality. This information is applicable in farming practice for these economically important fish, as it indicates the best time for prophylactic management and treatment against parasites, in order to prevent economic losses in fish farms.
      PubDate: 2015-08-26T07:40:07.531064-05:
      DOI: 10.1111/jai.12865
  • Age, growth, and reproduction of blackspot snapper Lutjanus fulviflammus
           (Forsskål 1775) around Yaeyama Islands, southern Japan, between 2010
           and 2014
    • Authors: T. Shimose; A. Nanami
      First page: 1056
      Abstract: Age, growth, and reproductive characteristics of blackspot snapper Lutjanus fulviflammus around the Yaeyama Islands (24°N, 124°E) in southern Japan were investigated between 2010 and 2014. Samples were caught by angling or purchased monthly at fish markets to cover all size ranges and seasons. Specimens consisted of 280 females (176–347 mm fork length), 177 males (193–325 mm), and 17 juveniles (42–128 mm). Spawning was confirmed from April to August by the presence of postovulatory follicles, which, based on the occurrence of yolked oocytes possibly also occurred in March and September. Maximum fork length and age in the specimens around Yaeyama Islands were 347 mm and 23 years for females and 325 mm and 21 years for males. These results were compared to those reported previously for the species in other regions by standardized criteria. Possible spawning duration of 7 months in the population around Yaeyama Islands is similar to that in New Caledonia (22°S, 5 months) and Okinawa Island (26°N, 6 months), but shorter than that in Tanzania (8°S) and the southern Arabian Gulf (25°N) (~12 months). The maximum body sizes and ages around Yaeyama Islands are also similar to the populations in New Caledonia and Okinawa Island (>300 mm FL, >20 years old), but larger/older than those in Tanzania and the southern Arabian Gulf (
      PubDate: 2015-09-07T23:55:42.811027-05:
      DOI: 10.1111/jai.12894
  • Recruitment in Japanese sea bass, Lateolabrax japonicas (Cuvier, 1828):
           effects of timing on spawning and larval quality and quantity
    • Authors: M. S. Islam; M. Ueno, Y. Yamashita
      First page: 1064
      Abstract: This study aimed to elucidate the causes of variability in larval survival and juvenile abundance (recruitment) within and among cohorts of Japanese sea bass (JSB; Lateolabrax japonicus), a winter‐spawning temperate coastal marine fish. Larvae and settled individuals (settlers) belonging to four cohorts were collected from Tango Bay (the Sea of Japan coast) during eight sampling cruises in 2007 and 2008. Larvae were sampled in January and February each year using an ichthyoplankton net, and settlers were collected in February and March each year using a beam trawl. Age of individual larva and settlers was determined and growth history was back‐calculated from otolith microstructure, and the hatch date distribution was computed. Temperature, daily growth rate, size‐at‐age, hatch date, and density data of larvae and settlers allowed elucidating the effects of the timing of spawning and larval quantity and quality (growth rate and body size) on larval survival and recruitment within and among cohorts of JSB. Results showed that cohorts that hatched earlier in the season had higher quantity of larvae, experienced higher mean temperatures and survived better than cohorts hatched later. Recruitment variability among cohorts is determined largely by the initial quantity of larvae, as this explained >97% of the variability in recruitment among cohorts. Within cohorts, larger hatched larvae grew faster than their smaller conspecifics, and the bigger and faster growing larvae survived and settled. Results from this study suggest the following scenarios for recruitment of JSB: (i) earlier spawning in the season promotes larval survival since earlier cohorts are likely to encounter a better temperature and perhaps food conditions, and therefore recruit better than later cohorts; (ii) the initial quantity of larvae appears to be an important determinant of recruitment variability among cohorts; and (iii) the size‐ and growth‐related mechanisms operating during the larval phase appear to start at the time of the hatch.
      PubDate: 2015-09-01T00:18:51.246351-05:
      DOI: 10.1111/jai.12861
  • New inland sites for the Mediterranean killifish (Aphanius fasciatus
           Valenciennes, 1821) in the Sahara Desert of Algeria
    • Authors: R. Chaibi; A. Si Bachir, H. Chenchouni
      First page: 1072
      Abstract: The current study aims to update the range of Aphanius fasciatus occurring in inland aquatic systems, and to characterise the length–weight relationship of specimens caught in inland waters of the Northern Sahara of Algeria. Although the known distribution of A. fasciatus is restricted to coastal waters including islands of the Mediterranean region, visits to 39 hydrosystems in Northeast Algeria revealed the presence of the species at two sites: Oued Still and Lake Temacine. Both sites are in the Sahara Desert of Algeria, where the climate is hot and hyperarid. The surveyed populations perform their entire life cycle at the same sites. Analysis of a sample composed of 27 individuals captured in Oued Still allowed determination of the length–weight relationship. On average, females measured 4.2 ± 0.6 cm in length and weighed 1.1 ± 0.5 g; males were 3.2 ± 0.6 cm and 0.5 ± 0.2 g. Growth parameters of the von Bertalanffy equation for A. fasciatus were estimated at Lt = 5.5145 (1−exp (−0.341(t−0.0049))), Wt = 2.6692 (1−exp (−0.271t))3.
      PubDate: 2015-09-22T06:57:33.133105-05:
      DOI: 10.1111/jai.12892
  • Dietary inclusion of mussel meal enhances performance and improves feed
           and protein utilization in common sole (Solea solea, Linnaeus, 1758)
    • Authors: F. Mongile; L. Mandrioli, M. Mazzoni, M. Pirini, A. Zaccaroni, R. Sirri, L. Parma, P. P. Gatta, G. Sarli, A. Bonaldo
      First page: 1077
      Abstract: The present study was carried out to test different mussel meal (MM) dietary levels in combination with fishmeal (FM) on the growth performance, fatty acid composition and liver histology of common sole, Solea solea juveniles to highlight the growth potential of this species. Four isoproteic (53%) and isolipidic (11%) pelletized diets were formulated to contain graded levels of mussel meal, MM0 (0%), MM25 (25%), MM50 (50%) and MM75 (75%), up to 75%. Sole juveniles (initial individual mean body weight 13.1 ± 2.3 g, n = 840) were fed to satiation for 91 days. Seventy fish per tank (500‐L, 0.64 m2 bottom surface) were reared in 12 tanks (3 tanks per treatment) at 20 ± 1°C. Diets containing MM (MM25, MM50 and MM75) gave a significantly higher specific growth rate (SGR, 1.27 ± 0.01, 1.38 ± 0.06 and 1.40 ± 0.05, respectively), higher feed intake and lower feed conversion rate (FCR, 1.09 ± 0.01, 1.00 ± 0.04 and 0.98 ± 0.02, respectively) when compared to the FM‐based diet (MM0, SGR, 0.98 ± 0.11, FCR, 1.52 ± 0.13). Carcass proximate composition was not influenced by dietary treatments, with the exception of the significantly lower lipid content in the MM75 group. Protein efficiency ratio (PER) and gross protein efficiency (GPE) were significantly improved by the mussel meal inclusion (PER, 1.29 ± 0.12, 1.76 ± 0.01, 1.89 ± 0.06, 1.95 ± 0.08; GPE, 25.29 ± 1.85, 33.38 ± 0.89, 35.96 ± 1.36, 36.59 ± 1.05 in MM0, MM25, MM50 and MM75, respectively). A significant decrease in the viscerosomatic index was observed in fish fed with MM50 and MM75 in comparison to MM0. The hepatosomatic index of fish fed with MM0 and MM25 was higher than that of fish fed with MM75, although the histological examination of liver parenchyma in all experimental groups showed a uniformly abundant accumulation of lipid droplets. Carcass fatty acid composition was significantly affected by dietary treatments, reflecting the dietary fatty acid profile. According to these results, the inclusion of MM in experimental FM‐based diets improved the performance and feed utilization of common sole juveniles. The inclusion of MM in the present trial allowed a higher SGR than that registered in previous growth trials on common sole. This study could provide useful information to detect effective ingredients for practical diets in Solea solea. It also seems advisable to consider an inclusion of at least 25% MM in the experimental reference diet to be used for further application towards the development of specific diets for this species.
      PubDate: 2015-09-28T02:10:57.952867-05:
      DOI: 10.1111/jai.12895
  • Dietary valine requirement of juvenile blunt snout bream (Megalobrama
           amblycephala Yih, 1955)
    • First page: 1086
      Abstract: An 8‐week feeding trial was carried out to test the hypothesis that adequate dietary valine might improve growth, feed utilization and protein content in blunt snout bream, whereas a valine deficiency might have adverse effects on these parameters. Six isonitrogenous (34% crude protein) and isoenergetic (14.2 MJ kg−1 digestible energy) experimental diets were formulated to contain graded valine levels (0.66, 0.95, 1.26, 1.55, 1.87 and 2.16% of dry weight) at about 0.30% increments replaced by equal proportions of glycine. At the end of the experiment the survival rate was not significantly affected by the dietary valine level. Final weight, weight gain (WG), specific growth rate (SGR), feed efficiency ratio (FER) and protein efficiency ratio (PER) increased with an increasing dietary valine level up to the 1.26% diet, and thereafter remained relatively constant. Dietary valine levels significantly affected the viscerosomatic index, but not the hepatosomatic index or condition factor. The dietary valine levels significantly affected the protein contents of whole body and plasma. Dietary valine supplementation significantly increased the plasma valine concentration, but not the levels of other branched‐chain amino acids (isoleucine and leucine). Based on SGR and FER, the optimal dietary valine requirements of juvenile blunt snout bream were determined to be 1.32% of the diet (3.88% of dietary protein) and 1.26% of the diet (3.71% of dietary protein), respectively, using broken‐line regression analysis.
      PubDate: 2015-10-05T01:30:10.976368-05:
      DOI: 10.1111/jai.12911
  • Optimization of the culture methods for nursing Chinese black sleeper
           (Bostrychus sinensis Lacépède, 1801) fry using an
           orthogonal array design
    • Authors: Y. T. Zhang; Z. Li, S. X. Chen, Y. Mao, W. S. Hong
      First page: 1096
      Abstract: Chinese black sleeper (Bostrychus sinensis) is a burrow‐dwelling fish found in intertidal mudflats. As a commercially important fish in southern China, the nursing of B. sinensis fry is a critical step for its farming. The growth and survival of B. sinensis fry are closely related to the stocking density, shelter and diet. In this study, 1575 healthy fry were used to investigate the effects of three factors, namely: diet, stocking density, and shelter on the survival rate (SR), specific growth rate (SGR) and condition factor (CF). The optimal combinations of three levels of each of these three factors (A1‐3, B1‐3 and C1‐3) were also determined, using an orthogonal array design of OA9 (34). After 50 days of nursing, the results showed that: (i) the best combinations for the three factors at their optimal levels were A1B1C2 or A1B1C3; (ii) factor A was the most important factor and had significant effects (P  0.05); (iii) the SGR, SR and CF of fry fed with commercial formulated feed (CFF) were significantly higher (P 
      PubDate: 2015-09-07T23:51:34.008281-05:
      DOI: 10.1111/jai.12893
  • Characterization and SNP variation of the interleukin‐1β gene
           of bighead carp (Cyprinus pellegrini Tchang, 1933) and five strains of
           common carp [Cyprinus carpio Linnaeus (1758)] in China
    • First page: 1102
      Abstract: Single nucleotide polymorphisms of Interleukin‐1β (IL‐1β) have been reported as markers for susceptibility to infectious diseases in humans and livestock. The present study was to determine the genetic variation of this cytokine in six carp strains. Among the sampled individuals, a total of 13 SNPs, including eight in introns and five in coding regions, were identified at intron 5, exon 6, intron 6 and exon 7. Three positions of 1700, 1733 and 1934 resulted in variable amino acid changes with Phe to Tyr, Pro to Leu and Lys to Asn, respectively. Five positions with minor allele frequency (MAF) were larger than 0.05. Among 13 SNPs, eight positions of allele frequency and ten positions of genotypic frequency showed significant differences between some populations. The genotype distributions of the 13 SNPs were consistent with the assumption of the Hardy‐Weinberg equilibrium, with the exception of two positions in the Yibu and bighead carp (P 
      PubDate: 2015-08-27T05:51:44.848585-05:
      DOI: 10.1111/jai.12862
  • The effects of gradual or abrupt changes in salinity on digestive enzymes
           activity of Caspian kutum, Rutilus kutum (Kamensky, 1901) larvae
    • Authors: N. Gheisvandi; A. Hajimoradloo, R. Ghorbani, S. H. Hoseinifar
      First page: 1107
      Abstract: This study investigates the effects of gradual or abrupt changes in rearing salinity on food transit time and digestive enzymes activity of Caspian kutum (Rutilus kutum) larvae. The larvae (532 ± 0.05 mg) were supplied and randomly allocated into 12 tanks at a density of 45 fish per tank. Experimental treatments were fresh water (salinity 0) [FW] as control, exposure to salinity 5 [T1], and gradual transfer to salinity 10 in two steps of first to 5 h, then and after 12 h to a salinity of 10 [T2], and abrupt change (direct transfer to a salinity of 10 [T3]). Results showed at 8 h after start of feeding that the larvae intestine was filled with food pellets except in treatment T1. Enzyme activity responded to salinity change as follows: the highest trypsin, amylase, and chymotrypsin activities were observed in T1; however, these were not significantly different to treatment T3 (P > 0.05). Trypsin activity peaks in the FW and T2 groups occurred 8 h after feeding, and in T3 and T1 groups 5 h after feeding. Peak chymotrypsin and alkaline phosphatase activity was observed 5 and 8 h after feeding in all experiments, respectively. The highest α amylase activity in FW and T2 groups occurred 5 h after feeding, while in T3 and T1 these peaks were observed 8 h after feeding. These results indicate that salinity had some noticeable effects on the activities of digestive enzymes after feeding.
      PubDate: 2015-09-22T06:53:25.747568-05:
      DOI: 10.1111/jai.12891
  • Ultrastructure of grey mullet (Mugil cephalus, Linnaeus, 1758) spermatozoa
           as revealed from light, scanning and transmission electron microscopy
    • Authors: P. Kumar; V. Saranya, M. Natarajan, M. Kailasam, G. Biswas
      First page: 1113
      Abstract: Spermatozoa morphology and fine structure were studied in the grey mullet, Mugil cephalus using light, scanning and transmission electron microscopy. Light microscopy observations indicate a semi‐cystic type of spermatogenesis in the testis. The electron microscopy micrograph showed that the spermatozoon of M. cephalus is uniflagellated (total length 5.78 ± 1.26 μm), differentiated into an ovoid‐shaped head without acrosome (1.80 ± 0.35 μm in length and 1.91 ± 0.30 μm in diameter), with a short midpiece and a long cylindrical flagellar tail (length 3.60 ± 0.50 μm). The midpiece is characterized by the presence of four to five vacuoles, a cytoplasmic canal, two centriole and two spherical mitochondria having a flat type of cristae. Chromatin granules of the nucleus form an electron‐dense homogeneous mass. The flagellum consists of nine peripheral microtubules and a central pair (9 + 2) surrounded by the plasma membrane with side fins. The results confirm that spermatozoa of M. cephalus are perciform or teleostean type II. Information generated from the present study will be useful in taxonomic classification, cryopreservation and breeding work.
      PubDate: 2015-10-03T02:37:10.445536-05:
      DOI: 10.1111/jai.12902
  • Effects of sediment burial on grass carp, Ctenopharyngodon idella
           (Valenciennes, 1844), eggs
    • First page: 1120
      Abstract: It is thought that grass carp (Ctenopharyngodon idella) eggs must remain suspended in the water column in order to hatch successfully. Using sand, the effects of varying sediment levels on grass carp eggs were tested at different developmental states and temperatures. Survival was high (15–35%, depending on temperature and trial) in the unburied treatment where eggs rested on a sand bed but were not covered by sediment. Survival was lower in the partial burial (5–10%) and very low (0–4%) in the full burial treatment. In all treatments, delayed hatching (organisms remaining in membranes past the stage of hatching competence) was noted. Deformities such as missing heads and pericardial edema occurred at high rates in the partial and full burials. Eggs that come in contact with the benthos and are resuspended in the water column should be considered in embryonic drift models.
      PubDate: 2015-10-12T02:17:23.511843-05:
      DOI: 10.1111/jai.12918
  • Evaluation of three capture techniques for invasive Micropterus salmoides
           (Lacépède, 1802) in a Neotropical reservoir: implications for
           population control and management
    • Authors: V. M. Ribeiro; R. R. Braga, V. Abilhoa, J. R. S. Vitule
      First page: 1127
      PubDate: 2015-09-22T06:51:11.890902-05:
      DOI: 10.1111/jai.12904
  • Fatty acid composition of eggs and semen of Ctenopharyngodon idella
           (Valenciennes, 1844) taken for culture trials from the Keban Dam Lake,
           Elazığ, Turkey
    • First page: 1130
      PubDate: 2015-09-12T04:35:52.281092-05:
      DOI: 10.1111/jai.12880
  • Telestes miloradi Bogutskaya, Zupancic, Bogut & Naseka, 2012 and
           Delminichthys ghetaldii (Steindachner, 1882) re‐discovered in
           Croatia, requiring urgent protection
    • First page: 1133
      PubDate: 2015-09-04T02:27:24.367285-05:
      DOI: 10.1111/jai.12879
  • Histopathology of gill myxosporean infection in cultured Indian major and
           minor carps, West Bengal, India
    • Authors: S. Banerjee; G. Dash, T. J. Abraham
      First page: 1137
      PubDate: 2015-10-12T02:04:48.720966-05:
      DOI: 10.1111/jai.12917
  • Length–weight relationships for two marine fish species from the
           Gulf of Oman: Uranoscopus guttatus Cuvier, 1829 and Lagocephalus inermis
           (Temminck & Schlegel, 1850)
    • First page: 1142
      Abstract: This study presents the first report of length–weight relationships (LWRs) for Uranoscopus guttatus and Lagocephalus inermis from the Iranian coast on the Gulf of Oman. A wide range of body lengths and weights were obtained using samples from non‐selective trawling surveys. The LWRs obtained were W = 41.408L3.519 and W = 16.525L2.847 for U. guttatus and L. inermis, respectively.
      PubDate: 2015-06-24T05:36:59.034986-05:
      DOI: 10.1111/jai.12821
  • Length–weight relationship of two fish species from Poonch River,
           Western Indian Himalaya: Glyptothorax kashmirensis (Hora, 1923) and
           Crossocheilus diplochilus (Heckel, 1838)
    • Authors: N. K. Sharma; R. Singh, N. N. Pandey, M. S. Akhtar, J. I. Mir
      First page: 1144
      Abstract: This study provides the first report of length–weight relationships (LWRs) for two fish species, Glyptothorax kashmirensis and Crossocheilus diplochilus, collected from the Poonch River, one of the lesser tributaries to the Indus basin in India. New maximum length records are reported for both species.
      PubDate: 2015-06-23T02:33:43.209265-05:
      DOI: 10.1111/jai.12834
  • Length–weight relationships for eight fish species from the Ravi
           River, north‐western India
    • Authors: N. K. Sharma; J. I. Mir, R. Singh, M. S. Akhtar, N. N. Pandey
      First page: 1146
      Abstract: Length–weight relationships (LWRs) were evaluated for Badis badis (n = 25), Sperata seenghala (n = 26), Labeo gonius (n = 34), Rasbora rasbora (n = 30), Bagarius bagarius (n = 24), Gagata cenia (n = 27), Glyptothorax stoliczkae (n = 24) and Channa orientalis (n = 28) from the Ravi River tributary in North India. Altogether 218 samples of eight species were obtained between May and November 2014 using cast nets and gill nets. LWRs for these species were unknown to FishBase, and new maximum lengths were recorded for two of these species.
      PubDate: 2015-06-23T01:58:12.510793-05:
      DOI: 10.1111/jai.12836
  • Length–weight relationships for six fish species in Iskenderun Bay
           (Eastern Mediterranean Sea coast of Turkey)
    • Authors: D. Erguden; S. A. Erguden, M. Gurlek
      First page: 1148
      Abstract: The length–weight relationships (LWRs) were estimated for six non‐indigenous fish species, namely, Apogon smithi (Kotthaus, 1970); Ostorhinchus fasciatus (White, 1790); Pomadasys stridens (Forsskål, 1775); Champsodon capensis Regan, 1908; Torquigener flavimaculosus Hardy & Randall, 1983; and Tylerius spinosissimus (Regan, 1908) from Iskenderun Bay, eastern Mediterranean Sea. Their length–weight relationship b values ranged from 2.902 to 3.501, and all regressions were found to be significant for all six species (P 
      PubDate: 2015-06-30T06:39:45.046675-05:
      DOI: 10.1111/jai.12839
  • Length–weight and length–length relationships of four species
           of genus Pethia and genus Puntius from wetlands of Lakhimpur district,
           Assam, India
    • Authors: G. Kaushik; S. Bordoloi
      First page: 1150
      Abstract: Length–weight relationships (LWR) and length–length relationships (LLR) were estimated for four species, namely, Puntius sophore (Hamilton, 1822), Puntius chola (Hamilton, 1822), Pethia ornatus (Vishwanath & Laisram, 2004) and Pethia ticto (Hamilton, 1822) collected from different wetlands of Lakhimpur, Assam. The values of the parameter slope (b) in the LWR were 3.30 for Puntius sophore, 3.22 for Puntius chola, 2.61 for Pethia ornatus and 3.27 for Pethia ticto. The relationships among TL, FL and SL were all linear (r2 > 0.95).
      PubDate: 2015-06-30T06:39:23.078395-05:
      DOI: 10.1111/jai.12841
  • Length–weight relationships of six fish species from the Zengqu
           River and the Ouqu River, southwest China
    • Authors: T. B. Zhu; D. G. Yang, Y. Liu, F. Li
      First page: 1153
      Abstract: This study provides the first report of length–weight relationships (LWRs) for six endemic fish species collected from the Zengqu River and the Ouqu River, two of the tributaries to the upper reaches of the Jinsha River, southwest China. Samples were obtained in May 2013 and September 2013 using various types of fishing gear. A total of 413 specimens belonging to six fish species were analyzed; their LWRs were W = 0.0069L3.086 (r2 = 0.987) for Schizothorax wangchiachii (Fang, 1936), W = 0.0076L3.029 (r2 = 0.980) for Schizothorax dolichonema (Herzenstein, 1889), W = 0.0029L3.315 (r2 = 0.961) for Schizopygopsis malacanthus (Herzenstein, 1891), W = 0.0082L 2.970 (r2 = 0.950) for Ptychobarbus kaznakovi (Nikolsky, 1903), W = 0.0017L3.464 (r2 = 0.957) for Triplophysa stolioczkae (Steindachner, 1866), and W = 0.0037L 3.190 (r2 = 0.956) for Triplophysa orientalis (Herzenstein, 1888). Prior to this study, the LWRs for S. dolichonema, P. kaznakovi and T. orientalis were unknown. In addition, new maximum sizes for four of the species are provided.
      PubDate: 2015-07-07T02:23:28.644231-05:
      DOI: 10.1111/jai.12843
  • Length–weight relationship of 13 fish species from the Ili River,
    • Authors: X. Y. Sui; X. Q. Li, H. Y. Sun, Y. F. Chen
      First page: 1155
      Abstract: Length‐weight relationships of 13 fish species are presented from samples collected in the Ili River, northwestern China. This study provides the first description of length‐weight relationships for five of the species. Coefficients of determination (r2) were >0.95 for 10 of the species.
      PubDate: 2015-08-04T07:51:03.636448-05:
      DOI: 10.1111/jai.12818
  • Length–weight relationships and reproductive parameters of Amblyceps
           apangi Nath and Dey, 1989 from Nagaland, India
    • Authors: L. N. Humtsoe; S. Bordoloi
      First page: 1158
      Abstract: The present study describes certain biological parameters of a hill stream catfish, Amblyceps apangi Nath and Dey, 1989 first described from Arunachal Pradesh, India. The distributional range of the fish was later recorded from the states of Manipur and Nagaland. The fish is very rare and this is the first report on certain biological parameters based on collections (140 males, 105 females) from streams in the Doyang watershed, a tributary of the Brahmaputra River. The value of the allometric coefficient (b) indicated close to isometric growth in males (2.989) and negative allometric growth in females (2.945). The relative condition factor was found to be close to 1 in different size groups. Both males and females attained first maturity (M50) in length group 6.0–7.0 cm. Peak values (Male 0.424, Female 1.465) in the gonadosomatic index (GSI) occurred from April to May, indicating one spawning season per year. The overall female to male ratio was 1 : 1.33. Absolute fecundity ranged from 17 to 122 for fishes of total length 6.34–10.3 cm.
      PubDate: 2015-07-07T02:19:30.942929-05:
      DOI: 10.1111/jai.12827
  • Length–weight relationships of two cyprinid fish species,
           Crossocheilus latius (Hamilton, 1822) and Garra gotyla gotyla (Gray, 1830)
           from the Ganga River Basin, India
    • Authors: N. K. Sharma; J. I. Mir, A. K. Dobriyal, R. Singh
      First page: 1161
      Abstract: This study provides length–weight relationship (LWR) information for two fish species (family Cyprinidae), Crossocheilus latius (Hamilton, 1822) and Garra gotyla gotyla (Gray, 1830), from a tributary of the Ganga River Basin, India. Both species had no previous LWR estimates as per Fishbase 2014.
      PubDate: 2015-06-23T02:03:17.034252-05:
      DOI: 10.1111/jai.12835
  • Length‐weight relationships of 10 fish species from two tributaries
           of Indus River Basin in Jammu and Kashmir, India
    • Authors: A. Bashir; N. K. Sharma, B. S. Bisht, R. Singh, J. I. Mir
      First page: 1163
      Abstract: Length‐weight relationships (LWRs) are described for 10 fish species belonging to three families from the Jhelum and Poonch River, tributaries of the Indus river basin in India. LWRs for these species were unknown to FishBase and new maximum lengths are recorded for three species. These results will be useful for fishery research, management and conservation in these tributaries of Jammu and Kashmir.
      PubDate: 2015-07-03T23:51:52.784768-05:
      DOI: 10.1111/jai.12844
  • Length–weight relationship of Hyporthodus haifensis and Mycteroperca
           rubra (Pisces; Serranidae) from the North‐Eastern Mediterranean Sea,
    • First page: 1165
      Abstract: Length–weight relationships (LWRs) for Hyporthodus haifensis and Mycteroperca rubra were studied using longlines at depths of 5–150 m, in the Gulf of Antalya, northeastern Mediterranean Sea. Fishing operations were carried out daily with 15 fishing boats. A total of 170 individuals were sampled. The LWR relationships of Hyporthodus haifensis and Mycteroperca rubra were calculated as W = 0.009L3.142 (r2) = 0.996 and W = 0.0081L3.0652 (r2) = 0.989 P 
      PubDate: 2015-06-23T01:37:44.131595-05:
      DOI: 10.1111/jai.12837
  • Length–length and length–weight relationships of fish species
           from the Lake Eyre Basin, Australia
    • Authors: D. Sternberg; B. Cockayne
      First page: 1168
      Abstract: The Lake Eyre Basin in central Australia is one of the world's last unregulated dryland river systems. To facilitate future research in this area, the relationships between total length, standard length, and wet weight for 10 widespread, large‐bodied freshwater fish species are presented. All linear relationships were strong (r2 > 0.90) and values of the exponent b of the length–weight relationships ranged from 2.786 to 3.336. These results provide novel baseline data for species in this area.
      PubDate: 2015-08-25T05:29:31.361148-05:
      DOI: 10.1111/jai.12863
  • Length–weight relationships for 85 fish species from Guam
    • Authors: K. T. Kamikawa; E. Cruz, T. E. Essington, J. Hospital, J. K. T. Brodziak, T. A. Branch
      First page: 1171
      Abstract: Length–weight relationships (LWRs) were developed for 85 reef and bottom‐fish species from Guam. These are the first published LWRs for Guam, and include new LWRs for nine species lacking these relationships in FishBase.
      PubDate: 2015-08-27T05:52:05.119635-05:
      DOI: 10.1111/jai.12877
  • Length–weight relationships of two fish species from the upper
           reaches of the Yangtze River, China
    • Authors: H. Wang; J. F. Tang, R. Ruan, F. Wang, W. Xiong
      First page: 1175
      Abstract: Length–weight relationships were determined for two fish species (Jinshaia sinensis Sauvage & Dabry de Thiersant, 1874; and Zacco platypus Temminck & Schlegel, 1846) collected from the upper reaches of the Yangtze River in China. The length–weight relationships were determined for the first time.
      PubDate: 2015-08-26T07:37:45.817067-05:
      DOI: 10.1111/jai.12876
  • Length–weight relationships for 13 fish species from a coastal
           artisanal fishery at Cape Verde peninsula (Senegal)
    • First page: 1177
      Abstract: Presented are length–weight relationships for 13 fish species captured by the coastal artisanal fishery at Yoff, a suburb close to Dakar (Senegal). Data were collected from April 2010 until March 2011. This work is thought to be the first reference of length–weight relationships for eight of the species. The b values estimated ranged from 2.51 to 3.47.
      PubDate: 2015-09-22T06:54:24.114184-05:
      DOI: 10.1111/jai.12890
  • Length‐weight relationships of five fish species from the Hongshui
           River, China
    • First page: 1180
      Abstract: The length‐weight relationships (LWRs) were determined for five Chinese endemic fish species caught by electrofishing, cast nets (mesh size 1 cm) and gillnets (mesh size 4 cm) in the Hongshui River during June and July 2011 and October to November 2012. These are the first LWRs records for these five little‐known species.
      PubDate: 2015-09-22T06:59:12.224802-05:
      DOI: 10.1111/jai.12896
  • Length–weight relationships and length at first maturity for nine
    • First page: 1182
      Abstract: Length–weight relationships are presented for nine fish species from the floodplain lakes in the Central Amazon (Amazon Basin, Brazil). The parameter slope b values in the length–weight relationships ranged from 2.33 to 3.28 for grouped sexes, and from 2.7 to 3.61 for separated sexes. Differences between sexes were verified in three species. Sizes at first sexual maturation ranged from 9.14 to 23.97. This study provides a new reference for the length–weight relationships of six species.
      PubDate: 2015-10-09T06:35:38.333307-05:
      DOI: 10.1111/jai.12919
  • A checklist of the protozoan and metazoan parasites of European eel
           (Anguilla anguilla): checklist of Anguilla anguilla parasites
    • Authors: E. Jakob; T. Walter, R. Hanel
      Abstract: A comprehensive literature review on prevalence and distribution of parasites of the European eel (Anguilla anguilla) in European waters was conducted to set a baseline for future trend analyses and biodiversity considerations. The resulting checklist compiled from peer reviewed journals as well as grey literature including scientific reports, conference contributions, PhD theses and own survey data includes all protozoan and metazoan parasites of the European eel from freshwater, brackish and marine habitats. Parasites are arranged according to phylum and family. Species within families are listed alphabetically. Infected host organs, recorded habitats, localities, countries and literature citations are provided for each species. A total of 161 parasite species/taxa from 30 countries are listed. With the trypanorhynch cestode Tentacularia coryphaenae a new host record could be documented. Furthermore, three new locality records of parasitic nematodes could be added based on own survey data: Anisakis simplex from an eel caught in the Baltic Sea, Eustrongylides mergorum for German freshwaters, and Goezia anguillae from the Tagus estuary in Portugal. Special consideration was given to the distribution of the swimbladder nematode Anguillcoloides (Anguillicola) crassus. A map and table of first reported records of this neozoan parasite species for 25 countries as well as a survey map indicating all published localities documented in the checklist are provided.
      PubDate: 2009-11-25T00:00:00-05:00
      DOI: 10.1111/j.1439-0426.2009.01345.x
  • Effects of tank colour on feeding, growth and stress responses of young
           taimen Hucho taimen (Pallas, 1773)
  • Length–weight and length–length relationships of 139
           Indo‐Pacific fish species (Teleostei) from the Davao Gulf,
    • Abstract: Length–weight relationships (LWRs) of 139 coral reef and pelagic fish species (representing 34 fish families) were calculated based on 3806 individuals measured at local fish markets near the Davao Gulf in the southern Philippines during weekly visits between March 2009 and July 2011, as well as in June 2012. Fishes were caught with a variety of fishing methods, corroborated by abrasions and injuries. Forty‐seven of 139 LWRs were firstly reported and new to science. The mean slope b of the LWRs was 3.035, indicating that the majority of studied species followed isometric growth. Standard length – total length relationships were calculated for all measured fish species. Additionally, standard length – fork length relationships are presented for 108 species. Moreover, fifteen new records of maximum fish length and weight are reported.
  • Length–weight relationships of seven fish species from the upper
           Pelus River, Kuala Kangsar district, Perak, Malaysia
    • Abstract: Length–weight relationships (LWR) are reported for seven fish species from the upper Pelus River, Kuala Kangsar district, Perak, Malaysia. This river is located in a remote area and supplies fishes for the aboriginal peoples. Fish samplings were conducted in three different seasons, the dry (June 2014), wet (August 2014), and moderately wet (April 2015). All samples were collected using fish electro–shocker and scoop nets with a 1 cm mesh size. A total of seven LWRs were unknown to FishBase, and four new maximum fish lengths were identified in the study.
  • Molecular characterization and expression of Kiss2/Kiss2r during embryonic
           and larval development in (Megalobrama amblycephala Yih, 1955)
    • Abstract: In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full‐length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real‐time PCR. Both Kiss and Kissr genes full‐length cDNA sequences of M. amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M. amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15 hpf stage and lowest in the 30 hpf /blastocyst stages for the embryonic period, highest in 7 dph/15 dph and lowest in 30 dph/30 dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species.
  • On the occurrence of the bluefin driftfish Psenes pellucidus Lütken
           1880 (Perciformes, Nomeidae) in Tunisian waters (Mediterranean Sea)
  • Strontium metabolism in the juvenile Lake Sturgeon, Acipenser fulvescens
           (Rafinesque, 1817), and further evaluation of the isotope as a marking
           tool for stock discrimination
    • Abstract: Stock enhancement efforts have been used to aid in increasing dwindling population levels of Lake Sturgeon, Acipenser fulvescens, that are currently of conservation concern. Consistent monitoring of stock enhanced fisheries requires a reliable and reproducible method to mark stocked fish such that the success of such programs can be determined. Recently, stable isotopic marking of hard structures in fish has been developed in a variety of species, however, optimal marking conditions are less well understood. In this study the metabolism of strontium in juvenile Lake Sturgeon and determined parameters for optimisation of marking success in the fin ray were examined. Using radioactive strontium (85Sr), whole body influx and efflux rates of strontium were 3.02 ± 0.364 pmol h−1 g−1 (mean ± standard error) and 0.04 ± 0.007 pmol h−1 g−1, respectively. Furthermore, short‐term accumulation of 85Sr in a variety of tissues was assessed and found to be greatest in the fin ray. Biological half‐life of the stable isotope 86Sr was shortest in muscle and longest in the fin ray tissue. Immersion and length of immersion timing had positive relationships with marking success. Additionally, the signature remained in 75% of fish analysed 550 days post‐mark. Data suggest that marking success was determined by duration of immersion in the mark and concentration of the mark introduced to the water.
  • Rapid visual assessment of spawning activity and associated habitat
           utilisation of sea lamprey (Petromyzon marinus Linnaeus, 1758) in a chalk
           stream: implications for conservation monitoring
  • Growth performance and body composition in response to dietary protein and
           lipid levels in Nile tilapia (Oreochromis niloticus Linnaeus, 1758)
           subjected to normal and temporally restricted feeding regimes
    • Abstract: A factorial experiment was designed to examine the effect on compensatory growth (CG) of Nile tilapia Oreochromis niloticus fed diets containing different protein and lipid levels under normal and temporally restricted feeding regimes. Four diets were formulated to contain either 30% or 36% crude protein, and 5% or 11% crude lipid. Triplicate replicates of each treatment were assigned to 24 150‐L tanks (20 fish/tank density). Fish (mean initial weight ± SD = 8.79 ± 0.34 g) were then fed either the normal feeding regime (thrice daily to apparent satiation) or the restricted regime (1 day feed deprivation followed by 3 days of feeding to apparent satiation) over a 44‐days study period. Fish receiving a diet under the restricted regime achieved weight gains (WG) comparable to fish consuming the diet containing 30% protein and 5% lipids under the normal feeding regime. Fish maintained on the restricted feeding regime exhibited reduced feed intake (FI), WG, feed efficiency ratio (FE), protein efficiency rate (PER) and hepatosomatic index versus fish on the normal feeding regime, except WG in fish fed the diet with 30% protein and 5% lipids. However, the resultant FI (85%~94%) was higher than the excepted 75% intake when fish were subjected to the restricted regime. Feeding 11% lipid diets led to improved FI, WG, FE, and PER compared to feeding the 5% lipid diets. Increased FI, WG, and FE, but reduced PER were observed in fish fed with 36% protein versus fish fed 30% protein. Fish receiving the 36% protein diets had lower whole‐body moisture and ash contents, but elevated whole‐body protein and lipid contents compared to those receiving the 30% protein diets. Whole‐body moisture contents were lower, but whole‐body protein, lipid and ash contents were higher in fish fed 11% lipid diets than in fish fed 5% lipid diets. There was an increase in whole‐body moisture content, but a decrease in protein and lipid content in response to the restricted feeding regime. Ash content was not affected by the feeding regime. The present study shows that Nile tilapia fed diets subjected to a restricted feeding regime exhibited growth comparable to those fed the diet at 30% protein and 5% lipid levels under a normal feeding regime. This positive effect was more pronounced in diets at a high protein level or in a combination of high protein and lipid levels.
  • Length–weight relationships of four freshwater cyprinid species from
           a tributary of Ganga River Basin in North India
    • Abstract: This paper provides length‐weight relationships (LWRs) for four freshwater cyprinid fish species, namely Barilius bendelisis (Hamilton, 1807), Bangana dero (Hamilton, 1807), Chagunius chagunio (Hamilton, 1807) and Labeo dyocheilus (McClelland, 1839) from the Kosi River, a tributary of the River Ganga in northern India. Altogether 848 specimens were caught bimonthly (for B. bendelisis) and quarterly for the other three species between March 2013 and December 2014 using cast nets (9 m length, 9 m width and 1/2 cm mesh size). LWRs for two of the four species (B. dero and C. chagunio) are recorded for the first time. The present study results will be useful for fishery researchers, management and conservation in this least‐explored tributary of the Ganga River Basin.
  • Length–weight and length–length relationships of three
           cyprinid fishes from the Bibi‐Sayyedan River, western Iran
    • Abstract: This study describes the length–weight and length–length relationships for three cyprinid species from the Bibi‐Sayyedan River (western Isfahan provinces, Iran). The slope parameter (b) values in the length–weight relationship equations were determined as 3.0729 for Alburnus mossulensis Heckel, 1843; 2.8509 for Barbus lacerta Heckel, 1843; and 3.0864 for Chondrostoma regium (Heckel, 1843). This study presents the first LWR and LLR references for these species in the Bibi‐Sayyedan River.
  • Effects of salinity on growth, body composition, muscle fatty acid
           composition, and antioxidant status of juvenile Nile tilapia Oreochromis
           niloticus (Linnaeus, 1758)
  • Relationships between fish size and otolith measurements for Chlorurus
           sordidus (Forsskål, 1775) and Hipposcarus harid (Forsskål, 1775)
           from the Red Sea coast of Egypt
  • Length–weight relationships of three fish species from the upper
           reaches of the Beijiang River, Shaoguan, China
    • Abstract: Fish assemblages were randomly collected in March 2015 from the upper reaches of the Beijiang River, Shaoguan, China. Fish were sampled on 10 occasions using gill nets (mesh size: 3 cm) and the length–weight relationships (LWRs; W = a × Lb) of three native species recorded: Xenocypris davidi, Xenocypris macrolepis and Culter recurviceps. The exponent b varied from 2.803 to 3.096 and r2 values ranged 0.985–0.992. This is the first report on the LWRs of fish from this area, with valuable results for the research, management and conservation of fishery resources in the upper reaches of the Beijiang River.
  • Length–weight relationships for common juvenile fishes and prey
           species in Hawaiian estuaries
    • Abstract: Length–weight relationships (LWR) using 3876 individuals were determined for 39 species sampled in estuarine systems in the main Hawaiian Islands, including 33 species of fish from 21 families, five species of crustaceans (prey) from two families, and one mollusk species. For 17 species, LWRs were estimated for the first time. For another 14 species their LWRs were newly reported for Hawaiian waters, including seven introduced taxa that are well established in the islands' estuaries. The LWRs for all taxa include juvenile and sub‐adult size ranges commonly encountered in Hawaiian estuaries.
  • Application of data‐limited assessment methods on black anglerfish
           (Lophius budegassa, Spinola, 1807) stocks in the Mediterranean Sea
    • Abstract: A survey‐based assessment of an eastern Mediterranean data‐limited black anglerfish (Lophius budegassa; Spinola, 1807) stock was carried out to elucidate its population and exploitation trends. A catch‐based method was also applied to estimate its maximum sustainable yield (MSY). The effect on the long‐term spawning stock biomass and yield of a wide range of exploitation regimes (combinations of F and selectivity) was investigated using an age‐structured population model parameterised for Mediterranean anglerfish stocks. The analysis indicated an increasing trend of anglerfish fishing mortality (F) in the eastern Mediterranean from the mid‐1990s onwards, and that recent catches were 41% higher than the median MSY estimate. Catching Mediterranean anglerfish more than 3 years after they mature at an F = 0.4–1 year−1 would ensure high yields at sustainable levels of stock depletion. Examination of the empirical exploitation regimes in five anglerfish stocks across the Mediterranean Sea illustrates their unfulfilled potential for higher sustainable yields, mainly due to overexploitation of juveniles.
  • The distribution, diversity and abundance of elasmobranch fishes in a
           modified subtropical estuarine system in Kuwait
    • Abstract: To address the paucity of information on the spatial and temporal composition of elasmobranch fish assemblages in the Arabian region, data from fisheries surveys around Kuwait's Boubyan Island, in the Tigris‐Euphrates‐Karun delta at the head of the Arabian (Persian) Gulf were examined. A total number of 461 elasmobranchs consisting of at least 13 taxa were captured by gillnets (300 m length, 4, 4.5, 5.5, and 5.75 inch stretch mesh) and otter trawls (5‐m chain line length, 34 and 10 mm stretch mesh in body and cod end, respectively) from February 2004 to March 2005. Seventy‐nine gillnet sets averaged 24 min soak time, while 431 5‐min trawl tows averaged 4.1 km h−1 for a swept area of 1025 m2 tow−1 with a net spread of 3 m. Most taxa were infrequently recorded. Of the six species that accounted for over 90% of individual abundance, the longheaded eagle ray Aetobatus flagellum (170, 269.5 kg) and the scaly whipray Himantura imbricata (137, 17.3 kg) ranked first and second, respectively. Most sharks were Arabian carpetshark Chiloscyllium arabicum (22, 7.7 kg) and milk shark Rhizoprionodon acutus (21, 2.3 kg). Species richness for gillnets was greatest along the main Gulf coast (H' = 1.87), although H. imbricata and A. flagellum were widespread. Based on published birth sizes, neonates or young‐of‐year of several species were recorded. Results indicated that the water temperature strongly determined seasonal distribution: no elasmobranchs were recorded in the winter following a 14°C drop after October, and diversity, abundance and biomass generally increased in summer. While salinity varied from 28.4 to 39.8, major anthropogenic modifications in the upper Tigris‐Euphrates watersheds and development of Boubyan Port will result in higher salinities, which in turn will influence longer‐term patterns of elasmobranch distribution.
  • Investigations on the reproductive biology and diet of yellowfin tuna,
           Thunnus albacares, (Bonnaterre, 1788) in the Oman Sea
    • Abstract: In this paper, the examination of some reproductive features and stomach contents of yellowfin tuna, Thunnus albacares, were determined by samples taken from Iranian drifting gillnets in the Oman Sea in the years 2007 to 2009. The male to female ratio in overall size was 1 : 0.93, which was not significantly different from the expected value 1 : 1. Fish lengths ranged from 37 to 152 cm, averaging 78.5 cm for females and 79.2 cm for males. At a larger size (>117 cm) males were proportionally predominant. Length at first maturity, Lm 50%, was observed when females reached 77.2 cm. Data from maturity stages indicated a single spawning period, peaking in May‐June, corresponding with a drop in the Gonadosomatic Index (GSI). Fishes were the main prey group, comprising 47.6% of total numbers among the three food item categories in the tuna stomachs. Among the 12 families identified in the stomachs, the Portunidae swimming crab crustacean was the most important prey organism (18.7% of total numbers). A wide range of prey species found in yellowfin tuna stomachs reflects an opportunistic feeding behaviour restricted by local prey availability. Considering the scarcity of available data, the results of the present paper will provide a reference for better knowledge of the biological features of T. albacares in the Oman Sea.
  • Length–weight relationships of 45 fish species in the Min River
           Estuary, East China Sea
    • Abstract: Forty‐five fish species belonging to 35 genera from 22 families and 10 orders were collected during 2006–2007 by otter trawl in the Min River Estuary, East China Sea, at depths of 10–50 m, for analysis of their length–weight relationships (LWRs). Analyses included 20 species for which no previous LWR information was available in FishBase.
  • Experimental evaluation of calcein and alizarin red S for immersion
           marking of silver carp Hypophthalmichthys molitrix (Valenciennes, 1844)
    • Abstract: Calcein (CAL) and alizarin red S (ARS) at concentrations of 50–200 and 150–300 mg/L, respectively, were used for immersion marking of juvenile silver carp Hypophthalmichthys molitrix (79.65 ± 2.11 mm total length, mean ± SD). The marked fish were kept in separate tanks (0.012 individuals per litre, rearing temperature 18.4–25.7°C) for 360 days. Each experimental treatment group consisted of three replicates (16 individuals per replicate). Immersion for 24 h produced detectable marks in the sagittae, lateral line and non‐lateral line scales, and fin rays (dorsal, pectoral, ventral, anal, and caudal) at 360 days post‐marking. Acceptable marks in the sagittae were observed for CAL at concentrations of 150–200 mg/L and for ARS at concentrations of 200–300 mg/L. Fluorescent marks were poorly visible in all non‐lateral line scales from both the CAL‐ and ARS‐treated groups. Acceptable fluorescent marks in the lateral line scales and fin rays were detected for CAL at concentrations of 150–200 and 100–200 mg/L, respectively, and for ARS at concentrations of 200–300 and 150–300 mg/L, respectively. In particular, optimal marks were observed at the highest concentrations investigated in lateral line scales (200 mg/L CAL, 300 mg/L ARS) and fin rays (200 mg/L CAL, 200–300 mg/L ARS). There was no significant difference in the survival or growth of marked fish compared to controls throughout the experiment (P > 0.05).
  • Length–weight and length–length relationships for three
           endemic Pseudophoxinus species in Turkey
    • Abstract: Length–weight and length–length relationships are presented for three endemic Pseudophoxinus species in Turkey. This study provides the first references for these relationships in the species examined.
  • Length–weight relationships for five fish species from Jingpo Lake,
           Heilongjiang, China
    • Abstract: In this study the length–weight relationships for five fish species from Jingpo Lake in China belonging to three families and five genera were analyzed. A total of 368 specimens were used to estimate the relationship parameters. One species (Leuciscus waleckii) had no previous LWR estimate in FishBase.
  • The first description of oarfish (Regalecus russellii Cuvier 1816)
           (Regalecidae) ageing structures
  • A morphometric investigation of the small yellow croaker (Larimichthys
           polyactis Bleeker, 1877): evidence for subpopulations on the Chinese coast
    • Abstract: In this study the morphometric characteristics based on the truss network system were constructed from the body shapes and used to investigate the population structure of the small yellow croaker (Larimichthys polyactis Bleeker, 1877). From April to June 2013, a total of 431 fish samples were collected from commercial catches acquired with bottom trawlers (20 × 20 mm) in seven main spawning grounds along the coast of China. The results of principal component and discriminant function analyses indicated a clear distinction among the regional stocks and within the Haizhou Bay (HZB) stock. Observed differences primarily involved head measurements, body length and tail size, which were associated with environmental factors. Latitudinal gradient of the stock distribution and the relationship among the stocks provided further evidence for the existence of subpopulations and the re‐division of the East China Sea group. Moreover, the temporal inter‐structure of the HZB stock is a potential reason for the contrast in results from previous studies. Additional thorough studies of migration trajectories are required for a conclusive and improved understanding of the population structure.
  • Comparative study of the circulatory system of common carp (Cyprinus
           carpio Linnaeus, 1758) and beluga (Huso huso Linnaeus, 1758): a corrosion
           cast study
    • Abstract: The present work is the comparative study of the circulatory system in common carp (Cyprinus carpio Linnaeus, 1758) and beluga (Huso huso Linnaeus, 1758) with emphasis on the heart and main blood vessels, employing the corrosion cast methodology. A thorough comparative study of the circulation system would be useful for further anatomical, physiological and pathological studies. Ten 3000 g H. huso and ten 2000 g C. carpio from a commercial fish farm were kept at 18 and 24°C, respectively, and fed commercial food. For the experiment, fish were anaesthetized then killed using an overdose of benzocain solution in ethanol; subsequent attempts were made to fill the heart and blood vessels with artificial resin fluid made on the basis of methylmetacrylate. The specimens were submerged in a waterbath (20–24°C) until polymerization of the methylmetacrylate was complete. The maceration stage was performed in a 25% solution of KOH for 24–48 h to dissolve the surrounding tissues. Finally, various parts of the heart and blood vessels were retained in their natural positions, thereby demonstrating the anatomical details of the circulatory system. The main elements such as the sinus venosus, atrium, ventricle, bulbus and conus arteriosus, dorsal aorta, subclavian artery, coelo‐mesentric artery, renal artery, caudal aorta, hepatic vein, common cardinal vein and related smaller vessels were visualized to the extent that these elements could be used to represent the similarities and variations between different species in comparative anatomy but also as a description of the normal features. These can be compared to pathological situations in fish disease studies, as both species are important aquaculture candidates.
  • Limited influence of a wind power project submarine cable on a Laurentian
           Great Lakes fish community
    • Abstract: Previous research has identified the generation of electromagnetic fields (EMFs) emanating from renewable energy project transmission cables to be a potential stressor to aquatic communities. In this study, we investigated whether the presence of a high voltage submarine transmission cable affected the spatial pattern and composition of nearshore and offshore fishes at a Laurentian Great Lakes site. The transmission cable investigated in this study runs 7.8 km along the lakebed of Lake Ontario, carrying electricity from the Wolfe Island wind power project to the city of Kingston, Ontario. In autumn of 2011, both nearshore electrofishing and deeper‐water fisheries acoustic surveys were conducted along transects at varying distances to the cable. For both habitat types, no detectable effects of the cable on the fish community were found. Local habitat variables, including substrate or depth, were more important in explaining variation in fish density than proximity to the cable. Common species encountered during the surveys were round goby (Neogobius melanostomus) in the nearshore and alewife (Alosa pseudoharengus) in the deeper channel. American eel (Anguilla rostrata), thought to be an electromagnetically sensitive species, was also encountered during the surveys including in close proximity to the cable. More robust impact assessments require sampling fishes before a cable installation, over greater time frames (additional seasons or years), and habitats that support more diverse native assemblages.
  • Age and growth of bream Abramis brama (Linnaeus, 1758) in the downstream
           section of Irtysh River in China
  • Comparison of inshore and offshore fish larval assemblages within the
           wider Yangtze River estuary (China)
    • Abstract: It is hypothesized that fish larvae undertake vertical movements in estuarine waters. To test this hypothesis, a sampling period was selected during times when many spring‐spawning fish reach maximum abundance in coastal waters so that spring and summer variations in larval fish assemblages could be determined in the Yangtze River estuary. Six oceanographic surveys were conducted across the salinity gradient of an inshore (freshwater) and offshore area (brackish intersection) during spring (May) and summer (August) between 2010 and 2012. The fish larval community was dominated by species of Engraulidae, Gobiidae, Champsodontidae and Mugilidae. The pre‐flexion and flexion larval stages of euryhaline marine species, which are dependent on estuaries as nursery areas, were common. The brackish and marine larval assemblage was the most abundant with taxa such as Coilia mystus and Engraulis japonicus accounting for more than 57.3% of the total catch. Spatial differences in the taxonomic composition of larval fish assemblages were evident between the inshore and offshore areas. Additionally, the Yangtze River runoff regulatory functions as affected by the Three Gorges Reservoir operational mode (hydrological alternating operations) showed weak influences on fish and habitat environments. Low salinities from high freshwater inflow limited bay anchovy production in the inshore area.
  • New data on the longevity of coastal cod Gadus morhua Linnaeus, 1758 in
           the White Sea
    • Abstract: The cod, Gadus morhua, is a common and abundant demersal fish in the White Sea coastal zone. The published data on the maximum age of White Sea cod still requires additional documentation, because some authors have noted difficulties in age determination by otoliths of large specimens. To obtain accurate data on the longevity of this species in the White Sea, an image analysis of thin‐sections of otoliths was made for age estimation. Research surveys in the Chupa Inlet and adjacent waters of the Kandalaksha Bay were conducted in June to August from 2007 to 2013. The five largest specimens of a total of 3564 captured fish were selected for age determination. Age of these largest individuals ranged between 7 and 12 years, total length and weight varied from 60.2 to 77.0 cm and from 2.4 to 6.1 kg, respectively. According to the data, maximal White Sea cod longevity is greater than in previously published data, and demonstrates similar longevity to conspecifics from the Baltic and North seas, whereas its life cycle is much shorter than cod from the Northeast Arctic, Iceland, Greenland, Newfoundland and Labrador stocks.
  • Length‐weight relationships of five fishes from the middle
           Heilongjiang River, China
    • Abstract: Length–weight relationships (LWRs) were estimated for five fish species in the Fuyuan reach of the middle reaches of Heilongjiang River, northeast China. Fishes were captured using a variety of nets (i.e. drift gill net, set net, fishing cage, fishing jig and fishhook). In addition to November–April, samples were collected monthly from May 2013 to October 2014. The five species were: Lethenteron reissneri, Pelteobagrus ussuriensis, Hemibarbus maculates, Xenocypris macrolepis and Chanodichthys erythropterus. LWRs for Lethenteron reissneri and Pelteobagrus ussuriensis were unknown to FishBase. The a values obtained ranged from 0.0019 (Lethenteron reissneri) to 0.0177 (Pelteobagrus ussuriensis), and b values ranged from 2.710 (Pelteobagrus ussuriensis) to 3.397 (Chanodichthys erythropterus).
  • High stocking density during larviculture and effect of size and diet on
           production of juvenile Lophiosilurus alexandri Steindachner, 1876
           (Siluriformes: Pseudopimelodidae)
    • Abstract: Different stocking densities were investigated in larviculture and feeding of Lophiosilurus alexandri, as well as analyses of the effects on juveniles of two size‐classes and two different commercial formulated diets. The first experiment was two‐phased: (a) larvae stocked at densities of 60, 120, 180, 240, and 300 larvae L−1 fed with Artemia nauplii and reared for 15 days; (b) in phase 2, densities of 5, 10, 15, 30, and 40 juveniles L−1 were evaluated during feed training (20 days). Mean water temperature in both phases was 28°C. In the first phase of experiment 1, the different stocking densities did not affect fish growth or survival. In phase 2, growth was similar in all densities; however, survival was lower at higher densities. The increased density provided a rise in biomass and number of individuals produced in both phases. In the second experiment, two size‐classes of feed‐trained juveniles (30.22 ± 1.84 and 34.66 ± 2.41 mm) were given pellets of two different diameters (1.2 and 2.6 mm) for 20 days. The largest juveniles fed the 1.2 mm inert diet had higher final weights and lengths. Larviculture and feed training of L. alexandri can thus be performed successfully at high stocking densities of 300 larvae L−1 during the first 15 days of feeding, and at densities of up to 40 juveniles L−1 during the 20 days of feed training, respectively.
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