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Journal Cover Journal of Applied Ichthyology
  [SJR: 0.482]   [H-I: 36]   [6 followers]  Follow
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 0175-8659 - ISSN (Online) 1439-0426
   Published by John Wiley and Sons Homepage  [1598 journals]
  • Effect of cellulase, phytase and pectinase supplementation on growth
           performance and nutrient digestibility of rainbow trout (Oncorhynchus
           mykiss, Walbaum 1792) fry fed diets containing canola meal
    • Authors: N. O. Yigit; E. Keser
      Abstract: This experiment was conducted to evaluate the effects of supplementing exogenous enzymes on growth, feed conversion ratio (FCR) and apparent nutrient digestibility in rainbow trout (Oncorhynchus mykiss) fry diets containing 32% canola meal. Five experimental diets (including a control diet containing no enzymes) were prepared as isonitrogenous (44% crude protein) and isocaloric (4000 kcal DE kg1). The four other diets contained either cellulase, phytase, pectinase or an enzyme mix (a mixture of cellulase, phytase and pectinase in the same ratio). The feeding trial was conducted in triplicate for 12 weeks in 15 tanks (100‐L). At the beginning of the experiment 20 rainbow trout fry (initial weight 1.23 g) were stocked into each tank. Mean water temperature in the rearing tanks was 11°C and water flow in each tank was 6 L min−1. At the end of the experiment the growth parameters and FCR displayed no significant differences in enzyme supplementation (P > 0.05). In addition, no differences were observed in dry matter, protein, or lipid digestibility with enzyme supplementation (P > 0.05). The results of this study showed that the addition of pectinase, phytase, cellulase or an enzyme mix to a diet containing 32% canola meal had no effect on growth, feed efficiency or dry matter, protein, or lipid digestibility in rainbow trout fry.
      PubDate: 2016-04-15T07:14:24.909273-05:
      DOI: 10.1111/jai.13088
  • Growth, parasitic infection and hematology in Colossoma macropomum Cuvier,
           1818 fed diets containing Allium sativum
    • Abstract: The aim of this study was to investigate the effects of a dietary Allium sativum (garlic) addition on growth, parasite infestation and blood parameters in Colossoma macropomum (tambaqui) using 12 net‐cages (1 m3). There were four treatments with three replicates each (15 fish per replicate). Tambaqui of 112.4 g mean weight were fed for 45 days with diets containing 0, 15, 30 and 45 g of fresh garlic kg−1 feed. Garlic did not affect the tambaqui survival, growth, feed conversion, body condition, plasma glucose levels, total plasma protein levels or red blood cells. Total leukocyte and lymphocyte numbers decreased in fish fed diets containing 45 g garlic kg−1 feed, but neutrophils decreased in fish fed diets containing 15, 30 and 45 g garlic kg−1 diet, while the number of immature leukocytes increased in fish fed 30 g and 45 g garlic kg−1diet. Gills in fish fed 30 and 45 g kg−1 diet had lower infestation (52% and 63%, respectively) of Anacanthorus spathulatus (Monogenea) compared to controls. The application of garlic in diets improved the response of leukocytes and enhanced the resistance to infections by monogeneans.
      PubDate: 2016-04-15T07:14:02.225047-05:
      DOI: 10.1111/jai.13086
  • A new record of dasyatid fish from the Sea of Marmara: Tortonese's
           stingray, Dasyatis tortonesei Capapé, 1975 (Dasyatidae)
    • PubDate: 2016-04-15T07:13:39.377548-05:
      DOI: 10.1111/jai.13087
  • Ovarian development of albacore (Thunnus alalunga Bonnaterre, 1788) in the
           Eastern Mediterranean Sea: a histological characterisation
    • Authors: F. S. Karakulak; T. Akayli, I. K. Oray, R. E. Yardimci
      Abstract: In order to investigate the stages of oogenesis in the albacore Thunnus alalunga, 129 females (64.0‒83.5 cm fork length‐FL) were captured by gill nets from April to August 2002–2008, in the eastern Mediterranean Sea. Based on histological examination of the ovaries, a total of seven ovarian developmental stages were identified: perinucleolar, lipid, early vitellogenesis, late vitellogenesis, migratory nucleus, pre‐hydrated and hydrated. The monthly gonadosomatic index values and the results of the histological analysis indicated that the females mature in May. The spawning stage becomes predominant in June and reaches a maximum in July. The evidence reported here indicates that the size of females at first sexual maturity was lower for the Mediterranean albacore stock than for the Pacific and Atlantic stocks.
      PubDate: 2016-04-15T07:13:30.967876-05:
      DOI: 10.1111/jai.13076
  • Trapped in their own ‘home’: unexpected records of intertidal
           fish desiccation during low tides
    • PubDate: 2016-04-15T07:12:38.959231-05:
      DOI: 10.1111/jai.13074
  • Length–weight relationships and condition factors of two fish
           species from the southern Caspian Sea basin: Alburnoides samiii
           Mousavi‐Sabet, Vatandoust & Doadrio, 2015 and Ponticola iranicus
           Vasil′eva, Mousavi‐Sabet & Vasil′ev, 2015
    • Abstract: The present study describes the length–weight relationships (LWRs) and condition factors (K) of Alburnoides samiii and Ponticola iranicus in the Sefidroud River from the southern Caspian Sea basin, September 2013 to August 2014. This study presents the first known reference in the FishBase database regarding the LWRs and condition factors for these two indigenous species.
      PubDate: 2016-04-15T07:12:12.543259-05:
      DOI: 10.1111/jai.13083
  • Length‐weight relationships of three cyprinid fish species from
           southwestern China
    • Authors: H. Y. Cao; Z. Yang, L. Cai, L. Pan
      Abstract: Length–weight relationships (LWRs) were determined for three cyprinid fish species [Acrossocheilus yunnanensis (Regan, 1904), Onychostoma simum (Sauvage & Dabry de Thiersant, 1874) and Spinibarbus sinensis (Bleeker, 1871)] from southwestern China. Samples were obtained with various fishing gear (set nets, drift gill nets, fish cages, hook and electrofishing). Prior to this study, the LWRs for these three species (A. yunnanensis, O. simum and S. sinensis) were unknown.
      PubDate: 2016-04-15T07:12:00.441087-05:
      DOI: 10.1111/jai.13085
  • Morphological divergence in Indian oil sardine, Sardinella longiceps
           Valenciennes, 1847– Does it imply adaptive variation?
    • Authors: S. Sukumaran; A. Gopalakrishnan, W. Sebastian, P. Vijayagopal, S. Nandakumar Rao, N. Raju, S. Ismail, E. M. Abdussamad, P.K. Asokan, K. P. Said Koya, P. Rohit
      Abstract: The Indian oil sardine, Sardinella longiceps, is an important pelagic species in Indian waters, and shows divergent morphology while in sympatry. The reasons behind this divergent morphology were investigated using morphometric, genetic and nutritional analyses. Twenty‐one morphometric characters (as percentage of standard length) and eight meristic characters were studied in the three variants to assess whether they are significantly diverged. Distinct clustering of morphotypes was evident in the principal component analysis on log‐transformed ratios of morphological characters with PC1 and PC2, explaining 50.7% and 17.6% of the total morphological variation, respectively. PC1 was highly correlated with the distance from snout to anal origin, depth at dorsal, distance from snout to pelvic and distance from snout to first dorsal. PC2 was highly correlated with head length, caudal width and anal depth. Analysis of similarities (ANOSIM) was conducted using log‐transformed morphometric ratios, with the results showing the clusters to be well differentiated (R = 0.511; P 
      PubDate: 2016-04-15T07:11:57.252282-05:
      DOI: 10.1111/jai.13060
  • Isolation and characterization of ten polymorphic microsatellite loci from
           Gymnocypris chui and cross‐amplification in seven highly specialized
           Schizothoracinae fishes
    • Authors: R. Y. Zhang; K. Zhao
      PubDate: 2016-04-15T07:11:03.238099-05:
      DOI: 10.1111/jai.13066
  • Length–weight relationship of 10 freshwater fish species in
           Amazonian streams, Trombetas River basin (Brazil)
    • Authors: D. C. O. Rosa; B. E. Soares, M. P. Albrecht, E. P. Caramaschi
      Abstract: Length–weight relationships (LWR) were analyzed for 10 fish species from 26 Amazonian streams located in four micro‐basins in the Trombetas River basin, Pará State, Brazil. LWRs were calculated based on 1831 individuals sampled between 2010 and 2012 using a manual trawl. The coefficients of proportionality and allometry were within the ranges estimated for most fishes. No previous LWR information was available for these species in the literature.
      PubDate: 2016-04-07T23:11:50.168845-05:
      DOI: 10.1111/jai.13082
  • The ontogeny of neurosensory structures in larval Atlantic halibut,
           Hippoglossus hippoglossus (Linnaeus, 1758)
    • Authors: A. J. Borsky; I. R. Bricknell
      Abstract: The ontogenic development of neurosensory structures in larval Atlantic halibut, Hippoglossus hippoglossus (L.) was examined using microscopy imaging techniques and analysis. Larval samples were preserved at developmental stages of 444, 486, 528, 570, 612, and 666 degree‐days, respectively. Larvae were measured and photographed macroscopically. The fish were then embedded in paraffin for histological processing. 5 μm sequential sections of the anterior of the larvae were cut and stained using hematoxylin and eosin dyes. The resulting sections were examined using an Olympus BX60 compound microscope. The development of neurosensory structures such as neuromasts and sensory papillae were observed throughout all developmental stages of larvae and early development of the lateral line was observed at 666 degree‐days. Neuromast frequency increased as larvae matured, suggesting that larvae were approaching their first feeding event towards the end of the sampling period. Possible implications of development on larval first feeding are discussed.
      PubDate: 2016-04-07T01:41:40.94088-05:0
      DOI: 10.1111/jai.13064
  • Occurrence of chromatophoroma or chromatophoromatosis in Sardinella
           longiceps (Valenciennes, 1847) from Tamilnadu, southeast coast of India
    • Authors: V. Singaravel; A. Gopalakrishnan, R. Vijayakumar, K. Raja, S. Asrafuzzaman
      Abstract: The study was undertaken to analyse the prevalence of skin tumours in Sardinella longiceps, and to investigate the tumour type occurrence, distribution and histology. Fish samples were collected from three different landing centres (Cuddalore, Parangipettai and Nagapattinam) of Tamilnadu, southeast coast of India, from January 2014 to December 2014. A total of 192 661 individuals were examined, 750 of which had tumours for an overall prevalence of 0.39%. By station, higher prevalence was observed in Parangipettai (0.41%) followed by Cuddalore (0.39%) and Nagapattinam (0.36%). Chromatophoromas were predominant among the types of tumours in sardines. The multi‐coloured cutaneous tumour masses were in the head and body skin. Among the chromatphoromas, the erythrophoromas were most abundant followed by melanophoromas, iridophoromas and leucophoromas. Histologically, the tumour lesions were characterized by hyperplastic pigment cells intermingled with fibroblasts. The gross‐morphological and histopathological diagnoses of these lesions were compatible with erythrophoroma, melanophoroma, iridophoroma and leucophoroma.
      PubDate: 2016-04-07T01:41:18.148641-05:
      DOI: 10.1111/jai.13084
  • Diet composition of greater weever, Trachinus draco (Linnaeus, 1758)
           captured in the eastern‐central Adriatic Sea in relation to fish
           size, season and sampling area
    • Abstract: The diet compositions of greater weever, Trachinus draco, from the eastern‐central Adriatic Sea, were investigated with respect to seasons, fish size and sampling locations. Analyzed were the stomach contents of 592 specimens, total length (TL) of 9.9–31.2 cm, collected by commercial bottom trawls from January to December 2008. The frequency of empty stomachs varied significantly with the season (43.3% maximum during winter; 6.8% minimum during summer). Prey items identified in the stomachs belonged to seven major taxonomic groups: Bivalvia, Cephalopoda, Decapoda, Mysidacea, Isopoda, Amphipoda and Teleostei. Decapods were the most important prey (%IRI = 72.8) followed by teleosts (%IRI = 16.4) and mysids (%IRI = 15.0); other prey groups were only occasionally ingested. Diet composition showed little seasonal variation; decapods were the most important prey in all seasons. Fish size was the most important factor influencing the composition of the diet. Small individuals (
      PubDate: 2016-04-07T01:40:52.425632-05:
      DOI: 10.1111/jai.13081
  • The effects of endosulfan on P450 1A gene expression, antioxidant enzymes
           activity and histopathological alterations in liver of Persian sturgeon
           (Acipenser persicus Borodin, 1987)
    • Authors: R. Safari; M. Khalili, M. Reza Imanpour, M. Pourkazemi
      Abstract: The effects of 14 days exposure to sublethal concentrations of endosulfan (10 and 40 μg L−1) were investigated in mRNA‐ P450 1A expression, antioxidant enzymes (SOD and CAT) activity and histopathological alterations of Persian sturgeon (Acipenser persicus) fingerlings with weights of 3–5 g. The results illustrated that the relative mRNA‐ P450 1A expression level significantly increased (P 
      PubDate: 2016-04-06T03:25:20.12785-05:0
      DOI: 10.1111/jai.13072
  • Length–weight relationships of two fish species from the middle
           reaches of the Jinsha River, China
    • Authors: T. B. Zhu; D. G. Yang
      Abstract: This study provides the first report of length–weight relationships (LWRs) for Pseudogyrinocheilus prochilus (Sauvage & Dabry de Thiersant, 1874) and Jinshaia sinensis (Sauvage & Dabry de Thiersant, 1874) collected from the middle reaches of the Jinsha River, southwest China. Samples were obtained between June 2005 and November 2006. A total of 491 specimens belonging to two fish species were analyzed. LWRs were W = 0.0170L2.970 (r2 = 0.980) for P. prochilus and W = 0.0194L2.849 (r2 = 0.977) for J. sinensis. Prior to this study, the LWRs for P. prochilus were unknown. In addition, new maximum size for J. sinensis was provided.
      PubDate: 2016-04-06T03:19:05.334064-05:
      DOI: 10.1111/jai.13079
  • Patterns of reproduction and growth of the catfish Iheringichthys labrosus
           (Lütken, 1874) after a reservoir formation
    • Authors: H. Soares de Santana; A. Cantarute Rodrigues, C. Dei Tos
      Abstract: This work contributes to the knowledge of the reproduction, growth parameters and mortality rates of Iheringichthys labrosus in a newly‐formed reservoir. A total of 554 males and 1227 females were collected over 12 consecutive months, 1998–1999, from sites in the Corumbá Reservoir, Brazil, using gillnets (meshes: 2.4–16 cm). Information on each individual, i.e. standard length (cm), weight (g), sex, and gonadal development phase was recorded. The pectoral spines were removed to estimate age. The number of juveniles and adults, males and females, reproductive sites and seasons were estimated. First maturation length was estimated using a likelihood function fitted by binomial distribution. Growth parameters were estimated using the von Bertalanffy equation. Total instantaneous mortality was obtained through a linearized catch curve method. Standard length varied from 6.0 to 20.5 cm. Growth showed negative allometry for both sexes. The reproduction period was August to December in all environments sampled and first maturation length was 11.5 cm. All individuals were adults with 17.0 cm standard length. Ages varied from zero to 7 years. Asymptotic length, growth coefficient and t0 for the entire population were 27.79 cm, 0.12 and −2.64, respectively. Instantaneous and annual mortality rates were 0.90 and 0.59, respectively.
      PubDate: 2016-04-02T02:42:10.11435-05:0
      DOI: 10.1111/jai.13059
  • Cold storage effects on flesh quality of rainbow trout Oncorhynchus mykiss
           (Walbaum, 1792) fed diets containing different vegetable oils
    • Authors: M. Yildiz; I. Kose, G. Issa, T. Kahraman, E. Guven, M. A. Baltaci, K. Yuruten
      Abstract: The intention of this experiment was to assess the effects of different sources of dietary lipid on the fatty acid composition of the fillet and liver and the flesh quality traits of rainbow trout (Oncorhynchus mykiss) after a 70‐day feeding period. Four iso‐nitrogenous (approx. 51% crude protein) and iso‐lipidic (approx. 14% crude lipid) experimental diets were formulated. The control diet contained only fish oil (FO) as the primary lipid source. In the other three dietary treatments, fish oil was replaced by 100% (LO30/SO35/SFO35) and 70% (FO30/LO35/SO35 or FO30/SO35/SFO35) sesame oil (SO), linseed oil (LO), or sunflower oil (SFO). Triplicate groups of 40 rainbow trout (~46 g) held under similar culture conditions were hand‐fed daily to apparent satiation for 70 days. At the end of the feeding trials, no difference in growth performance among experimental groups was noted (P > 0.05). There were some differences in the proximate composition of fish fillets (P 
      PubDate: 2016-04-02T02:41:43.972855-05:
      DOI: 10.1111/jai.13037
  • Elasmobranch captures in shrimps trammel net fishery off the Gulf of
           Gabès (Southern Tunisia, Mediterranean Sea)
    • Authors: B. Saidi; S. Enajjar, M. N. Bradai
      Abstract: Small‐scale fisheries are generally promoted as a sustainable alternative to large‐scale industrial fisheries. However, there is recent growing evidence that small‐scale fisheries may be the largest threat to marine species of conservation concern. The objective of this study was to evaluate the potential impact of the trammel net fishery on elasmobranchs in the Gulf of Gabès, Southern Tunisia. Data are based on 191 shrimp trammel net set (40 mm stretched mesh size) surveys conducted aboard commercial fishing vessels from May to July 2009. Five species of the small coastal elasmobranchs (Mustelus mustelus (Linnaeus, 1758), Mustelus punctulatus Risso 1827, Dasyatis pastinaca (Linnaeus, 1758), Dasyatis marmorata (Steindachner, 1892) and Torpedo torpedo (Linnaeus, 1758)) and two species from the large coastal shark (Carcharhinus plumbeus (Nardo, 1827) and Carcharhinus brevipinna (Müller & Henle, 1839)) were recognized as by‐catch in this fishery. Elasmobranch by‐catch was dominated by sharks (90.3%), smoothhound sharks Mustelus sp. being by far the most important (88.9%) and reflecting their abundance in the area; 58% of the sets caught at least one specimen, with 4.8 ± 1.3 caught per set. Captures were composed essentially of neonate and juvenile sharks, while the batoids were dominated by mature individuals. This study shows that shrimp trammel nets represent a considerable source of mortality for early life stages of elasmobranch species in the Gulf of Gabès. Additionally, there was a high density of neonates and small juvenile M. mustelus in the Sfax zone, suggesting that these nearshore waters are a nursery grounds for smoothhound sharks. Further research should focus on the incidents of by‐catch and evaluate the potential solutions to allow trammel net fisheries to coexist alongside the elasmobranch species.
      PubDate: 2016-04-02T02:41:21.642009-05:
      DOI: 10.1111/jai.13061
  • Length–weight relationship of five commercially important freshwater
           fish species in the Kashmir Valley, India
    • Authors: A. Bashir; N. K. Sharma, B. S. Bisht, R. Singh, J. I. Mir, M. S. Akhtar
      Abstract: Length–weight relationships were analysed for five commercially important freshwater fishes, namely, Bangana diplostoma (Heckel, 1838), Schizopyge niger (Heckel, 1838), Schizothorax curvifrons (Heckel, 1838), Schizothorax plagiostomus (Heckel, 1838) and Glyptosternon reticulatum (McClelland, 1842) from different water bodies in the Kashmir Valley, India. A total of 610 samples were collected between October 2013 and May 2015 using various indigenous cast nets. Of these five species, Schizopyge niger has a new maximum length record for the FishBase LWR database.
      PubDate: 2016-04-02T02:40:58.438831-05:
      DOI: 10.1111/jai.13065
  • Length–weight relationships of two fish species from the Yangtze
           River, China
    • Authors: L. Y. Wu; J. R. Lan, C. Cheng, Q. S. Tan
      Abstract: Fish samples for the study were collected from the Yangtze River in China. Length–weight relationships were determined for two endemic fish species (Leiocassis longirostris Günther, 1864 and Schistura fasciolata Nichols & Pope, 1927) for the first time.
      PubDate: 2016-04-02T02:40:38.664162-05:
      DOI: 10.1111/jai.13068
  • The effects of different cycles of starvation and re‐feeding on
           growth and body composition in rainbow trout (Oncorhynchus mykiss,
           Walbaum, 1792)
    • Abstract: This study was designed to investigate the effects of starvation and re‐feeding cycles on the growth performance and body chemical composition of Oncorhynchus mykiss juveniles. A total of 360 juveniles with initial mean weights (IW) of 8.46 ± 0.07 g (n = 360) were stocked into 400‐L tanks in triplicate for each group, with 30 juveniles per tanks. The control group received regular feed, as is the common practice. The three other groups were periodically starved: 1 day starvation followed by 6 days re‐feeding (S1), 2 days starvation followed by 5 days re‐feeding (S2) and 3 days starvation followed by 4 days re‐feeding (S3). The experiment lasted for 10 weeks, over the course of which the water flow rate was 4 L min−1 and the water quality parameters determined as: temperature 14.4 ± 1.1°C, oxygen 8.2 ± 0.4 mg L−1 and pH 7.5 ± 0.2. At the end of the study, S1 had the best growth performance (final weight, specific growth rate, average daily growth) of all test groups (P 
      PubDate: 2016-04-01T05:02:54.597565-05:
      DOI: 10.1111/jai.13045
  • Effects of β‐xylanase and 6‐phytase on digestibility,
           trace mineral utilisation and growth in juvenile red tilapia, Oreochromis
           niloticus (Linnaeus, 1758) x O. mossambicus (Peters, 1852), fed declining
           fishmeal diets
    • Authors: J. L. Wallace; F. J. Murray, D. C. Little
      Abstract: In response to the global sustainability drive to lower fishmeal (FM) inclusion in aquatic feeds, exogenous enzymes can improve nutrient digestibility in monogastric plant‐based diets. A 80‐day experiment was conducted to evaluate the combined effects of xylanase and phytase on digestibility, trace mineral utilisation and growth in juvenile red tilapia, Oreochromis niloticus x O. mossambicus, (48.8 g ± 13.9; μ ± STD) fed declining FM diets. Basal diets were formulated to contain 0, 3 and 5% FM with and without xylanase (0.385 g kg−1) and phytase (0.075 g kg−1), forming six treatments. Each treatment was randomly assigned to four replicates, 20 fish tank−1; mean water temperature 28.98 ± 0.73°C. Although the size of the effects was modest, growth performances (feed intake, FCR, growth rate) decreased with lower FM levels (P 
      PubDate: 2016-04-01T05:02:08.776559-05:
      DOI: 10.1111/jai.13055
  • Occurrence and prevalence of Philometra filiformis (Stossich, 1896) on
           Pagellus erythrinus (Linnaeus, 1758) in the Southern Tyrrhenian Sea
    • Authors: F. Marino; B. Busalacchi, T. Bottari, P. Rinelli, G. Gaglio
      Abstract: In this paper the host‐parasite relationship between Philometra filiformis and the common pandora Pagellus erythrinus from the Southern Tyrrhenian Sea were studied. A total of 305 (257 females and 48 males) specimens of common pandora were sampled from June 2009 to April 2011 from commercial landings as well as from bottom trawl surveys. A total of 39 P. erythrinus were parasitized (mean prevalence, P = 12.80%; mean abundance, A = 0.19), with the parasite occurring throughout the study area and with wide fluctuations in prevalence values ranging from 0 to 28.60%. Prevalence increased with age of the host, with most parasites (27.78%) found in the older common pandora whereby P. filiformis parasitized only the females. A significant correlation between prevalence, abundance and maturity stages of the fish was evident, with most parasites found in mature/spawning specimens. The varying degrees of gonadic damage caused by this nematode reveal a serious threat to the reproductive success of P. erythrinus.
      PubDate: 2016-03-28T02:21:25.900212-05:
      DOI: 10.1111/jai.13069
  • Age, growth, mortality and population structure of silver croaker Pennahia
           argentata (Houttuyn, 1782) and red bigeye Priacanthus macracanthus Cuvier,
           1829 in the north‐central Taiwan Strait
    • Abstract: The main objectives of this study were to estimate the age, growth, mortality and population structure of silver croaker Pennahia argentata (Houttuyn, 1782) and red bigeye Priacanthus macracanthus Cuvier, 1829 and to explore the factors causing the fluctuation of the fish populations and the ecological parameters. The two species were randomly sampled, with 80~120 individuals of each species captured every month from a single trawler fishery (mesh size 250 mm), March to November 2006, in the north‐central Taiwan Strait. A total of 994 speciments of silver croaker and 851 speciments of red bigeye were collected. Compared with previous studies, the mean length and weight, mean age, the minimum size at first sexual maturity, the asymptotic length (L∞) and weight (W∞) of the two species has decreased in recent decades, indicating that the populations were younger, smaller and earlier in sexual maturity. Meanwhile, the growth coefficient (K) increased, the mortality coefficients (Z, F, M) were higher, and the exploitation rate (E) indicated overfishing. Fishing is a key driving force that can cause abundant fluctuations and changes in ecological characteristics in these two demersal fishes in this area. Accordingly, the traditional fishery management approach, such as the reduction in fishing effort, coupled with the Ecosystem‐based Fishery Management (EBFM) should be implemented to achieve sustainable demersal fisheries.
      PubDate: 2016-03-28T02:21:06.721581-05:
      DOI: 10.1111/jai.13053
  • Initial study on catch, species composition and reproductive biology of
           fishes off the south‐west coast of Sri Lanka, targeted by ring nets
           while utilizing natural floating objects
    • Authors: K. B. E. Chathutika; D. C. T. Dissanayake
      Abstract: This study evaluates the catch rates, species composition and reproductive biology of flotsam‐associated fishes targeted by ring nets off the southwest coast of Sri Lanka. Catch and number of multiday boats operating with ring nets were collected at Beruwala fishery harbor on the southwest coast of Sri Lanka, March to October 2013, by making fortnightly field visits. Fish samples were collected randomly from the unloaded ring net catches to analyze reproductive biology. Twelve fish species belonging to four major families: Scombridae, Carangidae, Coryphaenidae and Balistidae were identified in the ring net catches and five species, Decapterus russelli, Katsuwonus pelamis, Thunnus albacares, Elagatis bipinnulata and Canthidermis maculatus were predominant. An average of 25 ± 12% multiday boats landing at the Beruwala fishery harbor operated with ring nets each month, with an average monthly catch rate fluctuating from 730 ± 101 to 3924 ± 1094 kg per boat per trip. Catch rates of tunas and carangids were significantly higher than the other fish groups (anova; d.f. = 3, P 
      PubDate: 2016-03-28T02:20:43.500923-05:
      DOI: 10.1111/jai.13054
  • Catch composition and some other aspects of bottom trawl fishery in
           Sığacık Bay, central Aegean Sea, eastern Mediterranean
    • Abstract: The objective of the study was to determine the features of the bottom trawl fishery such as catch composition, catch per unit effort (CPUE) and the discard ratios in the Sığacık Bay fishery, central Aegean Sea. A commercial trawler conducted trawl operations from October 2008 to April 2009, with a total of 18 trawls in autumn, winter and spring (six hauls per season). In total, 84 species were identified, 47 of which belonged to the bony fishes, while other groups contributed nine (cartilaginous fish), 10 (cephalopods), 13 (crustaceans), four (echinoderms) and one (porifera) species. Some 49 species were discarded regardless of length. Total catch was 3693.2 kg of which 66.8% was marketed. Among the commercial species, rose shrimp (Parapenaeus longirostris) dominated the catch composition with 998 kg. However, discards accounted for 33.2% of the total catch biomass, with the majority represented by Lampanyctus crocodilus with 97 kg total weight. The study area is considered one of the most efficient trawling zones in the Aegean Sea and thus requires long‐term monitoring in terms of fish stocks and trawl discards for the preparation of well‐informed decisions for a sustainable fishery.
      PubDate: 2016-03-24T02:42:24.381832-05:
      DOI: 10.1111/jai.13042
  • Microbiological changes in whiting (Merlangius merlangus Linneaus, 1758)
           fillets during short‐term cold storage and a traditional
           ‘pastrami‐like’ treatment
    • Abstract: The objective of this study was to test the effects of salt‐dried whiting (Merlangius merlangus) fillet storage when treated with a special paste and stored covered. For this purpose whiting fillets were salt‐dried at 4–6°C for 15 days. A subsequent test series involved a paste mixture prepared from ground fenugreek, cumin seeds, black pepper, red pepper powder and garlic. The fillets were coated with this paste and air‐dried (15–20°C) for 5 days. All microbiological changes during this drying period were noted. The aerobic mesophilic bacterial counts decreased significantly (P 
      PubDate: 2016-03-24T02:41:58.441136-05:
      DOI: 10.1111/jai.13043
  • Hatchery performance in a major rainbow trout (Oncorhynchus mykiss,
           Walbaum, 1792) seed production area of Turkey
    • Abstract: This study aims to determine the production characteristics and performance of rainbow trout hatcheries in Seydikemer, Muğla, Turkey where over half of the total production of eyed eggs and juveniles take place. For this purpose six hatcheries with different production scales were selected and coded A, B, C, D, E and F (with capacities of 60, 15, 5.6, 4, 0.95 and 0.6 million juveniles per year, respectively). From each hatchery, 20 females were selected and their egg qualities (egg number, diameter and weight, fertilization, eyed egg, hatching, swim‐up fry and survival rates at 120 days after hatching) and juvenile growth rates were monitored until the 120th day after hatching under each farms own conditions. The sperm characteristics of 10 broodstock males from each hatchery were also examined. Although fertilization and eyed egg rates were similar among the farms, hatching, swim‐up fry, and survival rates at the 120th day after hatching differed significantly. The average eyed egg, hatching and survival rates (calculated from selected 20 females) were 72, 55, and 32%, respectively. While sperm characteristics except duration of motility (s), were significantly different among the farms, average sperm concentration (per ml), motility (%) and duration of motility (s) in the region were 11.8 × 109, 55.3, and 56.4, respectively. The best hatchery performance was observed in Farm A because of better records and management applications than in the other farms.
      PubDate: 2016-03-24T02:41:37.161486-05:
      DOI: 10.1111/jai.13063
  • Length–weight relationships of Dermogenys pusilla Kuhl & van
           Hasselt, 1823 (Zenarchopteridae) and Labeo bata (Hamilton, 1822)
           (Cyprinidae) from the Ganges River (NW Bangladesh)
    • Authors: M. Y. Hossain; M. A. Hossen, M. N. U. Pramanik, K. Yahya, A. H. Bahkali, A. M. Elgorban
      Abstract: Length‐weight relationships (LWRs) were determined for Dermogenys pusilla (n = 75) and Labeo bata (n = 304) from the Ganges River, northwestern Bangladesh, collected between July 2013 and June 2014, using traditional fishing gear (e.g. cast net, square lift net and gill net). Total length (TL) was measured to 0.1 cm and whole body weight (BW) was taken to the nearest 0.1 g for each individual. The TL varied from 6.60 to 16.10 cm for D. pusilla and 7.90–25.20 cm for L. bata. The BW ranged from 1.20 to 10.90 g for D. pusilla and 4.70–167.30 g for L. bata. All LWRs were highly significant (P 
      PubDate: 2016-03-24T02:41:15.247115-05:
      DOI: 10.1111/jai.13070
  • Twenty‐four novel microsatellites for the endangered Chinese
           sturgeon (Acipenser sinensis Gray, 1835)
    • Authors: M.M. Xin; S.H. Zhang, D.Q. Wang, C.J. Li, H.M. Yue, Q.W. Wei
      Abstract: The Chinese sturgeon (Acipenser sinensis) is an endemic and critically endangered species in China. In this study, a total of 24 polymorphic microsatellite loci were isolated and characterized using Illumina sequencing for A. sinensis. The number of alleles (Na) per locus ranged from 2 to 6 (mean 4.04), mean expected heterozygosities (He), Shannon‐Wiener Diversity Indices (SW) and evenness (E) per locus ranged from 0.235 to 0.786 (mean 0.62), from 0.396 to 1.608 (mean 1.13), and from 0.060 to 0.213 (mean 0.13), respectively. Exact tests revealed that nine loci showed significant (P 
      PubDate: 2016-03-24T02:40:56.460214-05:
      DOI: 10.1111/jai.13071
  • Feeding ecology of a bagrid catfish, Mystus tengara (Hamilton, 1822) in
           the Tanore wetland of Rajshahi, Northwestern Bangladesh
    • Authors: N. R. Mitu; M. M. Alam
      Abstract: The feeding ecology of Mystus tengara (Hamilton, 1822) was studied in the Tanore wetland of Rajshahi, northwestern Bangladesh from January 2011 to December 2011. In fish purchased from the local market, a total of 14 713 food items were identified in the stomach contents of 3191 M. tengara specimens, an omnivorous catfish with a diversified feeding strategy including cladocerans, copepods, rotifers, gastropods, green algae, diatoms, insects, ‘small fish remains, fish fry and eggs’ and ‘other’ food items. Insects were the most dominant group of prey throughout the year. Seasonal changes in diet composition were due to the availability of prey items and fish size. Ontogenetic changes were noted between the juveniles and adults. The gut contents of M. tengara showed that the food items were from different layers of the water bodies.
      PubDate: 2016-03-23T01:52:13.831015-05:
      DOI: 10.1111/jai.13049
  • Morphology and parentage association of shortened upper jaw deformity in
           hatchery‐produced Japanese flounder, Paralichthys olivaceus
           (Temminck & Schlegel, 1846)
    • Authors: E. Sawayama; M. Takagi
      PubDate: 2016-03-23T01:51:50.188771-05:
      DOI: 10.1111/jai.13056
  • A new approach to quantifying setline angler effort and catch
    • Authors: B. L. Eder; M. A. Pegg, G. E. Mestl
      PubDate: 2016-03-23T01:51:27.587968-05:
      DOI: 10.1111/jai.13057
  • Length–weight relationships and length–length relationships of
           13 fish species in Rongcheng Bay, China
    • Authors: L. J. Wang; Z. H. Wu, M. M. Nie, M. X. Liu, W. Liu, F. You
      Abstract: The present study provides the length–weight relationships (LWRs) of 13 fish species belonging to eight families that were collected in Rongcheng Bay, March 2009 to December 2014. The r2 values ranged from 0.953 to 0.994 and values of b varied from 2.542 to 3.454. The study also describes the total length – standard length relationships (LLRs) for these fish species. This is the first record of length–weight and length–length relationships for the fish species in this area.
      PubDate: 2016-03-23T01:51:07.475402-05:
      DOI: 10.1111/jai.13058
  • Critical thermal maxima of juvenile alligator gar (Atractosteus spatula,
           Lacépède, 1803) from three Mississippi‐drainage
           populations acclimated to three temperatures
    • Authors: A. V. Fernando; S. E. Lochmann, A. H. Haukenes
      Abstract: Local adaptation may cause thermal tolerance to vary between nearby but distinct populations of a species. During the summer of 2013, alligator gar Atractosteus spatula spawned from broodstock collected from three populations within the Mississippi River drainage separated by a 5° latitudinal gradient were acclimated to three temperatures (25, 30, and 35°C). Ten fish from each population were acclimated at each temperature. CTMax was determined at each temperature for each population, using five fish for each population‐acclimation temperature pairing. CTMax for each population‐acclimation temperature pairing was compared using two‐factor anova. CTMax increased significantly with acclimation temperature (F2,40 = 600.5, P 
      PubDate: 2016-03-22T00:40:43.497427-05:
      DOI: 10.1111/jai.13047
  • Population genetics of Atlantic bluefin tuna, Thunnus thynnus (Linnaeus,
           1758), in the Mediterranean: implications for its conservation
    • Abstract: The movement of Atlantic bluefin tuna (ABFT) across international boundaries necessitates traceability strategies that would provide more accurate information needed for stock assessment. The Mediterranean Sea is one of the main contributors to ABFT reproduction and global population genetic diversity. In the present study this genetic variability was investigated using 193 samples of adult bluefin tuna from Spain, Turkey and Malta – a longitudinal distance of 3400 km. Analysed were 13 microsatellite loci (eight of which were newly‐tested) as genetic markers for the population study. Allele richness measured per locus and sampling location varied from 1.89 to 8.88, taking into account rarefaction. ABFT private alleles were detected in each of the three sampling sites. No significant spatial genetic divergence was found between pairs at the studied locations (FST values 0.05). Bayesian clustering analysis corroborated a single and panmictic ABFT population in the Mediterranean Sea. Statistical power analyses indicated a high probability of detecting genetic differentiation and population structure with the sample size and microsatellites used, even at an FST value of 0.005. From the results it may be postulated that migrating ABFT during the spawning season are allowing gene flow within the Mediterranean Sea. The complex interplay of movements, including plasticity in the selection of spawning sites with increasing age and environmental conditions, require multiple and new fisheries monitoring and management techniques in order to target the ABFT long‐term conservation effectively.
      PubDate: 2016-03-09T12:53:07.488433-05:
      DOI: 10.1111/jai.13035
  • Types and extent of fishing gear losses and their causes in the artisanal
           fisheries of Istanbul, Turkey
    • Abstract: Fishing gear losses remain a serious problem for marine life; these losses are also the source of an invisible fishing mortality. This study is an analysis of this problem within the artisanal fisheries of Istanbul. From September 2009 through May 2010, face‐to‐face interviews were conducted in 27 fishing ports for a total of 282 questionnaires. Estimates of lost fishing gear were 229.48 km of set nets, 2700 m of longlines, and 14 fish traps. Turbot nets had the highest loss ratio (54.73%), followed by bonito nets (16%), red mullet nets (7.36%), and encircling trammel nets (4.83%). The four major causes of net loss were: conflicts with other gear types (trawl and purse seine), conflicts with cargo vessels, bottom structure hindrances, and bad weather conditions. Bottom structures were the single factor in net losses for longlines and fish traps. The study observed that large quantities of nets had been lost, and that each net type showed a positive relationship between the number of nets used and the number of nets lost. Separate zones for artisanal and industrial fisheries are recommended as a realistic approach to reduce the losses of fishing gear and the impact of ‘ghost fishing’.
      PubDate: 2016-03-09T12:51:56.342024-05:
      DOI: 10.1111/jai.13046
  • A complete protocol for the preparation of chondrichthyan skeletal
    • Abstract: Summary The skeleton of cartilaginous fishes is notoriously difficult to prepare, and the literature dealing with the subject is extremely rare compared to other vertebrate groups. As a result, chondrichthyan skeletal specimens held in museums and other scientific institutions are often limited to isolated sets of jaws, taxidermised specimens and small individuals preserved whole in fluid. However, skeletal characters are heavily relied upon in several taxonomical and phylogenetical analyses, highlighting the importance of properly prepared skeletal specimens in research collections. The relevant literature is briefly reviewed herein, with a proposal for a new and simple protocol involving limited and easily obtainable chemicals, for the quick preparation of clean and durable chondrichthyan skeletal specimens. Various features of the chondrichthyan skeleton are also discussed, along with the microstructural effects of the chemicals involved in the protocol.
      PubDate: 2016-03-09T12:51:35.002088-05:
      DOI: 10.1111/jai.13050
  • Chemical and sensory quality changes in fish balls prepared from Alburnus
           mossulensis Heckel, 1843 during frozen storage
    • Authors: M. Duman; B. Peksezer
      Abstract: The effects of frozen storage on fish balls derived from Alburnus mossulensis at −18 ± 2°C were studied. Several chemical parameters were determined [pH, total volatile basic nitrogen (TVB‐N), thiobarbituric acid (TBA), free fatty acids (FFA)]. Further, microbiological effects were analysed during storage [Total mesophilic aerobic bacteria (TMAB), total psychrophilic bacteria (TPB), coliform and yeast‐mould count]. Finally, sensory quality tests were performed with fresh and stored balls. Fish balls were composed of 64% fish meat and 36% other ingredients, including: 18% boiled rice, 11.4% onions, 1.8% parsley, 3.8% fat, 0.7% salt and 0.26% black pepper. This basic mixture was processed to form small (25 g) balls and a pre‐cooking process was applied. Test balls were divided into two groups: vacuum packed (A1) and a control group without vacuum (A2). Test packages were stored for 6 months, resulting in increased pH, TVB‐N, TBA and FFA, while the values for sensory properties declined. However, these changes did not drop below the limit of acceptance. Total mesophilic aerobic bacteria and total psychrophilic bacteria were significantly reduced in both A1 and A2 groups.
      PubDate: 2016-03-09T12:51:07.157206-05:
      DOI: 10.1111/jai.13039
  • Effects of different baits on monofilament gillnet effectiveness in a
           freshwater reservoir fishery (Keban Dam Lake, Turkey)
    • Authors: M. Dartay; E. Duman
      Abstract: Aim of this study was to test the gillnet efficiency in Keban Dam Lake using different baits as attractants. Gillnets of 50 m length (stretched mesh sizes: 40, 50, 55 mm) were employed between January 2009 and December 2010 in the study. Two types of grain (corn and wheat) as well as processed plant material (beet cake) and animal livers were tested as bait to attract both carnivorous and omnivorous species. The catching efficiency of baited and non‐baited gill nets was compared. Overall, a total of 358 fish from eight different species were caught in baited gillnets, whereas only 189 fish were caught with non‐baited gillnets. Most fish (55.90%) were caught with corn, wheat or beet cake as bait; the fewest (59 fish, 9.96%) were captured with animal liver. About 34.1% were caught with non‐baited gillnets. By species, Capoeta trutta (Cyprinidae) was most frequently captured. In conclusion, the gillnets bated with corn, wheat, and beet cake yielded higher catches (P 
      PubDate: 2016-03-09T12:50:47.777952-05:
      DOI: 10.1111/jai.13038
  • Effects of clove oil, essential oil of Lippia alba and 2‐phe
           anaesthesia on juvenile meagre, Argyrosomus regius (Asso, 1801)
    • Abstract: The objectives of this experiment were to (i) determine the efficacy of essential oils of clove (CO) and Lippia alba (EOLA) to induce deep anaesthesia in juvenile specimens (49.0 ± 6.2 g body mass, 16.6 ± 0.8 cm; n = 8 per treatment) of meagre (Argyrosomus regius); and (ii) study the feasibility of these substances, together with 2‐phenoxyethanol (2‐PHE), as potential sedatives [low concentration: (i) EOLA: 12 mg L−1; (ii) CO: 1 mg L−1; and (iii) 2‐PHE: 33 mg·L −1; n = 8 per treatment] for live fish transport of this species. All test were performed at a constant temperature (18°C). Thus, the main primary stress indicator (plasma cortisol) and secondary factors (plasma metabolites) were evaluated. In addition, growth hormone (GH) mRNA expression was also evaluated in the pituitary gland. The results indicated that EOLA is considered to be effective for deep anaesthesia when the concentration is close to 160 mg L−1, while CO produces the same effect when lower concentrations are added (40–50 mg L−1). Regarding sedative concentrations, a significant ~3‐fold increase in plasma cortisol levels was detected in the EOLA group when compared to control specimens. In addition, glucose levels were not reduced and significantly increased (~1.6‐fold) for 2‐PHE in relation to the control fish. None of the anaesthetics promoted a significant difference for GH expression with respect to the control group, but a significant ~2‐fold increase for 2‐PHE treatment with respect to the EOLA exposition was found in this gene expression. Results show that none of the anaesthetics analysed, at least in the ranges of concentrations used in this study (EOLA 12 mg L−1, CO 1 mg L−1, 2‐PHE 33 mg L−1), are recommended for live fish transport, as shown by the absence of inhibition on the stress parameters assessed.
      PubDate: 2016-03-09T12:50:27.203819-05:
      DOI: 10.1111/jai.13048
  • Glutamine supplementation improves frozen‐thawed beluga sturgeon,
           Huso huso (Linnaeus, 1758) semen
    • Authors: M. S. Aramli; R. M. Nazari
      PubDate: 2016-03-09T12:50:20.911601-05:
      DOI: 10.1111/jai.13052
  • A comparison of fatty acid, cholesterol and vitamin composition in sea
           bass [Dicentrarchus labrax (Linnaeus, 1758)] and sea bream [Sparus aurata
           (Linnaeus, 1758)] from three cage farm areas: Antalya and Muğla
           (Turkey) and İskele (Northern Cyprus)
    • Abstract: In the present study the fatty acids, cholesterol and vitamin composition in farmed sea bass (8 fish per species per farm; weight range: 389.6–395.8 g, total length range: 297–316 mm) and sea bream (8 fish per species per farm; weight range: 386.8–391.7 g, total length range: 263–268 mm) from three cage farms (İskele in northern Cyprus, Antalya and Muğla in Turkey) were compared during the harvesting period in June–July 2011. The results showed that the muscles of D. labrax and S. aurata farmed fish were rich in n‐3 fatty acids, but with important differences. For example, the muscles of sea bass farmed in İskele were rich in docosahexaenoic acid (DHA) and n‐3 polyunsaturated fatty acids (PUFA). Palmitic acid (C16:0) was the primary saturated fatty acid, and oleic acid (C18:1 n‐9) the primary monounsaturated fatty acid in the muscle and liver samples of the cage‐farmed sea bass and sea bream. There were no significant differences in the cholesterol content in the muscles of sea bream farmed in İskele, Antalya or Muğla. In conclusion, the n‐3/n‐6 ratio in the muscle of farmed S. aurata and D. labrax is within the recommended limits for a healthy human diet, being very suitable for human nutrition.
      PubDate: 2016-03-08T00:43:33.994187-05:
      DOI: 10.1111/jai.13036
  • Tocopherol, heavy metals (Cd, Pb, Cu, Fe, Mn, Zn), and fatty acid contents
           of thornback ray (Raja clavata Linnaeus, 1758) liver oil in relation to
           gender and origin in the Mediterranean and Black seas)
    • Authors: A. Ozyilmaz
      Abstract: The study objective was to investigate the profiles of tocopherol, heavy metals, and fatty acids in the liver oil of female and male thornback ray Raja clavata from the Black and Mediterranean seas. The fish liver oil analyses were carried out by using a spectrophotometer (Hitachi U‐1900), ICP‐AES (Inductively Coupled Plasma‐Atomic Emission Spectrometry, and a GC‐MS (gas chromatography‐mass spectrometry), respectively. Results showed that the tocopherol content differed between the sample origin and gender (range of 42.3–134.5 mg kg−1). Pb, Cd, and Mn contents in livers of all R. clavata were lower than for Cu, Fe, and Zn. Levels of 7.6 and 8.9% in the eicosapentaenoic acid (EPA) were found to be higher in Black Sea thornback rays than in Mediterranean specimens (4.5 and 5.8%) (P 
      PubDate: 2016-03-08T00:43:14.983741-05:
      DOI: 10.1111/jai.13041
  • Growth and life history traits of Aegean chub, Squalius fellowesii
           (Günther, 1868) in streams in Muğla Province, Aegean coast,
    • Abstract: To aid in species' conservation, the aim of this study was to provide initial findings on age, growth and reproduction of an endemic species, Aegean chub Squalius fellowesii (Günther, 1868) populations from streams in the Aegean region of Muğla Province, Turkey. The species is relatively short‐lived (maximum 6 years), attaining a size of about 200 mm total length with a rapid growth to first maturity (≈60 mm TL), and relatively little growth thereafter. The male:female ratio was 1.0 : 0.6, males significantly outnumbering females in the majority of the streams. General condition values of individual fish varied between 2.9 and 3.4. Sexual maturity was usually achieved later and at larger sizes in females than in males. Sexual maturation in most populations was at the age of 2 years in females and 1 year in males. The species spawns between early April and late May. Mean absolute and relative fecundity were about 4440 eggs and 57 eggs·g−1, respectively. Mean egg diameter was 1.00 ± 0.03 mm, ranging from 0.70 to 1.20 mm. Suggestions for the conservation of Aegean chub are discussed.
      PubDate: 2016-03-08T00:42:53.866632-05:
      DOI: 10.1111/jai.13040
  • Ovarian development of albacore (Thunnus alalunga Bonnaterre, 1788) in the
           Eastern Mediterranean Sea: a histological characterization
    • Authors: F. S. Karakulak; T. Akayli, I. K. Oray, R. E. Yardimci
      Abstract: In order to investigate the unclear status of albacore (Thunnus alalunga) reproduction in the Eastern Mediterranean Sea, the stages of oogenesis from 129 females (64.0 and 83.5 cm fork length) were collected and analysed between April and August for the years 2002 and 2008. Fish were captured by commercial vessel gillnets. Based on histological examination of the ovaries, a total of seven ovarian developmental stages were identified: (i) perinucleolar, (ii) phase of early lipid enrichment, (iii) early vitellogenesis, (iv) late vitellogenesis, (v) migratory nucleus, (vi) pre‐hydrated and (vii) hydrated oocyte phase. Data on the monthly gonadosomatic index and the results of the histological analyses indicate that in the region the female T. alalunga mature in May. The spawning stage is predominant in June, reaching a maximum in July. The evidence reported here indicates that size at first sexual maturity for females was lower for the Mediterranean albacore stock than for the Pacific and Atlantic stocks.
      PubDate: 2016-03-06T23:22:25.492636-05:
      DOI: 10.1111/jai.13034
  • Effect of absorbent pads containing black seed or rosemary oils on the
           shelf life of sardine [Sardina pilchardus (Walbaum, 1792)] fillets
    • Authors: B. Kilinc; S. Altas
      Abstract: The effects of essential oils on the shelf life of sardine (Sardina pilchardus) fillets in cold storage were assessed at 4–6°C. The oils were sprayed on the fillets as well as with the addition of absorbent food pads that also contained essential oils. Sardine fillets were divided into eight test groups. Group A: fillets stored without treatment (control group); Group B: fillets packaged with food pads (without the addition of essential oils); Group C: Rosemary oil sprayed onto food pads (10 ml of 1.5% RO); Group D: Black seed oil sprayed onto the food pads (10 ml of 1.5%BSO); Group E: Rosemary oil sprayed onto both sides of the sardine fillets (10 ml of 1.5%RO); Group F: Black seed oil sprayed onto both sides of sardine fillets (10 ml of 1.5% BO); Group G: Rosemary oil sprayed onto both sides of the sardine fillets (10 ml of 1.5% RO) and on the food pads (+10 ml of 1.5% RO); and Group H: Black seed oil sprayed onto the sardine fillets and the food pads (10 ml of 1.5% BSO onto both sides of fillets +10 ml of 1.5% BSO onto food pads). Tests were carried out at days 0, 3, 5 and 7. When compared to the control group, food pads containing rosemary and black seed oils extended the shelf life of sardine fillets by approximately 2 days at 4–6°C. Group A and B exceeded the limit of consumption on day 3, and Group C and D exceeded this microbiological limit after day 5. RO and BSO both exhibited the same antimicrobial effects on the shelf life of sardine fillets. Groups E, F, G and H prolonged the microbiological threshold by 7 days compared to the control. However, Group G and H had 1–1.5 log lower TVC load than Group E and F. Food pads containing antimicrobial essential oils may be used to extend the shelf life of fresh fish, however, further investigations are needed to determine safety standards.
      PubDate: 2016-03-06T23:20:11.18852-05:0
      DOI: 10.1111/jai.13044
  • Morphological and morphometric evaluation of silver barb, Barbodes
           gonionotus (Bleeker, 1849) sperm supplemented with antibiotics
    • Authors: T. Boonthai; W. Khaopong, J. Sangsong, S. Nimrat, V. Vuthiphandchai
      Abstract: Aim of this study was to evaluate sperm morphology of silver barb, Barbodes gonionotus, sperm and describe the effect of antibiotics on morphological characteristics of the sperm using an ASMA plug‐in. The experiment was done at the room temperature (25°C) and divided into four treatments in three replicates: (i) freshly collected semen, (ii) extended semen (control), (iii) extended semen supplemented with 0.5% penicillin‐streptomycin (PS), and (iv) extended semen supplemented with 0.5% penicillin‐gentamicin (PG). Silver barb sperm comprised three main compartments: a circular head with no acrosome, a midpiece, and a single flagellum. Addition of 0.5% PS had no detrimental effects on sperm morphometry, except flagellum width. Administration of 0.5% PG affected sperm morphology in two distinct ways: (i) intact sperm (76.92 ± 5.84% of total sperm) except for flagellum width, and (ii) severe morphological damage (23.08 ± 2.67%).
      PubDate: 2016-02-24T05:15:12.99391-05:0
      DOI: 10.1111/jai.12960
  • Length–weight relationships of six fish species from the Lancang
           River, China
    • Authors: H. Y. Sun; J. L. Yao, Y. Cheng, Y. F. Chen
      Abstract: Length–weight relationships of six fish species are presented. Fish samples were collected from the Lancang River, in the Chinese section of the Upper Mekong River, in southwestern China. This study reports the first length–weight relationship for one of these species.
      PubDate: 2016-02-22T05:14:47.46558-05:0
      DOI: 10.1111/jai.13007
  • Feeding habits of the Pacific bearded brotula Brotula clarkae Hubbs, 1944
           (Ophidiidae) along the Pacific coast of Costa Rica, Central America
    • Abstract: The present study analyzed the diet composition, ontogenetic shifts and dietary overlap of Brotula clarkae in relation to stage of maturity and sex. Samples were collected from the trawling fishery along the Pacific coast of Costa Rica (2011–2012) at depths ranging from 41.4 to 168.3 m; however, over 80% of the sampled fish were obtained at depths between 50 and 75 m. Size ranged from 14.4 to 98.4 cm total length. Of the 323 analyzed stomachs, 44.3% were from males, 86% were from immature individuals, and 49.8% had at least one prey item. According to the prey‐specific index of relative importance (PSIRI), decapod shrimps were the most important prey (57.6% PSIRIi) followed by teleosts (28.2% PSIRIi), stomatopods (10.8% PSIRIi), and crabs (3.3% PSIRIi). Male and female B. clarkae exhibited a high dietary overlap (CH = 0.94). Immature B. clarkae consumed primarily shrimps and crabs (71.5% of stomachs from immature specimens contained shrimps, which accounted for over 66.0% PSIRIi); mature individuals consumed a large proportion of teleosts and stomatopods, which together contributed to over 91.0% PSIRIi. Both immature and mature B. clarkae overlapped spatially with the commercial trawling fishery grounds along the Pacific coast of Costa Rica. However, juveniles feed predominantly on shrimps, suggesting that immature B. clarkae may be subjected to high fishing pressure as by‐catch, making them particularly vulnerable to overexploitation.
      PubDate: 2016-02-11T03:17:54.929453-05:
      DOI: 10.1111/jai.13029
  • Conservation genetics assessment and phylogenetic relationships of
           critically endangered Hucho bleekeri in China
    • Abstract: Hucho bleekeri is a critically endangered salmonid fish found in the Yangtze River drainage in China. In this study, the genetic diversity of a small population (n = 43) was first assessed with partial mitochondrial DNA sequences (D‐loop region and a cytochrome b gene [CYTB] gene fragment) and 15 microsatellite markers. Low levels of nucleotide diversity (Pi) were demonstrated in the H. bleekeri population based on the two mitochondrial DNA markers. The number of haplotypes (h) and the haplotype diversity (Hd) in the D‐loop region (12 haplotypes and Hd = 0.8208) were higher than in the CYTB gene fragment (three haplotypes and Hd = 0.0941). The number of microsatellite alleles (Na) ranged from 2 to 13 in these individuals. The mean observed heterozygosity (Ho) and the expected heterozygosity (He) were 0.59719 and 0.44735, respectively. Analysis of molecular variance showed that the degree of differentiation in the population was low (FST = 0.04041) and the coefficient of inbreeding (FIS) was negative, indicating no obvious evidence of inbreeding in this population. A demographic assessment suggested that this species expanded a long time ago, but has suffered great losses in recent years. A molecular phylogenetic analysis clearly indicated that H. bleekeri is not introgressed by Brachymystax lenok tsinlingensis. The baseline population genetic information supplied by this study will be vital in monitoring this highly threatened species.
      PubDate: 2016-02-09T05:55:44.026241-05:
      DOI: 10.1111/jai.13018
  • Endoscopy application in broodstock management of Arapaima gigas (Schinz,
    • PubDate: 2016-02-06T01:09:06.37733-05:0
      DOI: 10.1111/jai.12988
  • Reproductive strategy of the invasive sharpbelly, Hemiculter leucisculus
           (Basilewsky 1855), in Erhai Lake, China
    • Abstract: This study describes the reproductive strategy of the sharpbelly Hemiculter leucisculus, an invasive and dominant species in Erhai Lake in China with the goal of understanding how reproductive strategy contributes to its environmental adaptability and invasive potential. Specimens (n = 3583) were collected monthly or bimonthly from July 2009 to June 2011 (min: 114‐Feb, max: 883‐Aug), using gill nets (inner/outer mesh = 30/110 mm, stretched mesh). Gonads were removed, weighed and preserved for further (histological) analyses. Oocyte size‐frequency distribution was continuous and had advanced vitellogenic oocytes with postovulatory follicles present in spawning females, both suggesting that sharpbelly is a multiple spawner with adhesive eggs. Spawning extended from April to September, with the peak period from May to August. Standard lengths (SL, cm) at minimal observed maturity/L50/L99.9 values were 4.6/5.6/10.5 for females and 5.1/5.5/7.5 cm for males, respectively. Females were over‐represented (P 
      PubDate: 2016-02-06T01:08:46.665135-05:
      DOI: 10.1111/jai.13001
  • Length–weight relationships of four small fish species caught in the
           Heishui River, China
    • Abstract: In the present paper the length–weight relationships (LWRs) were determined for Jinshaia sinensis Sauvage & Dabry de Thiersant, 1874; Triplophysa anterodorsalis Zhu & Cao, 1989; Liobagrus marginatus Günther, 1892; and Glyptothorax sinensis Regan, 1908 caught in the Heishui River in July 2014. The specimens were caught with fishing gear including drifting gillnets (mesh size 4 cm) and shrimp cages. This paper provides new data on LWRs for FishBase.
      PubDate: 2016-02-06T01:08:18.511705-05:
      DOI: 10.1111/jai.12990
  • Longtail tuna Thunnus tonggol (Bleeker, 1851) shows genetic partitioning
           across, but not within, basins of the Indo‐Pacific based on
           mitochondrial DNA
    • Authors: D. A. Willette; M. D. Santos, D. Leadbitter
      Abstract: Genetic stock structure is atypical in tuna species, with most species demonstrating geographically‐broad, panmictic populations. Here, genetic data suggest a distinct pattern for Thunnus tonggol across the Indo‐Pacific region. The genetic variation in the coastal tuna T. tonggol sampled from across the South China Sea was examined using the highly variable mitochondrial DNA displacement loop (D‐loop) gene region. One hundred and thirty‐nine specimens were sampled from four locations in Indonesia, Vietnam and the Philippines. Phylogenetic reconstruction of genetic relationships revealed no significant ɸST statistics and hence no population structure within the South China Sea. However, subsequent analysis with sequence data from coastal northwest India infers discrete genetic stocks between the Indian Ocean and the South China Sea. Consistent with previous genetic analyses of tuna species in the Indo‐Pacific, the findings in this study infer no population structure within each basin, but rather show a significant partitioning across the wider region. Furthermore, these results have implications for the management of the commercially valuable Thunnus tonggol across national boundaries, and thus requiring collaboration among countries to ensure its sustainable use.
      PubDate: 2016-02-06T01:07:58.302879-05:
      DOI: 10.1111/jai.12991
  • Effects of elevated nitrate concentration on early thyroid morphology in
           Atlantic salmon (Salmo salar Linnaeus, 1758)
    • Authors: A. R. Freitag; L. R. Thayer, H. J. Hamlin
      Abstract: A study was conducted to determine if exposure to elevated nitrate (NO3‐)(3.76 ± 0.07 and 93.15 ± 2.79) during embryonic development influences the morphology of thyroid tissue in newly hatched Atlantic salmon, Salmo salar. Digital three‐dimensional imaging of thyroid follicles in S. salar showed that the thyroid tissue in newly hatched alevin consisted mainly of spherical follicles dispersed throughout the lower jaw in close proximity to the ventral aorta and other blood vessels. With these digital models, the number of follicles, average volume per follicle, and total luminal volume were calculated for alevin exposed to well water or 93.15 mg L−1 NO3‐N during embryogenesis. There were no statistically significant differences in any of these parameters, suggesting that the normal thyroid development of S. salar could be relatively insensitive to excess environmental NO3‐. These results are promising for the culture of Atlantic salmon embryos in recirculating aquaculture systems that naturally accumulate NO3‐ as a product of nitrification.
      PubDate: 2016-02-06T01:07:40.398513-05:
      DOI: 10.1111/jai.13012
  • Upper lethal temperature of larval pallid sturgeon Scaphirhynchus albus
           (Forbes and Richardson, )
    • Authors: I. R. Miller; K. M. Kappenman, M. J. Talbott
      PubDate: 2016-02-06T01:07:14.956215-05:
      DOI: 10.1111/jai.12994
  • Critical thermal maxima and hematology for juvenile Atlantic (Acipenser
           oxyrinchus Mitchill 1815) and shortnose (Acipenser brevirostrum Lesueur,
           1818) sturgeons
    • Authors: M. C. Spear; J. D. Kieffer
      Abstract: The critical thermal maximum (CTmax) and the associated hematological response of juvenile (~145 g, n = 8 for both species) Atlantic Acipenser oxyrinchus and shortnose Acipenser brevirostrum sturgeons acclimated to 15°C were determined using a heating rate of 8°C h−1. The critical thermal maximum averaged 30.8°C and 31.6°C for Atlantic and shortnose sturgeon, respectively, and values fell within the range noted for other sturgeon species. Oxygen‐carrying capacity (hemoglobin and hematocrit) measures were generally unaffected by thermal stress. Plasma lactate levels increased from 0.5 mm to 4 mm following temperature stress in both species. Both plasma glucose and potassium levels increased following CTmax, however, these levels were about double in the shortnose sturgeon. Lastly, plasma sodium and chloride levels were significantly depressed (by more than 10%) following thermal stress in shortnose sturgeon, whereas only chloride levels decreased in Atlantic sturgeon. Taken together, while CTmax values were similar, thermal stress resulted in different hematological profiles; these differences are consistent when compared to other stressors, and may be related to the phylogenetic position and thus could reflect the evolutionary history of these two species.
      PubDate: 2016-02-06T01:06:56.088462-05:
      DOI: 10.1111/jai.13002
  • Length‐weight relationships of five fish species from the Yalong and
           Wujiang rivers (tributaries of Yangtze River, China)
    • Authors: L. Pan; S. R. Yang, L. Wan, Z. Yang, Z. Li, Y. Qiao
      Abstract: Length‐weight relationships (LWRs) were determined for five endemic fish species from the Yalong and Wujiang rivers (tributaries of the Yangtze River, China): Folifer brevifilis (Peters, 1881), Pelteobagrus ussuriensis (Dybowski, 1872), Schistura fasciolata (Nichols & Pope, 1927), Triplophysa daqiaoensis (Ding, 1993) and Triplophysa orientalis (Herzenstein, 1888). Samples were obtained between April 2004 and July 2014 using various fishing gear (set nets, drift gill nets, fish cages, hook and electro fishing). For each specimen from each species the sample size was recorded, total length and weight were measured and the LWR determined. Further, the 95% confidence intervals of a and b, and coefficient of correlation were estimated. Prior to this study, the LWRs for these five species were unknown.
      PubDate: 2016-02-06T01:06:32.461221-05:
      DOI: 10.1111/jai.13000
  • Occurrence of the connemarra clingfish, Lepadogaster candolii Risso, 1810
           (Gobiesocidae) in the Aegean Sea (Izmir Bay, Turkey)
    • PubDate: 2016-02-06T01:06:12.383772-05:
      DOI: 10.1111/jai.13003
  • Length–weight relationships of three fish species from Wujiang
           River, China
    • Authors: Q. Wang; C. Y. Sha, Q. Jin
      Abstract: Length–weight relationships were determined for three fish species (Pseudogyrinocheilus prochilus Sauvage & Dabry de Thiersant, 1874; Folifer brevifilis Peters, 1881; and Garra imberba Garman, 1912). Samples were collected from the Wujiang River in China, from May to July in 2009–2011 using gillnets (25 × 12 m, mesh size 0.5 cm) and fish cages (0.5 × 0.5 × 10 m, mesh size 0.5 cm mesh). Length–weight relationships for these three fish species were determined for the first time.
      PubDate: 2016-02-06T01:05:51.681721-05:
      DOI: 10.1111/jai.13028
  • Length–weight relationships and basic biological information on 64
           fish species from lower sections of the Wujiang River, China
    • Authors: Z. Yang; H. Y. Tang, Y. F. Que, M. H. Xiong, D. Zhu, X. Wang, Y. Qiao
      Abstract: The present study provides the length–weight relationships (LWRs) for 64 fish species belonging to eight families from the lower sections of Wujiang River, a tributary of the upper Yangtze River in China. LWRs for eight of these species are unknown to FishBase and new maximum body weights are recorded for 25 species. In addition, some basic biological information on 64 fish species is also summarized. These results will be useful for fishery research, management and conservation in the Wujiang River.
      PubDate: 2016-02-06T01:05:31.449819-05:
      DOI: 10.1111/jai.13016
  • Confirmation of the occurrence of the toothpony Gazza minuta (Bloch, 1795)
           (Perciformes: Leiognathidae) in the Persian Gulf
    • PubDate: 2016-02-04T04:37:06.733358-05:
      DOI: 10.1111/jai.13011
  • Age, growth and reproduction of the sand smelt Atherina boyeri Risso, 1810
           in Mellah Lagoon (Eastern Algeria)
    • Authors: A. S. Boudinar; L. Chaoui, M. H. Kara
      Abstract: This aim of this paper was the study of the reproductive biology and growth of the sand smelt, Atherina boyeri, in Mellah Lagoon (Algeria). These data are important for the sustainable exploitation of the stocks of this species. Examined was a total of 1402 Atherina boyeri specimens captured monthly from March 2010 to March 2011, in a population with a 3‐year life cycle. Length–weight relationship was estimated as W = 0.0047 L3.077 (r2 = 0.935) for males and W = 0.0047 L3.176 (r2 = 0.935) for females. Using scales, the von Bertalanffy growth function fitted to back‐calculated size‐at‐age data was Lt = 9.49 [1 − e−0.316 (t + 0.928)] for males, and Lt = 11.67 [1 − e−0.179 (t + 1.514)] for females; using otoliths this was Lt = 9.68 [1 − e−0.3 (t + 1.02)] for males, and Lt = 11.93 [1 − e−0.171 (t + 1.55)] for females. The growth performance index (Φ) indicated that males (Φscales = 3.34, Φotoliths = 3.33) grew at the same rate as females (Φscales = 3.19, Φotoliths = 3.24), with a sex ratio of 1 : 1.6 in favor of females. The reproductive season extended from February to June. Individual length at first sexual maturity was 4.20 cm for 1‐year‐old males and 4.35 cm for 1‐year‐old females.
      PubDate: 2016-02-04T04:36:47.515413-05:
      DOI: 10.1111/jai.12992
  • Occurrence of Heniochus intermedius Steindachner, 1893 in Iskenderun Bay,
    • PubDate: 2016-02-04T04:35:38.386093-05:
      DOI: 10.1111/jai.13013
  • Length–weight relationships of three fish species from the Jinsha
           River, southwestern China
    • Authors: C. X. Xia; L. Chen, W. Xiong
      Abstract: Length–weight relationships were determined for the first time for three fish species (Xenocypris fangi Tchang, 1930; Microphysogobio kiatingensis Wu, 1930; and Opsariichthys bidens Günther, 1873) from the Jinsha River, southwestern China. Samples were collected using gillnets (30 × 15 m, mesh‐size 5 mm) and electroshock fishing techniques (CWB‐2000 P, 12 V, 250 Hz).
      PubDate: 2016-02-04T04:35:18.410155-05:
      DOI: 10.1111/jai.13014
  • Length–weight relationships for nine Paraschistura species from
           several river basins of Iran (Teleostei: Nemacheilidae)
    • Authors: G. Sayyadzadeh; H. R. Esmaeili
      Abstract: Length–weight parameters were estimated for nine nemacheilid fish of the genus Paraschistura from Iran. Significant length–weight relationships with high correlation coefficients were found for all studied species.
      PubDate: 2016-02-04T04:34:57.157946-05:
      DOI: 10.1111/jai.13017
  • Molecular phylogeny of three mudskippers (Gobiidae) from the Persian Gulf
           and Gulf of Oman (Makran)
    • Authors: M. Ghanbarifardi; H. R. Esmaeili, Z. Gholami, M. Aliabadian, B. Reichenbacher
      Abstract: Presented is a phylogenetic reconstruction of mudskippers using sequences of the mitochondrial COI and cytb genes. The phylogeny combines the sequences of 12 species from GenBank with those of three species (Boleophthalmus dussumieri, Periophthalmus waltoni and Scartelaos tenuis) collected from the coastal area of the Persian Gulf and Oman (Makran) Sea and sequenced for the first time. Based on the Maximum likelihood and Bayesian methods, Periophthalmus forms a separate clade that is the sister lineage to a group formed by all remaining genera. The subfamilies Oxudercinae and Amblyopinae established on the basis of morphological characters have been shown to be paraphyletic and their use as taxonomic units is no longer recommended. The evolution of Periophthalmus waltoni, Periophthalmus barbarous and Boleophthalmus dussumieri is discussed based on molecular phylogenetic reconstruction.
      PubDate: 2016-02-02T02:25:28.966596-05:
      DOI: 10.1111/jai.12999
  • Discard reduction of trammel nets in the Northeastern Mediterranean prawn
    • Abstract: In this study the effects on the discard reduction of guarding net rigged prawn trammel nets were examined with the focus on catch composition and income of fishermen for the Mersin Bay small‐scale prawn fishery. A total of 15 fishing trips were carried out between September and December 2011, using the commercial net and the alternative experimental net for comparison. The results revealed that catches of the main target species, green tiger prawn (Penaeus semisulcatus), in the alternative nets were only 1.5% smaller than those of the commercial nets, and with a statistically insignificant difference (P > 0.05). However, the catch of the main discarded species, the invasive swimming crab (Charybdes longicollis), mantis shrimp (Rissoides desmaresti), and the blue crab (Portunus pelagicus) were captured 85%, 66%, 75% less often in the alternative nets, with statistically significant differences (P 
      PubDate: 2016-02-02T02:25:08.546008-05:
      DOI: 10.1111/jai.13015
  • Length–weight relationships of four indigenous freshwater fish
           species from Khalsi wetland in lower Ganga basin, West Bengal, India
    • Authors: K. M. Sandhya; M. A. Hassan, S. Kumari, P. Mishal, L. Lianthuamluaia, V. Kumar, M. Aftabuddin, D. K. Meena
      Abstract: The length‐weight relationships of four indigenous freshwater fishes Parambassis lala (Hamilton, 1822), Trichogaster lalius (Hamilton, 1822), Puntius terio (Hamilton, 1822), and Pethia phutunio (Hamilton, 1822) were determined. Fish samples were collected during December 2013 to May 2015 from Khalsi, a floodplain wetland along the tributary of the River Ganges in West Bengal state, India using different types of gear (castnets, gillnets and drag nets, stretched mesh sizes of 15–20 mm, 20–30 mm and 5–10 mm, respectively). This is the first report on the length–weight relationship parameters of these four species.
      PubDate: 2016-02-02T02:24:28.853964-05:
      DOI: 10.1111/jai.13005
  • Length–weight relationships of seven fish species from the upper
           Pelus River, Kuala Kangsar district, Perak, Malaysia
    • Authors: M. E. M. Ikhwanuddin; M. N. A. Amal, S. Shohaimi, A. Azizul, S. Johari, T. Abdullah, N. R. Jamil
      Abstract: Length–weight relationships (LWR) are reported for seven fish species from the upper Pelus River, Kuala Kangsar district, Perak, Malaysia. This river is located in a remote area and supplies fishes for the aboriginal peoples. Fish samplings were conducted in three different seasons, the dry (June 2014), wet (August 2014), and moderately wet (April 2015). All samples were collected using fish electro–shocker and scoop nets with a 1 cm mesh size. A total of seven LWRs were unknown to FishBase, and four new maximum fish lengths were identified in the study.
      PubDate: 2016-01-25T05:28:29.860658-05:
      DOI: 10.1111/jai.13009
  • Issue Information
    • First page: 249
      PubDate: 2016-03-09T08:41:28.158375-05:
      DOI: 10.1111/jai.12884
  • Strontium metabolism in the juvenile Lake Sturgeon, Acipenser fulvescens
           (Rafinesque, 1817), and further evaluation of the isotope as a marking
           tool for stock discrimination
    • Authors: B. Carriere; D. Gillis, N. Halden, G. Anderson
      First page: 258
      Abstract: Stock enhancement efforts have been used to aid in increasing dwindling population levels of Lake Sturgeon, Acipenser fulvescens, that are currently of conservation concern. Consistent monitoring of stock enhanced fisheries requires a reliable and reproducible method to mark stocked fish such that the success of such programs can be determined. Recently, stable isotopic marking of hard structures in fish has been developed in a variety of species, however, optimal marking conditions are less well understood. In this study the metabolism of strontium in juvenile Lake Sturgeon and determined parameters for optimisation of marking success in the fin ray were examined. Using radioactive strontium (85Sr), whole body influx and efflux rates of strontium were 3.02 ± 0.364 pmol h−1 g−1 (mean ± standard error) and 0.04 ± 0.007 pmol h−1 g−1, respectively. Furthermore, short‐term accumulation of 85Sr in a variety of tissues was assessed and found to be greatest in the fin ray. Biological half‐life of the stable isotope 86Sr was shortest in muscle and longest in the fin ray tissue. Immersion and length of immersion timing had positive relationships with marking success. Additionally, the signature remained in 75% of fish analysed 550 days post‐mark. Data suggest that marking success was determined by duration of immersion in the mark and concentration of the mark introduced to the water.
      PubDate: 2016-01-27T08:31:28.448294-05:
      DOI: 10.1111/jai.13019
  • Effects of acute temperature change on the metabolism and swimming ability
           of juvenile sterlet sturgeon (Acipenser ruthenus, Linnaeus 1758)
    • Authors: P. Mandal; L. Cai, Z. Tu, D. Johnson, Y. Huang
      First page: 267
      Abstract: The objective of this study was to provide information on changes in the metabolism and swimming ability of juvenile sterlet sturgeon, Acipenser ruthenus, caused by acutely low or high temperatures. Changes in critical swimming speed (Ucrit), oxygen consumption rate (MO2), tail beat frequency (TBF) and tail beat amplitude (TBA) were observed with a Steffensen‐type swimming respirometer, an oxygen electrode and a camera at different swimming speeds at three temperatures: 5°C, 15°C, and 25°C. Fish tested at 5°C and 25°C were maintained at 15°C (near optimal) for one week to simulate conditions below a dam. The Ucrit value decreased significantly during acute temperature changes at 5°C and 25°C; Ucrit was highest near the optimal temperature. Oxygen consumption rate (MO2) increased with the swimming speed at 15°C; however, at 25°C and 5°C, the MO2 decreased with the swimming speed. Both TBA and TBF decreased at 5°C and 25°C compared to values at 15°C. The slopes of the regression lines (TBF/U) at 5°C and 25°C seemed lower compared to 15°C.
      PubDate: 2016-03-06T23:20:33.135704-05:
      DOI: 10.1111/jai.13033
  • Molecular characterization and expression of Kiss2/Kiss2r during embryonic
           and larval development in (Megalobrama amblycephala Yih, 1955)
    • First page: 288
      Abstract: In order to test whether Kiss/Kissr systems have potential roles in regulating the embryonic and larval development in teleosts, in this study the Kiss2/Kiss2r full‐length cDNA was cloned from blunt snout bream (Megalobrama amblycephala Yih, 1955) by rapid amplification of cDNA ends and their expression patterns were detected in different tissues of adult and developmental stages for the embryonic and larval periods via quantitative real‐time PCR. Both Kiss and Kissr genes full‐length cDNA sequences of M. amblycephala were obtained and phylogenetic analysis results indicated that these genes belong to the Kiss2/Kiss2r clade. Bioinformatics analyses revealed that there was a conserved decapeptide in M. amblycephala Kiss2 gene putative amino acids, but only two transmembrane domains were predicated in Kiss2r. Tissue distribution analyses showed that both genes were widely expressed in the tissues tested, with high levels in the muscle, gonad and pituitary. At different developmental stages, the mRNA expression of Kiss2/Kiss2r was highest in the blastocyst/15 hpf stage and lowest in the 30 hpf /blastocyst stages for the embryonic period, highest in 7 dph/15 dph and lowest in 30 dph/30 dph for larval period, respectively. These results suggest that the Kiss2/Kiss2r system has varied potential for influencing embryonic and larval development in fish species.
      PubDate: 2016-01-25T05:31:03.891286-05:
      DOI: 10.1111/jai.13008
  • Growth performance and body composition in response to dietary protein and
           lipid levels in Nile tilapia (Oreochromis niloticus Linnaeus, 1758)
           subjected to normal and temporally restricted feeding regimes
    • First page: 332
      Abstract: A factorial experiment was designed to examine the effect on compensatory growth (CG) of Nile tilapia Oreochromis niloticus fed diets containing different protein and lipid levels under normal and temporally restricted feeding regimes. Four diets were formulated to contain either 30% or 36% crude protein, and 5% or 11% crude lipid. Triplicate replicates of each treatment were assigned to 24 150‐L tanks (20 fish/tank density). Fish (mean initial weight ± SD = 8.79 ± 0.34 g) were then fed either the normal feeding regime (thrice daily to apparent satiation) or the restricted regime (1 day feed deprivation followed by 3 days of feeding to apparent satiation) over a 44‐days study period. Fish receiving a diet under the restricted regime achieved weight gains (WG) comparable to fish consuming the diet containing 30% protein and 5% lipids under the normal feeding regime. Fish maintained on the restricted feeding regime exhibited reduced feed intake (FI), WG, feed efficiency ratio (FE), protein efficiency rate (PER) and hepatosomatic index versus fish on the normal feeding regime, except WG in fish fed the diet with 30% protein and 5% lipids. However, the resultant FI (85%~94%) was higher than the excepted 75% intake when fish were subjected to the restricted regime. Feeding 11% lipid diets led to improved FI, WG, FE, and PER compared to feeding the 5% lipid diets. Increased FI, WG, and FE, but reduced PER were observed in fish fed with 36% protein versus fish fed 30% protein. Fish receiving the 36% protein diets had lower whole‐body moisture and ash contents, but elevated whole‐body protein and lipid contents compared to those receiving the 30% protein diets. Whole‐body moisture contents were lower, but whole‐body protein, lipid and ash contents were higher in fish fed 11% lipid diets than in fish fed 5% lipid diets. There was an increase in whole‐body moisture content, but a decrease in protein and lipid content in response to the restricted feeding regime. Ash content was not affected by the feeding regime. The present study shows that Nile tilapia fed diets subjected to a restricted feeding regime exhibited growth comparable to those fed the diet at 30% protein and 5% lipid levels under a normal feeding regime. This positive effect was more pronounced in diets at a high protein level or in a combination of high protein and lipid levels.
      PubDate: 2016-01-29T00:18:01.282277-05:
      DOI: 10.1111/jai.13004
  • Effects of tank colour on feeding, growth and stress responses of young
           taimen Hucho taimen (Pallas, 1773)
    • First page: 339
      PubDate: 2016-01-25T05:30:38.567948-05:
      DOI: 10.1111/jai.12982
  • Relationships between fish size and otolith measurements for Chlorurus
           sordidus (Forsskål, 1775) and Hipposcarus harid (Forsskål, 1775)
           from the Red Sea coast of Egypt
    • First page: 356
      PubDate: 2016-01-21T01:16:43.07896-05:0
      DOI: 10.1111/jai.12995
  • On the occurrence of the bluefin driftfish Psenes pellucidus Lütken
           1880 (Perciformes, Nomeidae) in Tunisian waters (Mediterranean Sea)
    • Authors: R. Ghanem; M. Rifi, J. Ben Souissi, E. Azzurro
      First page: 359
      PubDate: 2016-01-27T08:32:07.136855-05:
      DOI: 10.1111/jai.12996
  • Rapid visual assessment of spawning activity and associated habitat
           utilisation of sea lamprey (Petromyzon marinus Linnaeus, 1758) in a chalk
           stream: implications for conservation monitoring
    • Authors: A. C. Pinder; E. Hopkins, L. J. Scott, J. R. Britton
      First page: 364
      PubDate: 2016-01-29T00:18:27.995811-05:
      DOI: 10.1111/jai.13010
  • Effects of salinity on growth, body composition, muscle fatty acid
           composition, and antioxidant status of juvenile Nile tilapia Oreochromis
           niloticus (Linnaeus, 1758)
    • Authors: L. Gan; Z. X. Xu, J. J. Ma, C. Xu, X. D. Wang, K. Chen, L. Q. Chen, E. C. Li
      First page: 372
      PubDate: 2016-01-21T01:30:36.604072-05:
      DOI: 10.1111/jai.12997
  • Length–weight and length–length relationships of 139
           Indo‐Pacific fish species (Teleostei) from the Davao Gulf,
    • First page: 377
      Abstract: Length–weight relationships (LWRs) of 139 coral reef and pelagic fish species (representing 34 fish families) were calculated based on 3806 individuals measured at local fish markets near the Davao Gulf in the southern Philippines during weekly visits between March 2009 and July 2011, as well as in June 2012. Fishes were caught with a variety of fishing methods, corroborated by abrasions and injuries. Forty‐seven of 139 LWRs were firstly reported and new to science. The mean slope b of the LWRs was 3.035, indicating that the majority of studied species followed isometric growth. Standard length – total length relationships were calculated for all measured fish species. Additionally, standard length – fork length relationships are presented for 108 species. Moreover, fifteen new records of maximum fish length and weight are reported.
      PubDate: 2016-01-25T05:30:20.066672-05:
      DOI: 10.1111/jai.12993
  • Length–weight relationships of three fish species from the upper
           reaches of the Beijiang River, Shaoguan, China
    • First page: 395
      Abstract: Fish assemblages were randomly collected in March 2015 from the upper reaches of the Beijiang River, Shaoguan, China. Fish were sampled on 10 occasions using gill nets (mesh size: 3 cm) and the length–weight relationships (LWRs; W = a × Lb) of three native species recorded: Xenocypris davidi, Xenocypris macrolepis and Culter recurviceps. The exponent b varied from 2.803 to 3.096 and r2 values ranged 0.985–0.992. This is the first report on the LWRs of fish from this area, with valuable results for the research, management and conservation of fishery resources in the upper reaches of the Beijiang River.
      PubDate: 2016-01-21T01:13:31.540062-05:
      DOI: 10.1111/jai.12989
  • Application of data‐limited assessment methods on black anglerfish
           (Lophius budegassa, Spinola, 1807) stocks in the Mediterranean Sea
    • Authors: P. Vasilakopoulos; C. D. Maravelias
      First page: 277
      Abstract: A survey‐based assessment of an eastern Mediterranean data‐limited black anglerfish (Lophius budegassa; Spinola, 1807) stock was carried out to elucidate its population and exploitation trends. A catch‐based method was also applied to estimate its maximum sustainable yield (MSY). The effect on the long‐term spawning stock biomass and yield of a wide range of exploitation regimes (combinations of F and selectivity) was investigated using an age‐structured population model parameterised for Mediterranean anglerfish stocks. The analysis indicated an increasing trend of anglerfish fishing mortality (F) in the eastern Mediterranean from the mid‐1990s onwards, and that recent catches were 41% higher than the median MSY estimate. Catching Mediterranean anglerfish more than 3 years after they mature at an F = 0.4–1 year−1 would ensure high yields at sustainable levels of stock depletion. Examination of the empirical exploitation regimes in five anglerfish stocks across the Mediterranean Sea illustrates their unfulfilled potential for higher sustainable yields, mainly due to overexploitation of juveniles.
      PubDate: 2015-12-31T01:23:14.351623-05:
      DOI: 10.1111/jai.12968
  • Investigations on the reproductive biology and diet of yellowfin tuna,
           Thunnus albacares, (Bonnaterre, 1788) in the Oman Sea
    • Authors: S. A. Hosseini; F. Kaymaram
      First page: 310
      Abstract: In this paper, the examination of some reproductive features and stomach contents of yellowfin tuna, Thunnus albacares, were determined by samples taken from Iranian drifting gillnets in the Oman Sea in the years 2007 to 2009. The male to female ratio in overall size was 1 : 0.93, which was not significantly different from the expected value 1 : 1. Fish lengths ranged from 37 to 152 cm, averaging 78.5 cm for females and 79.2 cm for males. At a larger size (>117 cm) males were proportionally predominant. Length at first maturity, Lm 50%, was observed when females reached 77.2 cm. Data from maturity stages indicated a single spawning period, peaking in May‐June, corresponding with a drop in the Gonadosomatic Index (GSI). Fishes were the main prey group, comprising 47.6% of total numbers among the three food item categories in the tuna stomachs. Among the 12 families identified in the stomachs, the Portunidae swimming crab crustacean was the most important prey organism (18.7% of total numbers). A wide range of prey species found in yellowfin tuna stomachs reflects an opportunistic feeding behaviour restricted by local prey availability. Considering the scarcity of available data, the results of the present paper will provide a reference for better knowledge of the biological features of T. albacares in the Oman Sea.
      PubDate: 2015-12-21T04:56:47.188283-05:
      DOI: 10.1111/jai.12907
  • New data on the longevity of coastal cod Gadus morhua Linnaeus, 1758 in
           the White Sea
    • Authors: P. N. Yershov; C. Marschal, A. V. Ereskovsky, A. E. Vishnyakov
      First page: 350
      Abstract: The cod, Gadus morhua, is a common and abundant demersal fish in the White Sea coastal zone. The published data on the maximum age of White Sea cod still requires additional documentation, because some authors have noted difficulties in age determination by otoliths of large specimens. To obtain accurate data on the longevity of this species in the White Sea, an image analysis of thin‐sections of otoliths was made for age estimation. Research surveys in the Chupa Inlet and adjacent waters of the Kandalaksha Bay were conducted in June to August from 2007 to 2013. The five largest specimens of a total of 3564 captured fish were selected for age determination. Age of these largest individuals ranged between 7 and 12 years, total length and weight varied from 60.2 to 77.0 cm and from 2.4 to 6.1 kg, respectively. According to the data, maximal White Sea cod longevity is greater than in previously published data, and demonstrates similar longevity to conspecifics from the Baltic and North seas, whereas its life cycle is much shorter than cod from the Northeast Arctic, Iceland, Greenland, Newfoundland and Labrador stocks.
      PubDate: 2015-11-25T05:41:08.050668-05:
      DOI: 10.1111/jai.12961
  • Testing staining techniques to determine age and growth of Dasyatis
           pastinaca (Linnaeus, 1758) captured in Iskenderun Bay, northeastern
    • Abstract: This study tested the suitability of several staining methods to determine the age of common stingray (Dasyatis pastinaca) from Iskenderun Bay, Turkey. A total of 384 specimens (16.6 cm–69.3 cm disc width) were obtained by trawling between September 2010 and December 2011. Sex ratio of the samples was 53% males and 47% females. Appropriate age determination was firstly demonstrated using Safranin‐O staining. Age readings were made by two independent readers and the index of average percent error (IAPE) determined as 6.3% for Safranin‐O, 6.8% for Crystal Violet, 7.9% for Alcian Blue, and 9.3% for Silver Nitrate. Safranin‐O and Crystal Violet staining methods provided the best results. Verification of temporal growth ring formation was by marginal increment analysis. Disc width–weight relationships were determined by W = 0.0272*DW3.06 for females and W = 0.0247*DW3.08 for males. Estimates of the von Bertalanffy growth parameters indicated a larger asymptotic disc width (DW∞ = 127.06 cm) for females than for males (DW∞ = 114.54 cm); growth parameters were k = 0.058 year−1, to = −1.508 and k = 0.041 year−1, to = −3.632 for females and males, respectively.
  • Estimation of genetic parameters for disease‐resistance traits in
           Cynoglossus semilaevis (Günther, 1873)
    • Abstract: The objective of this study was to estimate genetic parameters for three traits based on 28 Cynoglossus semilaevis families approximately 6 months of age (at least 5 cm total length), including trait_1 (survival of 26 families, 3434 individuals in total subjected to challenge tests with Edwardsiella tarda), trait_2 (survival of 20 families, 2016 individuals in total subjected to challenge tests with Vibrio anguillarum) and trait_3 (survival of 27 families, 9340 individuals tagged at circa 180 days of age and reared in indoor ponds for circa another 5 months). The result showed that there were large differences in the survival of the families after challenge (11.11–65.31% for E. tarda and 9.18–70.54% for V. anguillarum). Additionally, the survival of families reared in indoor ponds was also different, varying from 21.00% to 73.67%. Heritabilities of the three traits varied from 0.14 to 0.26, as estimated by the linear model (LM) and the threshold model (TM). The trait_1 heritabilities (0.26 and 0.19 estimated by LM and TM) were higher than those of the others (0.20 and 0.23 estimated by LM, 0.14 and 0.19 estimated by TM). The estimates of heritabilities using LM were consistently higher than those of TM in this study. There were significant medium genetic correlations of 0.44 and 0.42 between trait_1 and trait_2 obtained from LM and TM (P 
  • Population structure, length–weight relationship and growth of white
           seabream, Diplodus sargus sargus (Linneaus, 1758), in Beymelek Lagoon,
    • Abstract: The main objective of this study was to investigate the population structure, length–weight relationship (LWR) and growth of white seabream, Diplodus sargus sargus (L., 1758) in Beymelek Lagoon, Turkey. Samples were collected by gill‐ and trammel nets of various mesh sizes, February 2006 to January 2007. A total of 355 specimens (51 males; 34 females; 270 unidentified) were collected. Male to female ratio was 1.50 : 1. Ages ranged from 0 to 3 years; about 70% were juveniles. Most fish were between 13 and 16 cm in total length and below 90 g in weight. The LWR of all individuals was W = 0.0140 × L3.1028. The von Bertalanffy growth equation was calculated as Lt = 39.9 × [1 – exp(−0.268 × (t + 1.753)] and the growth performance index (Φ′) was 6.056.
  • Length–weight relationships for 10 elasmobranch species from the
           Oman Sea
    • Abstract: Length–weight relationships (LWRs) were estimated for ten elasmobranch species from Iranian waters of the Oman Sea. All specimens were collected by bottom trawl (mesh size 80 mm in the cod end) between May and December 2014. A total of 509 individuals (107 Torpedo sinuspersici, 68 Rhinobatos punctifer, 63 Chaenogaleus macrostoma, 72 Himantura walga, 80 Himantura gerrardi, 58 Gymnura poecilura, 4 Himantura uarnak, 4 Rhinoptera javanica, 14 Aetobatus flagellum, and 39 Pastinachus sephen) were sampled and studied. Results showed that most species had positive allometric or isometric growth but that more studies were needed for final decisions on growth patterns. Both the disk length‐body weight (DL/BW) and disk width‐body weight (DW/BW) relationships also showed a good fit, reinforcing the credibility of the data in relation to the LWRs. This study is the first report of LWRs for these elasmobranchs in the northern Oman Sea.
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