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  Subjects -> BIOLOGY (Total: 2708 journals)
    - BIOCHEMISTRY (207 journals)
    - BIOENGINEERING (82 journals)
    - BIOLOGY (1344 journals)
    - BIOPHYSICS (46 journals)
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BIOLOGY (1344 journals)            First | 1 2 3 4 5 6 7 8 | Last

Drug Resistance Updates     Hybrid Journal   (Followers: 3)
e-Neuroforum     Hybrid Journal  
Ecohydrology & Hydrobiology     Full-text available via subscription   (Followers: 5)
Ecological Engineering     Hybrid Journal   (Followers: 2)
Ecology and Society     Open Access   (Followers: 26)
Ecology Letters     Hybrid Journal   (Followers: 339)
Economics & Human Biology     Hybrid Journal  
Ecoprint : An International Journal of Ecology     Open Access  
Ecoscience     Full-text available via subscription   (Followers: 2)
Educational Technology Research and Development     Partially Free   (Followers: 206)
Egyptian Journal of Biology     Open Access  
Egyptian Journal of Histology     Partially Free   (Followers: 1)
Egyptian Journal of Natural History     Open Access   (Followers: 1)
EJNMMI Research     Open Access   (Followers: 1)
Electromagnetic Biology and Medicine     Hybrid Journal   (Followers: 1)
eLife     Open Access   (Followers: 17)
el–Hayah     Open Access   (Followers: 1)
Embo Molecular Medicine     Open Access   (Followers: 3)
EMBO reports     Full-text available via subscription   (Followers: 17)
Emotion Review     Hybrid Journal   (Followers: 11)
Endocrine Connections     Open Access   (Followers: 4)
Engineering & Technology     Hybrid Journal   (Followers: 17)
Engineering Economist, The     Hybrid Journal   (Followers: 2)
Engineering in Life Sciences     Hybrid Journal   (Followers: 4)
Engineering Optimization     Hybrid Journal   (Followers: 6)
Ensaios e Ciência: Ciências Biológicas, Agrárias e da Saúde     Open Access  
Environmental Biology of Fishes     Hybrid Journal   (Followers: 4)
Environmental Biosafety Research     Hybrid Journal   (Followers: 1)
Environmental Microbiology     Hybrid Journal   (Followers: 8)
Environmental Science & Technology     Full-text available via subscription   (Followers: 307)
Enzyme and Microbial Technology     Hybrid Journal   (Followers: 5)
Enzyme Research     Open Access   (Followers: 4)
Epidemiology & Infection     Hybrid Journal   (Followers: 12)
Epigenetics in Cancer     Open Access  
EPMA Journal     Open Access  
ESHRE Monographs     Hybrid Journal  
Ethiopian Journal of Biological Sciences     Open Access   (Followers: 2)
Ethnobiology and Conservation     Open Access   (Followers: 2)
Ethnobotany Research & Applications : a journal of plants, people and applied research     Open Access   (Followers: 5)
Ethology     Hybrid Journal   (Followers: 5)
Ethology Ecology & Evolution     Hybrid Journal   (Followers: 10)
Eukaryotic Cell     Full-text available via subscription   (Followers: 6)
EuPA Open Proteomics     Open Access   (Followers: 1)
EURASIP Journal on Bioinformatics and Systems Biology     Open Access   (Followers: 6)
European Journal of Cell Biology     Hybrid Journal   (Followers: 3)
European Journal of Neuroscience     Hybrid Journal   (Followers: 24)
European Journal of Phycology     Hybrid Journal   (Followers: 5)
European Journal of Protistology     Hybrid Journal   (Followers: 2)
European Journal of Soil Biology     Hybrid Journal   (Followers: 1)
European Online Journal of Natural and Social Sciences     Open Access   (Followers: 12)
European Scientific Journal     Open Access   (Followers: 2)
Evidência - Ciência e Biotecnologia - Interdisciplinar     Open Access  
EvoDevo     Open Access   (Followers: 1)
Evolução e Conservação da Biodiversidade     Open Access  
Evolution     Partially Free   (Followers: 62)
Evolution and Human Behavior     Hybrid Journal   (Followers: 10)
Evolutionary Applications     Open Access   (Followers: 3)
Evolutionary Bioinformatics     Open Access   (Followers: 15)
Evolutionary Biology     Hybrid Journal   (Followers: 17)
Evolutionary Computation     Hybrid Journal   (Followers: 9)
Experimental & Molecular Medicine     Open Access   (Followers: 2)
Experimental and Applied Acarology     Hybrid Journal   (Followers: 1)
Experimental Cell Research     Hybrid Journal   (Followers: 3)
Expert Opinion on Biological Therapy     Hybrid Journal   (Followers: 10)
Expert Opinion on Environmental Biology     Partially Free  
Expert Review of Proteomics     Full-text available via subscription   (Followers: 2)
Extreme Life, Biospeology & Astrobiology - International Journal of the Bioflux Society     Full-text available via subscription   (Followers: 4)
Extremophiles     Hybrid Journal   (Followers: 1)
F1000Research     Open Access   (Followers: 3)
Facta Universitatis, Series : Medicine and Biology     Open Access  
Familial Cancer     Hybrid Journal   (Followers: 1)
Fatigue & Fracture of Engineering Materials and Structures     Hybrid Journal   (Followers: 13)
Fauna Norvegica     Open Access  
Febs Journal     Hybrid Journal   (Followers: 8)
Feddes Repertorium     Hybrid Journal  
Fems Yeast Research     Hybrid Journal   (Followers: 10)
Field Mycology     Full-text available via subscription   (Followers: 1)
Fish & Shellfish Immunology     Hybrid Journal   (Followers: 8)
Fitoterapia     Hybrid Journal   (Followers: 4)
Fly     Full-text available via subscription  
Folia Biologica     Full-text available via subscription  
Folia Biologica et Oecologica     Open Access   (Followers: 1)
Folia Histochemica et Cytobiologica     Open Access  
Folia Malacologica     Open Access  
Folia Microbiologica     Hybrid Journal   (Followers: 1)
Folia Primatologica     Full-text available via subscription   (Followers: 1)
Food and Bioproducts Processing     Hybrid Journal   (Followers: 2)
Forest Pathology     Hybrid Journal   (Followers: 1)
Forschung     Hybrid Journal  
Foundations of Physics     Hybrid Journal   (Followers: 1)
Free Radical Biology and Medicine     Hybrid Journal   (Followers: 5)
Free Radical Research     Hybrid Journal   (Followers: 2)
Freshwater Reviews     Full-text available via subscription   (Followers: 3)
Freshwater Science     Full-text available via subscription   (Followers: 6)
Frontiers in Ecology and Evolution     Open Access   (Followers: 4)
Frontiers in Evolutionary Neuroscience     Open Access   (Followers: 1)
Frontiers in Life Science     Hybrid Journal  
Frontiers in Marine Science     Open Access   (Followers: 1)
Frontiers in Neurogenesis     Open Access  
Frontiers in Neuroprosthetics     Open Access   (Followers: 1)

  First | 1 2 3 4 5 6 7 8 | Last

Journal Cover Anaerobe     [SJR: 0.828]   [H-I: 39]
   [5 followers]  Follow    
   Hybrid Journal Hybrid journal (It can contain Open Access articles)
   ISSN (Print) 1075-9964 - ISSN (Online) 1095-8274
   Published by Elsevier Homepage  [2585 journals]
  • High-throughput 16S rRNA gene sequencing reveals alterations of intestinal
           
    • Abstract: Publication date: August 2013
      Source:Anaerobe, Volume 22
      Author(s): Marc Frémont , Danny Coomans , Sebastien Massart , Kenny De Meirleir
      Human intestinal microbiota plays an important role in the maintenance of host health by providing energy, nutrients, and immunological protection. Intestinal dysfunction is a frequent complaint in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) patients, and previous reports suggest that dysbiosis, i.e. the overgrowth of abnormal populations of bacteria in the gut, is linked to the pathogenesis of the disease. We used high-throughput 16S rRNA gene sequencing to investigate the presence of specific alterations in the gut microbiota of ME/CFS patients from Belgium and Norway. 43 ME/CFS patients and 36 healthy controls were included in the study. Bacterial DNA was extracted from stool samples, PCR amplification was performed on 16S rRNA gene regions, and PCR amplicons were sequenced using Roche FLX 454 sequencer. The composition of the gut microbiota was found to differ between Belgian controls and Norwegian controls: Norwegians showed higher percentages of specific Firmicutes populations (Roseburia, Holdemania) and lower proportions of most Bacteroidetes genera. A highly significant separation could be achieved between Norwegian controls and Norwegian patients: patients presented increased proportions of Lactonifactor and Alistipes, as well as a decrease in several Firmicutes populations. In Belgian subjects the patient/control separation was less pronounced, however some abnormalities observed in Norwegian patients were also found in Belgian patients. These results show that intestinal microbiota is altered in ME/CFS. High-throughput sequencing is a useful tool to diagnose dysbiosis in patients and could help designing treatments based on gut microbiota modulation (antibiotics, pre and probiotics supplementation).


      PubDate: 2014-12-18T14:11:13Z
       
  • Identification of pili on the surface of Finegoldia magna – A
           Gram-positive anaerobic cocci
    • Abstract: Publication date: June 2014
      Source:Anaerobe, Volume 27
      Author(s): Elizabeth C. Murphy , Robert Janulczyk , Christofer Karlsson , Matthias Mörgelin , Inga-Maria Frick
      Pili have only been discovered in the major Gram-positive pathogens in the past decade and they have been found to play an important role in colonisation and virulence. Pili have been shown to have many important functions including attachment to host tissues, mediating bacterial aggregation, biofilm formation and binding to proteins in the extracellular matrix. In this study, sortase-dependent pili have been found to be expressed on the surface of Finegoldia magna ALB8. F. magna is a Gram-positive anaerobic coccus that, primarily, is a commensal of the skin and mucous membranes, but has also been isolated from various clinical infection sites and is associated with soft-tissue abscesses, wound infections and bone and prosthetic joint infections. In this study, F. magna ALB8 was found to harbour three sortases at the pilus locus, two of which bear high similarity to class C sortases in Streptococcus pneumoniae. Two putative sortase-dependent pili proteins were found in the locus, with one being identified as the major pilus subunit, Fmp1 (F. magna pilus subunit 1), due to its high similarity to other major pilus proteins in prominent Gram-positive pathogens. The presence of sortase-dependent pili was confirmed experimentally through recombinant production of Fmp1 and production of antiserum. The Fmp1 antiserum was used in Western blot to show the presence of a high molecular weight protein ladder, characteristic of the presence of pili, in trypsin released cell wall surface proteins from F. magna. The presence of sortase-dependent pili was visually confirmed by transmission electron microscopy, which showed the binding of gold labelled anti-Fmp1 to individual pilus proteins along the pilus. Furthermore, pili could also be found to bind and interact with keratinocytes in the epidermal layer of human skin, suggesting an adhesive role for pili on F. magna. Our work represents the first description of pilus structures in F. magna. This discovery further elucidates F. magna physiology and allows for additional analysis of host–bacterial interactions in future studies.


      PubDate: 2014-12-18T14:11:13Z
       
  • Clostridium perfringens and Clostridium difficile in cooked beef sold in
           Côte d'Ivoire and their antimicrobial susceptibility
    • Abstract: Publication date: August 2014
      Source:Anaerobe, Volume 28
      Author(s): Kra Athanase Kouassi , Adjéhi Thomas Dadie , Kouadio Florent N'Guessan , Koffi Marcellin Dje , Yao Guillaume Loukou
      The aim of this study was to evaluate the prevalence of Clostridium difficile and Clostridium perfringens in cooked beef sold in the streets in Côte d'Ivoire and their antimicrobial susceptibility. A total of 395 kidney and flesh samples of cooked beef were collected from vendors at Abidjan and subjected to C. difficile and C. perfringens isolation and identification by using biochemical tests, API 20A system and PCR detection. Subsequently, the antimicrobial susceptibility test was performed for confirmed isolates. Our results showed the prevalence of 12.4% for C. difficile (11.04% in kidney and 13.45% in flesh) and 5.06% for C. perfringens (2.32% in kidney and 7.17% in flesh). Metronidazole and vancomycin remained the most potent antimicrobial agents against C. difficile while metronidazole and penicillin G were the most potent agents against C. perfringens. The resistance rates to tetracycline, doxycycline, chloramphenicol and erythromycin against C. difficile and C. perfringens isolates ranged from 2.05% to 8.16% and from 20% to 50%, respectively. Among all antimicrobial agents tested against C. difficile, percentages of resistance to quinolones ciprofloxacin, norfloxacin and nalidixic acid as well as to gentamicin and cefotaxime were the highest. Eight resistant phenotypes were defined for C. difficile isolates and eleven resistant phenotypes for C. perfringens isolates. Clindamycin/gentamicin/cefotaxime/ciprofloxacin/norfloxacin/nalidixic acid resistance was the most common phenotype for C. difficile (55.10% of isolates) while norfloxacin/nalidixic acid resistance was the most common phenotype for C. perfringens (20% of isolates).


      PubDate: 2014-12-18T14:11:13Z
       
  • Antifungal activity and identification of Lactobacilli, isolated from
           traditional dairy product “katak”
    • Abstract: Publication date: August 2014
      Source:Anaerobe, Volume 28
      Author(s): Rositsa Tropcheva , Dilyana Nikolova , Yana Evstatieva , Svetla Danova
      Filamentous moulds are the main spoilage microorganisms, responsible for significant economic losses and several healthy risks in human food chain. The lactic acid bacteria (LAB), especially lactobacilli could be a natural antagonist of these dangerous organisms. In Bulgaria, a very limited data exists on the antifungal activity of LAB microbiota of fermented dairy products. In the present study, four active strains were isolated from traditional fermented curd/yogurt-like product “katak”, produced in Bulgaria from centuries. The new isolates KR3, KR4, KR51 and KR53 were identified by API 50 CH biochemical test and different molecular methods (species-specific PCR, RAPD-PCR and 16S rDNA sequence analysis) as Lactobacillus brevis. According to our knowledge, this is the first data on the molecular characterization of the Lactobacillus microbiota of “katak”. A broad spectrum of antifungal activity of the four L. brevis KR strains against test-cultures representatives of carcinogenic, toxigenic, deteriorative and allergenic fungi from the genera Aspergillus, Fusarium, Penicillium and Trichoderma was estimated. Strains L. brevis KR3, KR4 and KR51 completely suppress the growth of Penicillium claviforme, Aspergillus awamori and Aspergillus niger. With regard to Aspergillus flavus and Trichoderma viride, a lower and strain-specific inhibitory activity was observed. The antifungal activity of our new L. brevis isolates seems to be a promising advantage of these four strains, suggesting their potential applications in different food technologies as bio-preservative agents against moulds.


      PubDate: 2014-12-18T14:11:13Z
       
  • Comparison of culture based methods for the isolation of Clostridium
           difficile from stool samples in a research setting
    • Abstract: Publication date: August 2014
      Source:Anaerobe, Volume 28
      Author(s): Michelle Lister , Emma Stevenson , Daniela Heeg , Nigel P. Minton , Sarah A. Kuehne
      Effective isolation of Clostridium difficile from stool samples is important in the research setting, especially where low numbers of spores/vegetative cells may be present within a sample. In this study, three protocols for stool culture were investigated to find a sensitive, cost effective and timely method of C. difficile isolation. For the initial enrichment step, the effectiveness of two different rich media, cycloserine-cefoxitin fructose broth (CCFB) and cycloserine-cefoxitin mannitol broth with taurocholate and lysozyme (CCMB-TAL) were compared. For the comparison of four different, selective solid media; Cycloserine-cefoxitin fructose agar (CCFA), Cycloserine-cefoxitin egg yolk agar (CCEY), ChromID C. difficile and tryptone soy agar (TSA) with 5% sheep's blood with and without preceding broth enrichment were used. As a means to enable differentiation between C. difficile and other fecal flora, the effectiveness of the inclusion of a pH indictor (1% Neutral Red), was also evaluated. The data derived indicated that CCFB is more sensitive than CCMB-TAL, however, the latter had an improved recovery rate. A broth enrichment step had a reduced sensitivity over direct plating. ChromID C. difficile showed the best recovery rate whereas CCEY egg yolk agar was the most sensitive of the four. The addition of 1% Neutral Red did not show sufficient colour change when added to CCEY egg yolk agar to be used as a differential medium. For a low cost, timely and sensitive method of isolating C. difficile from stool samples we recommend direct plating onto CCEY egg yolk agar after heat shock.


      PubDate: 2014-12-18T14:11:13Z
       
  • A combined metabolomic and phylogenetic study reveals putatively prebiotic
           effects of high molecular weight arabino-oligosaccharides when assessed by
           in vitro fermentation in bacterial communities derived from humans
    • Abstract: Publication date: August 2014
      Source:Anaerobe, Volume 28
      Author(s): Karolina Sulek , Louise Kristine Vigsnaes , Line Rieck Schmidt , Jesper Holck , Henrik Lauritz Frandsen , Jørn Smedsgaard , Thomas Hjort Skov , Anne S. Meyer , Tine Rask Licht
      Prebiotic oligosaccharides are defined by their selective stimulation of growth and/or activity of bacteria in the digestive system in ways claimed to be beneficial for health. However, apart from the short chain fatty acids, little is known about bacterial metabolites created by fermentation of prebiotics, and the significance of the size of the oligosaccharides remains largely unstudied. By in vitro fermentations in human fecal microbial communities (derived from six different individuals), we studied the effects of high-mass (HA, >1 kDa), low-mass (LA, <1 kDa) and mixed (BA) sugar beet arabino-oligosaccharides (AOS) as carbohydrate sources. Fructo-oligosaccharides (FOS) were included as reference. The changes in bacterial communities and the metabolites produced in response to incubation with the different carbohydrates were analyzed by quantitative PCR (qPCR) and Liquid Chromatography–Mass Spectrometry (LC–MS), respectively. All tested carbohydrate sources resulted in a significant increase of Bifidobacterium spp. between 1.79 fold (HA) and 1.64 fold (FOS) in the microbial populations after fermentation, and LC–MS analysis suggested that the bifidobacteria contributed to decomposition of the arabino-oligosaccharide structures, most pronounced in the HA fraction, resulting in release of the essential amino acid phenylalanine. Abundance of Lactobacillus spp. correlated with the presence of a compound, most likely a flavonoid, indicating that lactobacilli contribute to release of such health-promoting substances from plant structures. Additionally, the combination of qPCR and LC–MS revealed a number of other putative interactions between intestinal microbes and the oligosaccharides, which contributes to the understanding of the mechanisms behind prebiotic impact on human health.


      PubDate: 2014-12-18T14:11:13Z
       
 
 
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